{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,24]],"date-time":"2026-10-24T13:53:32Z","timestamp":1792850012288,"version":"4.5.0"},"reference-count":35,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2024,10,22]],"date-time":"2024-10-22T00:00:00Z","timestamp":1729555200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001459","name":"Ministry of Education - Singapore","doi-asserted-by":"publisher","award":["A\u20100002962\u201000\u201000"],"award-info":[{"award-number":["A\u20100002962\u201000\u201000"]}],"id":[{"id":"10.13039\/501100001459","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Clinic Periodontology"],"published-print":{"date-parts":[[2025,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Aims<\/jats:title>\n                    <jats:p>To determine the relationship between microbiome dysbiosis indices and biofilm immunogenicity and their prognostic implications on periodontal treatment response.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Materials and Methods<\/jats:title>\n                    <jats:p>Thirty periodontally healthy controls and 30 periodontitis cases (stage III) were recruited. Cases received non\u2010surgical periodontal therapy (NSPT), and their treatment response at 6\u2009months was evaluated using a treat\u2010to\u2010target endpoint (\u2264\u20094 sites with probing depths \u2265\u20095\u2009mm). Pooled subgingival biofilm samples were obtained from controls and cases. The V3\u20134 hypervariable region of the 16S rRNA gene was sequenced and two compositional indices (subgingival microbiome dysbiosis index, SMDI, and dysbiosis ratio, DR) were calculated. Nuclear factor kappa\u2010B (NF\u2010\u03baB) activation elicited by biofilm samples in monocytic reporter cells was quantified to assess biofilm immunogenicity.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      SMDI, DR and biofilm immunogenicity were highly diagnostic for periodontitis (area under curves [AUC] &gt;\u20090.90,\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.001). Among periodontitis cases, all three microbial parameters were significantly reduced after NSPT (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.001). Cases achieving the treat\u2010to\u2010target endpoint had lower pre\u2010treatment SMDI and biofilm immunogenicity (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.05) and different microbial recolonization patterns from poor responders. Both measures predicted treatment response (AUC of 0.767 and 0.835, respectively,\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.05).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Subgingival biofilm dysbiosis quantified using SMDI and biofilm immunogenicity was diagnostic of periodontitis and predictive of NSPT outcomes.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/jcpe.14082","type":"journal-article","created":{"date-parts":[[2024,10,23]],"date-time":"2024-10-23T02:53:50Z","timestamp":1729652030000},"page":"215-224","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Prognostic Impact of Microbiome Dysbiosis: A Prospective Study"],"prefix":"10.1111","volume":"52","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4407-0134","authenticated-orcid":false,"given":"Ren Jie Jacob","family":"Chew","sequence":"first","affiliation":[{"name":"Faculty of Dentistry National University of Singapore  Singapore Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2208-5397","authenticated-orcid":false,"given":"Charlene Enhui","family":"Goh","sequence":"additional","affiliation":[{"name":"Faculty of Dentistry National University of Singapore  Singapore Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-6284-1557","authenticated-orcid":false,"given":"Xin Yi Sheena","family":"Lin","sequence":"additional","affiliation":[{"name":"Faculty of Dentistry National University of Singapore  Singapore Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-8730-1668","authenticated-orcid":false,"given":"Feng Jun Bryan","family":"Oh","sequence":"additional","affiliation":[{"name":"Faculty of Dentistry National University of Singapore  Singapore Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-0804-1533","authenticated-orcid":false,"given":"Ruiqi Paul","family":"Sim","sequence":"additional","affiliation":[{"name":"Faculty of Dentistry National University of Singapore  Singapore Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7153-4865","authenticated-orcid":false,"given":"Philip M.","family":"Preshaw","sequence":"additional","affiliation":[{"name":"School of Dentistry University of Dundee  Dundee 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