{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T01:08:11Z","timestamp":1787015291847,"version":"build-2736575974"},"reference-count":62,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2025,1,8]],"date-time":"2025-01-08T00:00:00Z","timestamp":1736294400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100008108","name":"Taipei Tzu Chi Hospital","doi-asserted-by":"publisher","award":["TCRD\u2010TPE\u2010112\u201008"],"award-info":[{"award-number":["TCRD\u2010TPE\u2010112\u201008"]}],"id":[{"id":"10.13039\/501100008108","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Clinic Periodontology"],"published-print":{"date-parts":[[2025,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Aim<\/jats:title>\n                    <jats:p>Neurodegenerative diseases are characterized by early increased beta\u2010amyloid (A\u03b2) and decreased cerebrovascular reactivity. We investigated A\u03b2 in gingiva, serum or hippocampus and neurovascular reactivity in basilar artery (BA) of periodontitis rats, to test the impact of A\u03b2 on BA vasoreactivity ex\u00a0vivo.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Materials and Methods<\/jats:title>\n                    <jats:p>Periodontitis was induced in 32 rats using silk\u2010ligation. Rats were sacrificed at weeks 0, 1, 2 and 4. Gingival TNF\u2010\u03b1, IL\u20101\u03b2 and A\u03b2 were quantified via immunoblotting. Alveolar bone destruction was examined by micro\u2010computed tomography. Serum and hippocampus A\u03b2 values were measured by enzyme\u2010linked immunosorbent assay and fluorescence staining, respectively. Vasoreactivity was measured by myography on isolated BA.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>From Week 1, gingival TNF\u2010\u03b1 and IL\u20101\u03b2 and bone destruction increased. Gingiva, serum and hippocampus A\u03b2 values increased from Week 2. Nicotine\u2010induced BA relaxation declined from Week 2, while acetylcholine\u2010induced relaxation decreased by Week 4. Bone loss correlated with A\u03b2 and nicotine\u2010induced relaxation. Correlations were observed between A\u03b2s in tissues, between two induced BA relaxations and between A\u03b2 expressions and the induced relaxations. Ex\u00a0vivo, A\u03b2 reduced nicotine\u2010 and isoproterenol\u2010induced relaxations but not electrical stimulated relaxation.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Periodontitis may increase A\u03b2 expressions and reduce BA neurovascular reactivity, with A\u03b2 contributing to this abnormal neurovascular coupling.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/jcpe.14110","type":"journal-article","created":{"date-parts":[[2025,1,9]],"date-time":"2025-01-09T00:35:06Z","timestamp":1736382906000},"page":"762-772","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Periodontitis Increases Gingival, Serum and Hippocampus \u03b2\u2010Amyloid Expressions but Reduces Neurovascular Coupling in Basilar Artery of Rats"],"prefix":"10.1111","volume":"52","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0637-0208","authenticated-orcid":false,"given":"Earl","family":"Fu","sequence":"first","affiliation":[{"name":"Department of Dentistry Taipei Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation  New Taipei City Taiwan, ROC"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kuo\u2010Feng","family":"Huang","sequence":"additional","affiliation":[{"name":"Division of Neurosurgery, Department of Surgery Taipei Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation  New Taipei City Taiwan, ROC"},{"name":"School of Medicine Tzu Chi University  Hualien Taiwan, ROC"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hsi\u2010Hsien","family":"Chang","sequence":"additional","affiliation":[{"name":"Division of Urology, Department of Surgery Taipei Tzu Chi Hospital, Buddhist Medical Foundation  New Taipei Taiwan, ROC"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hui\u2010Hwa","family":"Tseng","sequence":"additional","affiliation":[{"name":"Department of Pathology Taipei Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation  New Taipei City Taiwan, ROC"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephen Shei\u2010Dei","family":"Yang","sequence":"additional","affiliation":[{"name":"School of Medicine Tzu Chi University  Hualien Taiwan, ROC"},{"name":"Division of Urology, Department of Surgery Taipei Tzu Chi Hospital, Buddhist Medical Foundation  New Taipei