{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T16:30:35Z","timestamp":1787761835572,"version":"build-2784847793"},"reference-count":72,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2024,12,18]],"date-time":"2024-12-18T00:00:00Z","timestamp":1734480000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100020222","name":"AAID Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100020222","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100001440","name":"ADA Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100001440","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Clinic Periodontology"],"published-print":{"date-parts":[[2025,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:title>Aims<\/jats:title><jats:p>This narrative review aims to synthesize current knowledge on integrating single\u2010cell genomics technologies with animal models of periodontitis and peri\u2010implantitis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Review<\/jats:title><jats:p>Single\u2010cell RNA sequencing (scRNAseq) reveals cellular heterogeneity and specific cell roles in periodontitis and peri\u2010implantitis, overcoming the limitations of bulk RNA sequencing. Under controlled conditions and genetic manipulation, animal models facilitate studying disease progression, gene functions and systemic disease links, aiding targeted therapy development. Knockout models have started to elucidate the impact of genetic mutations on periodontal disease and host responses. scRNAseq in animal models has been used to examine connections between periodontitis and systemic diseases, revealing altered immune environments and cellular interactions. Emerging studies are now applying these methods to animal models of peri\u2010implantitis. Integrating these datasets into single\u2010cell and spatially resolved atlases will enable future meta\u2010analyses, providing deeper insights into disease mechanisms considering factors such as sex, strain, and age.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Integrating scRNAseq with animal models advances the understanding of periodontitis and peri\u2010implantitis pathogenesis and precision therapies. The combined use of single\u2010cell and spatial genomics and scRNAseq will further enhance data insights significantly for drug discovery and preclinical testing, making these technologies pivotal in validating animal models and translating findings into clinical practice.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jcpe.14093","type":"journal-article","created":{"date-parts":[[2024,12,19]],"date-time":"2024-12-19T04:48:31Z","timestamp":1734583711000},"page":"268-279","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Application of Single\u2010Cell Genomics to Animal Models of\u00a0Periodontitis and Peri\u2010Implantitis"],"prefix":"10.1111","volume":"52","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5150-2076","authenticated-orcid":false,"given":"Akira","family":"Hasuike","sequence":"first","affiliation":[{"name":"Lab of Oral &amp; Craniofacial Innovation (LOCI), Department of Innovation &amp; Technology Research 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