{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T12:56:28Z","timestamp":1791896188032,"version":"4.3.4"},"reference-count":83,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2024,7,10]],"date-time":"2024-07-10T00:00:00Z","timestamp":1720569600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004587","name":"Instituto de Salud Carlos III","doi-asserted-by":"publisher","award":["PI21\/00588"],"award-info":[{"award-number":["PI21\/00588"]}],"id":[{"id":"10.13039\/501100004587","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Clinic Periodontology"],"published-print":{"date-parts":[[2024,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Aim<\/jats:title><jats:p>To identify new biomarkers to detect untreated and treated periodontitis in gingival crevicular fluid (GCF) using sequential window acquisition of all theoretical mass spectra (SWATH\u2010MS).<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and Methods<\/jats:title><jats:p>GCF samples were collected from 44 periodontally healthy subjects and 40 with periodontitis (Stages III\u2013IV). In the latter, 25 improved clinically 2\u2009months after treatment. Samples were analysed using SWATH\u2010MS, and proteins were identified by the UniProt human\u2010specific database. The diagnostic capability of the proteins was determined with generalized additive models to distinguish the three clinical conditions.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>In the untreated periodontitis <jats:italic>vs<\/jats:italic>. periodontal health modelling, five proteins showed excellent or good bias\u2010corrected (bc)\u2010sensitivity\/bc\u2010specificity values of &gt;80%. These were GAPDH, ZG16B, carbonic anhydrase 1, plasma protease inhibitor C1 and haemoglobin subunit beta. GAPDH with MMP\u20109, MMP\u20108, zinc\u2010\u03b1\u20102\u2010glycoprotein and neutrophil gelatinase\u2010associated lipocalin and ZG16B with cornulin provided increased bc\u2010sensitivity\/bc\u2010specificity of &gt;95%. For distinguishing treated periodontitis <jats:italic>vs<\/jats:italic>. periodontal health, most of these proteins and their combinations revealed a predictive ability similar to previous modelling. No model obtained relevant results to differentiate between periodontitis conditions.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>New single and dual GCF protein biomarkers showed outstanding results in discriminating untreated and treated periodontitis from periodontal health. Periodontitis conditions were indistinguishable. Future research must validate these findings.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jcpe.14037","type":"journal-article","created":{"date-parts":[[2024,7,11]],"date-time":"2024-07-11T03:01:27Z","timestamp":1720666887000},"page":"1342-1358","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Using <scp>SWATH<\/scp>\u2010<scp>MS<\/scp> to identify new molecular biomarkers in gingival crevicular fluid for detecting periodontitis and its response to treatment"],"prefix":"10.1111","volume":"51","author":[{"given":"T.","family":"Blanco\u2010Pintos","sequence":"first","affiliation":[{"name":"Oral Sciences Research Group, Special Needs Unit, Department of Surgery and Medical\u2010Surgical Specialties, School of Medicine and Dentistry, Universidade de Santiago de Compostela Health Research Institute of Santiago (IDIS)  Santiago de Compostela Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Regueira\u2010Iglesias","sequence":"additional","affiliation":[{"name":"Oral Sciences Research Group, Special Needs Unit, Department of Surgery and Medical\u2010Surgical Specialties, School of Medicine and Dentistry, Universidade de Santiago de Compostela Health Research Institute of Santiago (IDIS)  Santiago de Compostela Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Relvas","sequence":"additional","affiliation":[{"name":"Oral Pathology and Rehabilitation Research Unit (UNIPRO) University Institute of Health Sciences (IUCS\u2010CESPU)  Gandra Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Alonso\u2010Sampedro","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine and Clinical Epidemiology Complejo Hospitalario Universitario, Health Research Institute of Santiago (IDIS)  Santiago de Compostela Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. 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