{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T23:07:15Z","timestamp":1791846435433,"version":"4.3.4"},"reference-count":33,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2024,5,12]],"date-time":"2024-05-12T00:00:00Z","timestamp":1715472000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Clinic Periodontology"],"published-print":{"date-parts":[[2024,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Aim<\/jats:title><jats:p>To investigate the association between periodontal macrophage polarization states and the alveolar bone levels, and to assess whether glycosylated nano\u2010hydroxyapatites (GHANPs) could improve bone regeneration in periodontitis by inducing macrophage M2 polarization.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and Methods<\/jats:title><jats:p>The change of macrophage polarization state in inflammatory periodontal tissues (with bone loss) was examined using clinical gingival samples. The relationship between macrophage phenotype and bone level in periodontal bone loss and repair was evaluated using a mouse periodontitis model. The effect of GHANPs on macrophage polarization was assessed by the in vitro model of lipopolysaccharide (LPS)\u2010stimulated inflammation. The polarization\u2010related markers were detected by immunofluorescence staining, real\u2010time polymerase chain reaction and enzyme\u2010linked immunosorbent assay analysis. The therapeutic effect of GHANPs on alveolar bone loss was explored in experimental periodontitis by histological staining and micro\u2010CT analysis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>A lower macrophage M2\/M1 ratio was observed in periodontitis\u2010affected human gingival tissues. The results of animal experiments demonstrated a positive correlation between a lower Arg\u20101\/iNOS ratio and accelerated alveolar bone loss; also, the proportion of Arg\u20101\u2010positive macrophages increased during bone repair and regeneration. The administration of GHANPs partially restored M2 macrophage polarization after LPS stimulation. GHANPs increased alveolar bone repair and regeneration in experimental periodontitis induced by ligation, potentially related to their macrophage M2 transition regulation.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The findings of this study indicate that the induction of macrophage M2 polarization can be considered a viable approach for enhancing inflammatory bone repair. Additionally, GHANPs show potential in the clinical treatment of periodontitis.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jcpe.13999","type":"journal-article","created":{"date-parts":[[2024,5,13]],"date-time":"2024-05-13T04:19:21Z","timestamp":1715573961000},"page":"1054-1065","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Polarized <scp>M2<\/scp> macrophages induced by glycosylated nano\u2010hydroxyapatites activate bone regeneration in periodontitis therapy"],"prefix":"10.1111","volume":"51","author":[{"given":"Dan","family":"Qiao","sequence":"first","affiliation":[{"name":"Nanjing Stomatological Hospital, Affiliated Hospital of Medical School, Institute of Stomatology Nanjing University  Nanjing People's Republic of China"},{"name":"Qinghai University Affiliated Hospital  Xining People's Republic of 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