{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T17:56:24Z","timestamp":1777658184427,"version":"3.51.4"},"reference-count":41,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2025,10,7]],"date-time":"2025-10-07T00:00:00Z","timestamp":1759795200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,10,7]],"date-time":"2025-10-07T00:00:00Z","timestamp":1759795200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100000691","name":"Academy of Medical Sciences","doi-asserted-by":"publisher","award":["SGL024\\1051"],"award-info":[{"award-number":["SGL024\\1051"]}],"id":[{"id":"10.13039\/501100000691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000272","name":"National Institute for Health and Care Research","doi-asserted-by":"publisher","award":["CL\u20102019\u201017\u2010009"],"award-info":[{"award-number":["CL\u20102019\u201017\u2010009"]}],"id":[{"id":"10.13039\/501100000272","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Clinic Periodontology"],"published-print":{"date-parts":[[2026,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Aim<\/jats:title>\n                    <jats:p>To investigate the cellular composition and molecular mechanisms of periodontal granulation tissue formation using single\u2010cell RNA sequencing (scRNA\u2010seq), aiming to enhance the understanding of periodontal disease pathogenesis and identify potential targets for regenerative therapies.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Materials and Methods<\/jats:title>\n                    <jats:p>\n                      Granulation tissue samples were collected from patients undergoing periodontal surgery (\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u20093). Fresh tissues were processed into single\u2010cell suspensions and subjected to scRNA\u2010seq. The data were integrated and compared with existing datasets from healthy gingiva and periodontal ligament. Computational analyses were performed and validated through immunofluorescence staining.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Ten distinct cell clusters were identified across the samples. Granulation tissue exhibited a higher abundance of immune cells compared to healthy tissues. A novel endothelial cell subpopulation, exclusive to granulation tissue, was discovered and characterised by\n                      <jats:italic>NOTCH3<\/jats:italic>\n                      expression and involvement in ossification pathways. Additionally, granulation tissue fibroblast subpopulations demonstrated a progenitor\u2010like state, characterised by extracellular matrix reorganisation and low differentiation, similar to cancer\u2010associated fibroblasts.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>This study identified a novel endothelial subpopulation offering new insights into the disease's pathogenesis and presenting potential targets for regenerative therapies. These findings will help advance the understanding of periodontal disease granulation tissue formation and provide information for the development of materials to modulate specific cellular pathways to improve periodontal disease management.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/jcpe.70048","type":"journal-article","created":{"date-parts":[[2025,10,7]],"date-time":"2025-10-07T12:46:41Z","timestamp":1759841201000},"page":"308-320","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Characterising the Periodontal Granulation Tissue Using\n                    <scp>scRNAseq<\/scp>"],"prefix":"10.1111","volume":"53","author":[{"given":"Wentao","family":"Zhu","sequence":"first","affiliation":[{"name":"Department of Women's and Children's Health Karolinska Institutet  Stockholm Sweden"},{"name":"Science for Life Laboratory KTH \u2013 Royal Institute of Technology  Stockholm Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kathy","family":"Fung","sequence":"additional","affiliation":[{"name":"NIHR BRC Genomics Research Platform, Guy's and St Thomas' NHS Foundation Trust, King's College London School of Medicine  London UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pawan","family":"Dhami","sequence":"additional","affiliation":[{"name":"NIHR BRC Genomics Research Platform, Guy's and St Thomas' NHS Foundation Trust, King's College London School of Medicine  London UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paul","family":"Sharpe","sequence":"additional","affiliation":[{"name":"Centre for Craniofacial and Regenerative Biology, FoDOCS, King's College London  London UK"},{"name":"Institute of Animal Physiology and Genetics  Brno Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jan","family":"Krivanek","sequence":"additional","affiliation":[{"name":"Department of Histology and Embryology, Faculty of Medicine Masaryk University  Brno Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7750-5010","authenticated-orcid":false,"given":"Luigi","family":"Nibali","sequence":"additional","affiliation":[{"name":"Periodontology Unit, Centre for Host\u2010Microbiome Interactions, FoDOCS, King's College London  London UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cheng","family":"Zhang","sequence":"additional","affiliation":[{"name":"Science for Life Laboratory KTH \u2013 Royal Institute of Technology  Stockholm Sweden"},{"name":"Faculty of Life Sciences &amp; Medicine, School of Immunology &amp; Microbial Sciences, James Black Centre, King's College London The Roger Williams Institute of Liver Studies  London UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5111-269X","authenticated-orcid":false,"given":"Vitor C. 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