{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,2]],"date-time":"2026-06-02T22:37:41Z","timestamp":1780439861422,"version":"3.54.1"},"reference-count":59,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2025,6,24]],"date-time":"2025-06-24T00:00:00Z","timestamp":1750723200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100008040","name":"Kao Corporation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100008040","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Clinic Periodontology"],"published-print":{"date-parts":[[2025,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:title>Aim<\/jats:title><jats:p>To elucidate the characteristics of the subgingival plaque microbiome in older adults without gingival inflammation.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and Methods<\/jats:title><jats:p>Subgingival plaque from 180 participants was collected and analysed using 16S rRNA sequencing. Based on the clinical parameters at the sampling sites, participants were categorised as healthy (gingival index [GI] =\u20090, maximum probing pocket depth [PPD<jats:sub>max<\/jats:sub>] \u2264\u20092.0 and gingival recession [GR] =\u20090) or non\u2010healthy (GI &gt;\u20090, or PPD<jats:sub>max<\/jats:sub> &gt;\u20092.0 or GR &gt;\u20090). Each group was further stratified by age into younger (&lt;\u200965\u2009years) and older (\u2265\u200965\u2009years) subgroups. We performed diversity and linear discriminant effect size (LEfSe) analyses to elucidate microbiome characteristics of healthy older adults.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>We observed differences in \u03b1\u2010diversity and \u03b2\u2010diversity between younger and older individuals only in the healthy group. Healthy older individuals showed a lower \u03b1\u2010diversity index, indicating a healthy\u2010like profile shift and also a significantly greater difference in \u03b2\u2010diversity from the non\u2010healthy group than the healthy younger subgroup. LEfSe analysis indicated that six amplicon sequence variants (ASVs), such as <jats:styled-content style=\"fixed-case\">\n<jats:italic>Rothia dentocariosa<\/jats:italic>\n<\/jats:styled-content>, <jats:styled-content style=\"fixed-case\">\n<jats:italic>Neisseria perflava<\/jats:italic>\n<\/jats:styled-content> and <jats:italic>Actinomyces<\/jats:italic> sp. HMT\u2010448, were predominant in the healthy older subgroup.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Maintaining lower \u03b1\u2010diversity, with an abundance of <jats:styled-content style=\"fixed-case\">\n<jats:italic>R. dentocariosa<\/jats:italic>\n<\/jats:styled-content> and <jats:italic>\n<jats:styled-content style=\"fixed-case\">N. perflava<\/jats:styled-content>,<\/jats:italic> which are possible nitrate\u2010reducing bacteria, may contribute to lifelong healthy gingiva by preventing microbial dysbiosis.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jcpe.14192","type":"journal-article","created":{"date-parts":[[2025,6,24]],"date-time":"2025-06-24T20:44:42Z","timestamp":1750797882000},"page":"1314-1326","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Characteristics of Subgingival Plaque Microbiome in Japanese Older Adults With Healthy Gingiva"],"prefix":"10.1111","volume":"52","author":[{"ORCID":"https:\/\/orcid.org\/0009-0001-6248-2207","authenticated-orcid":false,"given":"Tomoki","family":"Akatsu","sequence":"first","affiliation":[{"name":"Biological Science Research Kao Corporation  Haga\u2010gun Tochigi Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hatsumi","family":"Souno","sequence":"additional","affiliation":[{"name":"Biological Science Research Kao Corporation  Haga\u2010gun Tochigi Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3825-5263","authenticated-orcid":false,"given":"Akihiko","family":"Fujii","sequence":"additional","affiliation":[{"name":"Biological Science Research Kao Corporation  Haga\u2010gun Tochigi Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoshihiko","family":"Minegishi","sequence":"additional","affiliation":[{"name":"Biological Science Research Kao Corporation  Haga\u2010gun Tochigi Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Noriyasu","family":"Ota","sequence":"additional","affiliation":[{"name":"Biological Science Research Kao Corporation  Haga\u2010gun Tochigi Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoshihisa","family":"Yamashita","sequence":"additional","affiliation":[{"name":"Division of Infectious and Molecular Biology Kyushu Dental University  