Taiwan, ROC"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,1,8]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/s0006\u20108993(03)02632\u20105"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600\u20100528.2011.00650.x"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1093\/brain\/awv313"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1177\/0271678X20982383"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bj.2018.12.001"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.01016.2011"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13195\u2010017\u20100282\u20106"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.3233\/JAD\u2010215317"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/micc.12686"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.3390\/brainsci10100679"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006\u20108993(91)90870\u20102"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/prd.12344"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1001\/jamaneurol.2014.1646"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600\u2010051X.2007.01172.x"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12974\u2010021\u201002071\u20109"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1097\/FJC.0000000000000838"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2017.07.030"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0151081"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0204941"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.31887\/DCNS.2013.15.4\/hjahn"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1532\u20105415.2010.02788.x"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014\u20102999(91)90618\u2010z"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.archoralbio.2014.03.004"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1748\u20101716.2010.02231.x"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.archoralbio.2019.01.008"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1111\/micc.12858"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1124\/jpet.302.1.397"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.18632\/aging.103306"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.3233\/JAD\u2010181268"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pnpbp.2005.08.007"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1155\/2014\/476068"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1111\/cdoe.12128"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.3233\/JAD\u2010190298"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.2001.281.6.H2417"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuint.2022.105282"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1038\/nn.2583"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.3233\/JAD\u2010121918"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.3233\/JAD\u2010201007"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbc.2021.100402"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.atherosclerosis.2015.04.813"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmhi.2014.01.001"},{"key":"e_1_2_9_43_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1074069"},{"key":"e_1_2_9_44_1","doi-asserted-by":"publisher","DOI":"10.1101\/cshperspect.a004457"},{"key":"e_1_2_9_45_1","doi-asserted-by":"publisher","DOI":"10.1111\/jre.13224"},{"key":"e_1_2_9_46_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.res.0000024417.79275.23"},{"key":"e_1_2_9_47_1","doi-asserted-by":"publisher","DOI":"10.1124\/jpet.102.046854"},{"key":"e_1_2_9_48_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1748\u20101716.2010.02224.x"},{"key":"e_1_2_9_49_1","doi-asserted-by":"publisher","DOI":"10.1177\/0271678X17732449"},{"key":"e_1_2_9_50_1","doi-asserted-by":"publisher","DOI":"10.1038\/35086072"},{"key":"e_1_2_9_51_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.archoralbio.2021.105313"},{"key":"e_1_2_9_52_1","doi-asserted-by":"publisher","DOI":"10.3390\/microorganisms11071832"},{"key":"e_1_2_9_53_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00415\u2010016\u20108234\u20109"},{"key":"e_1_2_9_54_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.10\u2010156661"},{"key":"e_1_2_9_55_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M111.244335"},{"key":"e_1_2_9_56_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.275.8.5626"},{"key":"e_1_2_9_57_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.01021.2020"},{"key":"e_1_2_9_58_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.dentre.2022.100062"},{"key":"e_1_2_9_59_1","doi-asserted-by":"publisher","DOI":"10.1111\/jre.12600"},{"key":"e_1_2_9_60_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbi.2017.06.002"},{"key":"e_1_2_9_61_1","doi-asserted-by":"publisher","DOI":"10.1111\/jnc.15096"},{"key":"e_1_2_9_62_1","doi-asserted-by":"publisher","DOI":"10.26502\/fjppr.070"},{"key":"e_1_2_9_63_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-3565(24)37261-1"}],"container-title":["Journal of Clinical Periodontology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jcpe.14110","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,11,8]],"date-time":"2025-11-08T02:51:59Z","timestamp":1762570319000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/jcpe.14110"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,1,8]]},"references-count":62,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2025,5]]}},"alternative-id":["10.1111\/jcpe.14110"],"URL":"https:\/\/doi.org\/10.1111\/jcpe.14110","archive":["Portico"],"relation":{},"ISSN":["0303-6979","1600-051X"],"issn-type":[{"value":"0303-6979","type":"print"},{"value":"1600-051X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,1,8]]},"assertion":[{"value":"2024-08-13","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-12-15","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-01-08","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}