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Drp1 tetramer formation and phosphorylation were analysed using western blot. THP\u20101 macrophages and RAW264.7 cells were stimulated with\n                      <jats:styled-content style=\"fixed-case\">\n                        <jats:italic>Porphyromonas gingivalis<\/jats:italic>\n                      <\/jats:styled-content>\n                      (\n                      <jats:italic>Pg<\/jats:italic>\n                      ) or\n                      <jats:italic>Pg<\/jats:italic>\n                      lipopolysaccharide (\n                      <jats:italic>Pg<\/jats:italic>\n                      LPS), respectively. Alterations in proteins associated with mitochondrial dynamics were scrutinized via western blot. Immunofluorescence was used to evaluate mitochondrial damage and mitochondrial permeability transition pore (mPTP) opening. Western blot was used to examine the inflammatory markers NLRP3, caspase\u20101, IL\u20101\u03b2 and GSDMD. Protein interactions involving Drp1 were verified through immunoprecipitation.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      In periodontitis patient samples,\n                      <jats:italic>Pg<\/jats:italic>\n                      LPS\u2010treated RAW264.7 cells, and\n                      <jats:italic>Pg<\/jats:italic>\n                      \u2010stimulated THP\u20101 macrophages, over\u2010activated Drp1 was able to drive NLRP3 inflammasome activation and the subsequent release of inflammatory factors. A direct interaction between Drp1 and HK1 was observed, facilitating excessive mPTP opening and subsequent mitochondrial dysfunction.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>In the inflammatory milieu of periodontal tissues, Drp1 hyperactivation in the macrophages is implicated in inflammation induction. Modulation of the inflammatory response in periodontal macrophages by Drp1 appears to facilitate mPTP opening.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/jcpe.14111","type":"journal-article","created":{"date-parts":[[2025,1,6]],"date-time":"2025-01-06T22:54:47Z","timestamp":1736204087000},"page":"622-636","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Dynamin\u2010Related Protein 1 Orchestrates Inflammatory Responses in Periodontal Macrophages via Interaction With\u00a0Hexokinase 1"],"prefix":"10.1111","volume":"52","author":[{"given":"Yiming","family":"Jiang","sequence":"first","affiliation":[{"name":"The VIP Department, School and Hospital of Stomatology China Medical University, Liaoning Provincial Key Laboratory of Oral Diseases  Shenyang 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