{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T03:13:30Z","timestamp":1777432410691,"version":"3.51.4"},"reference-count":42,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2025,6,3]],"date-time":"2025-06-03T00:00:00Z","timestamp":1748908800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100005847","name":"Health and Medical Research Fund","doi-asserted-by":"publisher","award":["07182796"],"award-info":[{"award-number":["07182796"]}],"id":[{"id":"10.13039\/501100005847","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Science Foundation of China","doi-asserted-by":"publisher","award":["82301157"],"award-info":[{"award-number":["82301157"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Clinic Periodontology"],"published-print":{"date-parts":[[2025,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>The diagnostic performance of active matrix metalloproteinase\u20108 (aMMP\u20108) point\u2010of\u2010care oral rinse test (POC\u2010ORT) for periodontitis needs to be improved because of its relatively low sensitivity. This study attempted to identify the clinical features associated with the misclassification of periodontal cases by the test.<\/jats:p><\/jats:sec><jats:sec><jats:title>Method<\/jats:title><jats:p>This work consisted of two cross\u2010sectional diagnostic accuracy studies involving a representative cohort in Hong Kong, China, and a convenience sample of subjects in Shanghai, China. The outcomes of the aMMP\u20108 POC\u2010ORT (index test) were compared to the case definitions of the 2017 World Workshop on the classification of periodontal health status (reference test). The analysis reports the diagnostic accuracy parameters, the Youden index and correlations between the aMMP\u20108 test outcomes and clinical features.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>In this study, 384 and 390 subjects were enrolled in Hong Kong and Shanghai, respectively. The conventional 20\u2009ng\/mL threshold failed to detect more than 50% of early\u2010stage (I\/II) cases. The positive POC\u2010ORT results were significantly correlated with the number of sites with bleeding on probing (BOP), probing pocket depth (PPD) \u2265\u20094\u2009mm, PPD \u2265\u20095\u2009mm, PPD \u2265\u20094\u2009mm and BOP, PPD \u2265\u20095\u2009mm and BOP and clinical attachment loss. The adjusted odds ratio (OR) for a positive test increased with the number of sites with PPD \u2265\u20095\u2009mm: 1.092 (95% CI: 1.063\u20131.127, <jats:italic>p<\/jats:italic>\u2009&lt;\u20090.001) for the Hong Kong sample and 1.074 (95% CI: 1.050\u20131.100, <jats:italic>p<\/jats:italic>\u2009&lt;\u20090.001) for the Shanghai sample, suggesting a higher likelihood of a positive result with each additional pocket \u2265\u20095\u2009mm. The same was observed for PPD \u2265\u20095\u2009mm with BOP: 1.093 (95% CI: 1.064\u20131.129, <jats:italic>p<\/jats:italic>\u2009&lt;\u20090.001) and 1.077 (95% CI: 1.052\u20131.105, <jats:italic>p<\/jats:italic>\u2009&lt;\u20090.001). Notably, the false\u2010negative cases were characterised by a significantly smaller number of periodontal pockets, BOP percentages and bleeding pockets than the true\u2010positive ones.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The false\u2010negative cases, which are responsible for the observed low sensitivity of the POC\u2010ORT, showed more\u00a0localised periodontal inflammation and pocketing. As the oral rinse test measures the overall level of aMMP\u20108 cleared by the gingival crevicular fluid from various lesions throughout the whole dentition, localised periodontitis appears more\u00a0difficult to detect. Additional studies are needed to optimise the sampling strategy and set the cut\u2010off value of a positive\u00a0test.<\/jats:p><jats:p>\n<jats:bold>Trial Registration:<\/jats:bold> The study was approved by the Institutional Review Board of the University of Hong Kong\/Hospital Authority Hong Kong West Cluster (IRB Approval: UW22\u2010132; trial registration: NCT03928080) and the Shanghai Ninth People's Hospital Ethics Committee (IRB Approval: SH9H\u20102021\u2010T408\u20103; trial registration: NCT05513599)<\/jats:p><\/jats:sec>","DOI":"10.1111\/jcpe.14189","type":"journal-article","created":{"date-parts":[[2025,6,12]],"date-time":"2025-06-12T10:57:49Z","timestamp":1749725869000},"page":"1276-1287","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Clinical Features Associated With Periodontal Case Misclassification by an Active Matrix Metalloproteinase\u20108 Point\u2010of\u2010Care Oral Rinse Test"],"prefix":"10.1111","volume":"52","author":[{"given":"Mengning","family":"Bi","sequence":"first","affiliation":[{"name":"Shanghai Perio\u2010Implant Innovation Center Institute of Integrated Oral, Craniofacial and Sensory Research, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yu","family":"Xie","sequence":"additional","affiliation":[{"name":"Shanghai Perio\u2010Implant Innovation Center Institute of Integrated Oral, Craniofacial and Sensory Research, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaoyu","family":"Yu","sequence":"additional","affiliation":[{"name":"Shanghai Perio\u2010Implant Innovation Center Institute of Integrated Oral, Craniofacial and Sensory Research, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hairui","family":"Li","sequence":"additional","affiliation":[{"name":"Shanghai Perio\u2010Implant Innovation Center Institute of Integrated Oral, Craniofacial and Sensory Research, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1917-1988","authenticated-orcid":false,"given":"George","family":"Pelekos","sequence":"additional","affiliation":[{"name":"Division of Periodontology and Implant Dentistry, Faculty of Dentistry The University of Hong Kong  Hong Kong SAR China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lijian","family":"Jin","sequence":"additional","affiliation":[{"name":"Division of Periodontology and Implant Dentistry, Faculty of Dentistry The University of Hong Kong  Hong Kong SAR 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