{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,30]],"date-time":"2026-10-30T07:32:31Z","timestamp":1793345551927,"version":"4.5.0"},"reference-count":48,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2025,10,2]],"date-time":"2025-10-02T00:00:00Z","timestamp":1759363200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2025,10,2]],"date-time":"2025-10-02T00:00:00Z","timestamp":1759363200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["2019\/09740\u20103"],"award-info":[{"award-number":["2019\/09740\u20103"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","award":["001"],"award-info":[{"award-number":["001"]}],"id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","award":["88887.717561\/2022\u201000"],"award-info":[{"award-number":["88887.717561\/2022\u201000"]}],"id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100010763","name":"Sveriges Ornitologiska F\u00f6rening","doi-asserted-by":"publisher","award":["FoUI\u2010966140"],"award-info":[{"award-number":["FoUI\u2010966140"]}],"id":[{"id":"10.13039\/100010763","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100010763","name":"Sveriges Ornitologiska F\u00f6rening","doi-asserted-by":"publisher","award":["FoUI\u2010978687"],"award-info":[{"award-number":["FoUI\u2010978687"]}],"id":[{"id":"10.13039\/100010763","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Clinic Periodontology"],"published-print":{"date-parts":[[2026,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Aim<\/jats:title>\n                    <jats:p>To evaluate the subgingival proteome and microbiome of mothers with periodontitis and their offspring, thereby assessing signatures of periodontal diseases.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Forty participants in four groups were included: mothers with periodontitis and their offspring, as well as periodontally healthy mothers and their offspring. Periodontal clinical parameters were assessed. Gingival crevicular fluid (GCF) and subgingival biofilm were collected from the same sites. Proteome from GCF was investigated by liquid chromatography\u2010tandem mass spectrometry with data\u2010independent acquisition (DIA\u2010PASEF). Bacterial DNA from subgingival biofilms was sequenced using the 16S rRNA gene for taxonomy assignment.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Overall, 6147 bacterial and human proteins (\u2265\u20092 peptides) were quantified. Despite the absence of attachment loss, the offspring of mothers with periodontitis presented with similar proteotypes as their mothers, characterised by up\u2010regulation of inflammatory response cascades and down\u2010regulation of epithelial barrier proteins. They also displayed higher colonisation patterns by periodontopathogens while presenting with increased expression of bacterial virulence proteins compared with controls.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>The study showed that the maternal periodontal microbiome and proteome associate with those of the offspring and relate to maternal periodontal status. These early ecological events may potentially promote offspring's susceptibility to dysbiosis and may predispose them to periodontitis.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/jcpe.70045","type":"journal-article","created":{"date-parts":[[2025,10,3]],"date-time":"2025-10-03T01:10:42Z","timestamp":1759453842000},"page":"201-213","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["The Subgingival Host\u2013Microbial Landscape in Mothers With Periodontitis and Their At\u2010Risk Offspring"],"prefix":"10.1111","volume":"53","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0619-2547","authenticated-orcid":false,"given":"H\u00e9lvis E. S.","family":"Paz","sequence":"first","affiliation":[{"name":"Division of Periodontics, Department of Prosthodontics and Periodontics, Faculdade de Odontologia de Piracicaba Universidade Estadual de Campinas  Piracicaba Brazil"},{"name":"Division of Oral Health and Periodontology, Department of Dental Medicine Karolinska Institutet  Stockholm Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Camila S.","family":"Stolf","sequence":"additional","affiliation":[{"name":"Division of Periodontics, Department of Prosthodontics and Periodontics, Faculdade de Odontologia de Piracicaba Universidade Estadual de Campinas  Piracicaba Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kai","family":"Bao","sequence":"additional","affiliation":[{"name":"Division of Oral Health and Periodontology, Department of Dental Medicine Karolinska Institutet  Stockholm Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9333-4349","authenticated-orcid":false,"given":"Mabelle F.","family":"Monteiro","sequence":"additional","affiliation":[{"name":"Division of Periodontics, Department of Prosthodontics and Periodontics, Faculdade de Odontologia de Piracicaba Universidade Estadual de Campinas  Piracicaba Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8766-0452","authenticated-orcid":false,"given":"Lucas M.","family":"Carvalho","sequence":"additional","affiliation":[{"name":"Post Graduate Program in Health Sciences S\u00e3o Francisco University  Bragan\u00e7a Paulista Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Angelika","family":"Silbereisen","sequence":"additional","affiliation":[{"name":"Division of Oral Health and Periodontology, Department of Dental Medicine Karolinska Institutet  Stockholm Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Georgios N.","family":"Belibasakis","sequence":"additional","affiliation":[{"name":"Division of Oral Health and Periodontology, Department of Dental Medicine Karolinska Institutet  Stockholm Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Renato C. V.","family":"Casarin","sequence":"additional","affiliation":[{"name":"Division