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Full\u2010mouth periodontal parameters were measured, and saliva (<jats:italic>n<\/jats:italic>\u2009=\u200930) collected pre\u2010 (T0), and one (T1) and six (T6) months post\u2010treatment. The proteome was investigated by label\u2010free quantitative proteomics. Protein expression changes were modelled over time, with significant protein regulation considered at false discovery rate &lt;0.05.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Treatment significantly reduced bleeding scores, percentages of sites with pocket depth \u22655\u2009mm, plaque and gingival indexes. One thousand seven hundred and thirteen proteins were identified and 838 proteins (human\u2009=\u2009757, bacterial\u2009=\u200981) quantified (\u22652 peptides). At T1, 80 (T1 vs. T0: 60\u2191:20\u2193), and at T6, 118 human proteins (T6 vs. T0: 67\u2191:51\u2193) were regulated. The salivary proteome at T6 versus T1 remained stable. Highest protein activity post\u2010 versus pre\u2010treatment was observed for cellular movement and inflammatory response. The small proline\u2010rich protein 3 (T1 vs. T0: 5.4\u2010fold\u2191) and lymphocyte\u2010specific protein 1 (T6 vs. T0: 4.6\u2010fold\u2193) were the top regulated human proteins. Proteins from <jats:italic>Neisseria mucosa<\/jats:italic> and <jats:italic>Treponema socranskii<\/jats:italic> (T1 vs. T0: 8.0\u2010fold\u2193, 4.9\u2010fold\u2193) were down\u2010regulated.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Periodontal treatment reduced clinical disease parameters and these changes were reflected in the salivary proteome. This underscores the potential of utilizing saliva biomarkers as prognostic tools for monitoring treatment outcomes.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jcpe.13990","type":"journal-article","created":{"date-parts":[[2024,4,25]],"date-time":"2024-04-25T08:19:39Z","timestamp":1714033179000},"page":"56-67","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Probing the salivary proteome for prognostic biomarkers in response to non\u2010surgical periodontal therapy"],"prefix":"10.1111","volume":"52","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3717-6969","authenticated-orcid":false,"given":"Angelika","family":"Silbereisen","sequence":"first","affiliation":[{"name":"Division of Oral Health and Periodontology, Department of Dental Medicine Karolinska Institutet  Stockholm 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