{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T01:35:30Z","timestamp":1791941730492,"version":"4.3.4"},"reference-count":45,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2025,10,12]],"date-time":"2025-10-12T00:00:00Z","timestamp":1760227200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2025,10,12]],"date-time":"2025-10-12T00:00:00Z","timestamp":1760227200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100009252","name":"Science for Life Laboratory","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100009252","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100015701","name":"Uppsala Multidisciplinary Center for Advanced Computational Science","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100015701","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Clinic Periodontology"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Aim<\/jats:title>\n                    <jats:p>To analyse gene expression profiles in human tissue samples obtained from dental implant sites with or without peri\u2010implantitis.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Materials and Methods<\/jats:title>\n                    <jats:p>\n                      Soft\u2010tissue biopsy samples obtained from patients presenting with implants with severe peri\u2010implantitis and adjacent reference implants with no signs of peri\u2010implantitis were analysed by spatial transcriptomics (\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u20094), RNA\u2010sequencing (\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u200920) and immunohistochemistry (\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u200938).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>The analysis disclosed evident associations between distinct gene clusters and specific tissue compartments in both groups of specimens. Samples from peri\u2010implantitis sites showed higher levels of gene activity than samples from reference implant sites. Gene\u2010set enrichment analysis revealed that several important biological pathways, for example, \u2018regulation of antimicrobial host response\u2019, \u2018collagen fibril organisation\u2019 and \u2018regulation of angiogenesis\u2019, were severely dysregulated in peri\u2010implantitis lesions when compared to reference implant sites. Similarly, immunohistochemical evaluation showed elevated levels of proteins corresponding to the most up\u2010regulated, differentially expressed genes (DEGs), which were identified by spatial transcriptomics and RNA\u2010sequencing.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Peri\u2010implant lesions demonstrated associations between gene clusters and specific tissue compartments. Several biological pathways linked to the activation of host response towards bacterial insults were dysregulated in peri\u2010implantitis specimens when compared to reference implant samples.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/jcpe.70008","type":"journal-article","created":{"date-parts":[[2025,10,12]],"date-time":"2025-10-12T23:55:50Z","timestamp":1760313350000},"page":"137-151","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Spatial Transcriptomic Assessments of Gene Expression Profiles in Human Peri\u2010Implant Lesions"],"prefix":"10.1111","volume":"53","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4195-0729","authenticated-orcid":false,"given":"C.","family":"Dionigi","sequence":"first","affiliation":[{"name":"Department of Periodontology Institute of Odontology, Sahlgrenska Academy, University of Gothenburg  Gothenburg Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A. 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