{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,14]],"date-time":"2026-09-14T20:05:34Z","timestamp":1789416334948,"version":"build-2803163510"},"reference-count":50,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2025,1,21]],"date-time":"2025-01-21T00:00:00Z","timestamp":1737417600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,1,21]],"date-time":"2025-01-21T00:00:00Z","timestamp":1737417600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82101061"],"award-info":[{"award-number":["82101061"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Clinic Periodontology"],"published-print":{"date-parts":[[2025,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Aim<\/jats:title>\n                    <jats:p>To investigate the involvement of low\u2010density lipoprotein receptor\u2010related protein 5 (LRP5) in inflammation and alveolar bone loss in periodontitis.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Materials and Methods<\/jats:title>\n                    <jats:p>\n                      Gingival tissues were obtained from 10 periodontitis patients and 10 healthy individuals. Wild\u2010type (WT) and osteoblast\u2010specific\n                      <jats:italic>Lrp5<\/jats:italic>\n                      conditional knock\u2010out C57BL\/6 (LRP5\n                      <jats:sup>fl\/fl<\/jats:sup>\n                      ;Oc\u2010Cre) mice were used to establish a ligature\u2010induced mouse model of periodontitis. Human periodontal ligament stem cells (hPDLSCs) were isolated and used to further verify the mechanism through which LRP5 mediates periodontitis in\u00a0vitro. Micro\u2010computed tomography, haematoxylin and eosin staining, immunohistochemistry, quantitative reverse transcription PCR, western blotting, enzyme\u2010linked immunosorbent assay and RNA sequencing were performed to explore the role of LRP5 in periodontitis and the underlying mechanism.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      LRP5 expression was down\u2010regulated in human\/mouse periodontal tissues compared to that in healthy controls. Compared to those in wild\u2010type mice, the periodontal tissues of LRP5\n                      <jats:sup>fl\/fl<\/jats:sup>\n                      ;Oc\u2010Cre mice had increased alveolar bone loss, higher proinflammatory cytokine levels, and lower osteogenesis\u2010related factor expression. LRP5 expression was down\u2010regulated in hPDLSCs after lipopolysaccharide treatment in\u00a0vitro.\n                      <jats:italic>LRP5<\/jats:italic>\n                      knockdown increased proinflammatory cytokine production and inhibited osteoblastogenesis by inhibiting PI3K\/c\u2010FOS signalling.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>LRP5 down\u2010regulation exacerbates inflammation and alveolar bone loss in periodontitis by inhibiting PI3K\/c\u2010FOS signalling, suggesting LRP5 as a potential therapeutic target for periodontitis.<\/jats:p>\n                  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