{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T16:34:07Z","timestamp":1787762047010,"version":"build-2784847793"},"reference-count":45,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2024,11,12]],"date-time":"2024-11-12T00:00:00Z","timestamp":1731369600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Clinic Periodontology"],"published-print":{"date-parts":[[2025,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Aim<\/jats:title>\n                    <jats:p>To characterize the gingival crevicular fluid (GCF) and plasma extracellular vesicles (EVs) and explore their proteomic cargo in healthy pregnant women compared to those with gestational diabetes mellitus (GDM) and periodontitis.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>One\u2010hundred and four pregnant women were recruited at 24\u201330 gestation weeks. GDM was diagnosed by an oral glucose tolerance test. GCF and plasma samples were obtained to isolate EVs and characterized by nanoparticle tracking, immunoassays, electron microscopy and mass spectrometry.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Of the recruits,17.3% women were healthy, 50% had periodontitis and 32.7% had both GDM and periodontitis. Probing depth, clinical attachment loss and bleeding on probing were more severe in GDM and periodontitis pregnancies (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.0001). Additionally, this group showed an increase concentration of total, small and large GCF\u2010EVs (\n                      <jats:italic>p\u2009=<\/jats:italic>\n                      \u20090.0015,\n                      <jats:italic>p\u2009=<\/jats:italic>\n                      \u20090.0011 and\n                      <jats:italic>p\u2009=<\/jats:italic>\n                      \u20090.0008, respectively), with decreased expression of CD9, CD81 and CD81\/CD63 ratio (\n                      <jats:italic>p\u2009=<\/jats:italic>\n                      \u20090.0461,\n                      <jats:italic>p\u2009=<\/jats:italic>\n                      \u20090.0164 and\n                      <jats:italic>p\u2009=<\/jats:italic>\n                      \u20090.0005, respectively). No differences were observed in plasmatic EVs concentration or markers expression. Proteomic analysis of GCF\u2010EVs showed peptides of both host and bacterial origin. Gene ontology analysis revealed that proteins of GCF\u2010EVs participate in immune inflammatory responses, glucose metabolism and insulin response mechanisms.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>GCF\u2010EVs were increased in both GDM and periodontitis, and their proteomic cargo suggest their involvement in immune inflammatory response, glucose metabolism and insulin pathways during pregnancy.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/jcpe.14083","type":"journal-article","created":{"date-parts":[[2024,11,13]],"date-time":"2024-11-13T06:59:10Z","timestamp":1731481150000},"page":"225-236","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Proteomic Insights Into Gingival Crevicular Extracellular Vesicles in Periodontitis and Gestational Diabetes: An Exploratory Study"],"prefix":"10.1111","volume":"52","author":[{"given":"Mar\u00eda Luisa","family":"Mizgier","sequence":"first","affiliation":[{"name":"Department of Oral Pathology and Conservative Dentistry, Periodontics, Faculty of Dentistry Universidad de Los Andes  Santiago Chile"},{"name":"Center for Biomedical Research and Innovation (CIIB), Universidad de Los Andes  Santiago Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gino","family":"Nardocci","sequence":"additional","affiliation":[{"name":"Center for Biomedical Research and Innovation (CIIB), Universidad de Los Andes  Santiago Chile"},{"name":"Faculty of Medicine, School of Medicine Universidad de Los Andes  Santiago Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Valeria","family":"Ram\u00edrez","sequence":"additional","affiliation":[{"name":"Department of Statistics and Epidemiology, Faculty of Dentistry Universidad de Los Andes  Santiago Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mar\u00eda Jos\u00e9","family":"Bendek","sequence":"additional","affiliation":[{"name":"Department of Oral Pathology and Conservative Dentistry, Periodontics, Faculty of Dentistry Universidad de Los Andes  Santiago Chile"},{"name":"Center for Biomedical Research and Innovation (CIIB), Universidad de Los Andes  Santiago Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcela","family":"Hern\u00e1ndez","sequence":"additional","affiliation":[{"name":"Laboratory of Periodontal Biology and Department of Pathology and Oral Medicine, Faculty of Dentistry University of Chile  Santiago Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carolina","family":"Rojas","sequence":"additional","affiliation":[{"name":"Department of Oral Pathology and Conservative Dentistry, Periodontics, Faculty of Dentistry Universidad de Los Andes  Santiago Chile"},{"name":"Center for Biomedical Research and Innovation (CIIB), Universidad de Los Andes  Santiago Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Herrera","sequence":"additional","affiliation":[{"name":"ETEP (Etiology and Therapy of Periodontal and Peri\u2010Implant Diseases) Research Group University Complutense  Madrid Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alpdogan","family":"Kantarci","sequence":"additional","affiliation":[{"name":"Forsyth Institute  Cambridge Massachusetts USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthew W.","family":"Kemp","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynaecology, Yong Loo Lin School of Medicine National University of Singapore  Singapore Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sebasti\u00e1n E.","family":"Illanes","sequence":"additional","affiliation":[{"name":"Center for Biomedical Research and Innovation (CIIB), 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