Kitakyushu Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,6,24]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/ismej.2012.174"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/prd.12362"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cden.2005.03.003"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1080\/10408398.2024.2351168"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/jcpe.13827"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1177\/0022034514529974"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/prd.12473"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/jcpe.12944"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/jcpe.12087"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41587\u2010019\u20100209\u20109"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.3869"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41396\u2010017\u20100037\u20101"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro2337"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1186\/gb\u20102014\u201015\u20107\u2010r89"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/prd.12135"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.1128\/mSystems.00187\u201018"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/prd.12136"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.1111\/jcpe.12677"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2017.02224"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.1161\/JAHA.119.013324"},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.1038\/ismej.2011.191"},{"key":"e_1_2_11_23_1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600\u2010051x.2001.028005377.x"},{"key":"e_1_2_11_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.job.2013.09.001"},{"key":"e_1_2_11_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2023.02.009"},{"key":"e_1_2_11_26_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0127077"},{"key":"e_1_2_11_27_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0088645"},{"key":"e_1_2_11_28_1","doi-asserted-by":"publisher","DOI":"10.1111\/jcpe.13793"},{"key":"e_1_2_11_29_1","doi-asserted-by":"publisher","DOI":"10.1111\/jcpe.12542"},{"key":"e_1_2_11_30_1","doi-asserted-by":"publisher","DOI":"10.1002\/JPER.20\u20100778"},{"key":"e_1_2_11_31_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.02712\u201014"},{"key":"e_1_2_11_32_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010022\u201010559\u20104"},{"key":"e_1_2_11_33_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.05371\u201011"},{"key":"e_1_2_11_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molmed.2014.11.004"},{"key":"e_1_2_11_35_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2021.629723"},{"key":"e_1_2_11_36_1","doi-asserted-by":"publisher","DOI":"10.1038\/ismej.2014.28"},{"key":"e_1_2_11_37_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0189374"},{"key":"e_1_2_11_38_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.1967.38.6.610"},{"key":"e_1_2_11_39_1","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.26082\u20100"},{"key":"e_1_2_11_40_1","doi-asserted-by":"publisher","DOI":"10.1038\/ismej.2014.114"},{"key":"e_1_2_11_41_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41522\u2010023\u201000406\u20103"},{"key":"e_1_2_11_42_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0061217"},{"key":"e_1_2_11_43_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41592\u2010019\u20100616\u20103"},{"key":"e_1_2_11_44_1","doi-asserted-by":"publisher","DOI":"10.3390\/mps6040071"},{"key":"e_1_2_11_45_1","doi-asserted-by":"publisher","DOI":"10.4103\/0972\u2010124X.118334"},{"key":"e_1_2_11_46_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140\u20106736(05)67728\u20108"},{"key":"e_1_2_11_47_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010020\u201069931\u2010x"},{"key":"e_1_2_11_48_1","doi-asserted-by":"publisher","DOI":"10.1177\/00220345221080982"},{"key":"e_1_2_11_49_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41368\u2010023\u201000266\u20109"},{"key":"e_1_2_11_50_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2020.555465"},{"key":"e_1_2_11_51_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2020.604596"},{"key":"e_1_2_11_52_1","doi-asserted-by":"publisher","DOI":"10.1186\/gb\u20102011\u201012\u20106\u2010r60"},{"key":"e_1_2_11_53_1","doi-asserted-by":"publisher","DOI":"10.1111\/omi.12479"},{"key":"e_1_2_11_54_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600\u2010051x.1998.tb02419.x"},{"key":"e_1_2_11_55_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep22164"},{"key":"e_1_2_11_56_1","doi-asserted-by":"publisher","DOI":"10.3945\/ajcn.115.116244"},{"key":"e_1_2_11_57_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2019.00053"},{"key":"e_1_2_11_58_1","doi-asserted-by":"publisher","DOI":"10.1111\/jcpe.13819"},{"key":"e_1_2_11_59_1","doi-asserted-by":"publisher","DOI":"10.1128\/mSphere.00558\u201019"},{"key":"e_1_2_11_60_1","doi-asserted-by":"publisher","DOI":"10.1111\/1462\u20102920.12502"}],"container-title":["Journal of Clinical Periodontology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jcpe.14192","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,8,25]],"date-time":"2025-08-25T23:31:29Z","timestamp":1756164689000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/jcpe.14192"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,6,24]]},"references-count":59,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2025,9]]}},"alternative-id":["10.1111\/jcpe.14192"],"URL":"https:\/\/doi.org\/10.1111\/jcpe.14192","archive":["Portico"],"relation":{},"ISSN":["0303-6979","1600-051X"],"issn-type":[{"value":"0303-6979","type":"print"},{"value":"1600-051X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,6,24]]},"assertion":[{"value":"2024-07-11","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-05-26","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-06-24","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}