of Periodontics, Department of Prosthodontics and Periodontics, Faculdade de Odontologia de Piracicaba Universidade Estadual de Campinas  Piracicaba Brazil"},{"name":"Department of Periodontics and Endodontics, School of Dental Medicine University at Buffalo  Buffalo New York USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6742-3556","authenticated-orcid":false,"given":"Nagihan","family":"Bostanci","sequence":"additional","affiliation":[{"name":"Division of Oral Health and Periodontology, Department of Dental Medicine Karolinska Institutet  Stockholm Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,10,2]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/ismej.2012.174"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/prd.12362"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/prd.12014"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep15999"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.3390\/pathogens11050590"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.201900253"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/prd.12473"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1080\/21505594.2015.1081731"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/jre.12061"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41587\u2010019\u20100209\u20109"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jproteome.8b00263"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/prd.12154"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/prd.12351"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1093\/database\/baq013"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2021.09.009"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/jcpe.12533"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/prd.12350"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.1111\/jre.12436"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00729\u201013"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.it.2021.08.001"},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.2353\/ajpath.2010.100168"},{"key":"e_1_2_11_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41579\u2010018\u20100089\u2010x"},{"key":"e_1_2_11_24_1","unstructured":"Leary P. andH.Rehrauer.2023.\u201cexploreDE Interactive Shiny App (2023.0.20). Zenodo.\u201dhttps:\/\/doi.org\/10.5281\/zenodo.8183763."},{"key":"e_1_2_11_25_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010020\u201017041\u20107"},{"key":"e_1_2_11_26_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12903\u2010021\u201001606\u2010z"},{"key":"e_1_2_11_27_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.1994.65.6.623"},{"key":"e_1_2_11_28_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41592\u2010020\u201000998\u20100"},{"key":"e_1_2_11_29_1","doi-asserted-by":"publisher","DOI":"10.1074\/mcp.TIR118.000900"},{"key":"e_1_2_11_30_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600\u20100757.2010.00367.x"},{"key":"e_1_2_11_31_1","doi-asserted-by":"publisher","DOI":"10.17796\/1053\u20104628\u201042.2.3"},{"key":"e_1_2_11_32_1","doi-asserted-by":"publisher","DOI":"10.1111\/jcpe.12459"},{"key":"e_1_2_11_33_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010020\u201080372\u20104"},{"key":"e_1_2_11_34_1","doi-asserted-by":"publisher","DOI":"10.1177\/0022034516634619"},{"key":"e_1_2_11_35_1","doi-asserted-by":"publisher","DOI":"10.1515\/jib\u20102022\u20100031"},{"key":"e_1_2_11_36_1","doi-asserted-by":"publisher","DOI":"10.1002\/JPER.17\u20100721"},{"key":"e_1_2_11_37_1","doi-asserted-by":"publisher","DOI":"10.1002\/JPER.21\u20100411"},{"key":"e_1_2_11_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140\u20106736(19)31146\u20108"},{"key":"e_1_2_11_39_1","doi-asserted-by":"publisher","DOI":"10.1111\/jcpe.13815"},{"key":"e_1_2_11_40_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471\u20102180\u201010\u201066"},{"key":"e_1_2_11_41_1","doi-asserted-by":"publisher","DOI":"10.1111\/jcpe.13990"},{"key":"e_1_2_11_42_1","doi-asserted-by":"publisher","DOI":"10.1002\/JPER.22\u20100356"},{"key":"e_1_2_11_43_1","doi-asserted-by":"publisher","DOI":"10.1002\/JPER.22\u20100317"},{"key":"e_1_2_11_44_1","doi-asserted-by":"publisher","DOI":"10.1111\/jre.13169"},{"key":"e_1_2_11_45_1","doi-asserted-by":"publisher","DOI":"10.1111\/prd.12021"},{"key":"e_1_2_11_46_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140\u20106736(07)61602\u2010X"},{"key":"e_1_2_11_47_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jproteome.3c00143"},{"key":"e_1_2_11_48_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13073\u2010015\u20100153\u20103"},{"key":"e_1_2_11_49_1","doi-asserted-by":"publisher","DOI":"10.1111\/jre.13408"}],"container-title":["Journal of Clinical Periodontology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jcpe.70045","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/jcpe.70045","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jcpe.70045","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,30]],"date-time":"2026-10-30T07:02:43Z","timestamp":1793343763000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/jcpe.70045"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,10,2]]},"references-count":48,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2026,2]]}},"alternative-id":["10.1111\/jcpe.70045"],"URL":"https:\/\/doi.org\/10.1111\/jcpe.70045","archive":["Portico"],"relation":{},"ISSN":["0303-6979","1600-051X"],"issn-type":[{"value":"0303-6979","type":"print"},{"value":"1600-051X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,10,2]]},"assertion":[{"value":"2025-06-01","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-09-24","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-10-02","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}