{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T07:06:00Z","timestamp":1791270360014,"version":"4.1.0"},"reference-count":50,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2025,11,24]],"date-time":"2025-11-24T00:00:00Z","timestamp":1763942400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,11,24]],"date-time":"2025-11-24T00:00:00Z","timestamp":1763942400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82370971"],"award-info":[{"award-number":["82370971"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Clinic Periodontology"],"published-print":{"date-parts":[[2026,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Aim<\/jats:title>\n                    <jats:p>To investigate the impact of neutrophil extracellular traps (NETs) on periodontitis and the regulatory mechanisms by which melatonin alleviates the disease.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Materials and Methods<\/jats:title>\n                    <jats:p>The expression of NET markers in periodontitis tissues was detected using multiple immunohistochemical staining. The effect of NETs on periodontitis was explored through in\u00a0vivo and in\u00a0vitro experiments. qPCR\u00a0and\u00a0transwell migration assay were used to assess neutrophil activation and recruitment. Flow cytometry, micro\u2010CT\u00a0and histological\u00a0staining were employed to evaluate neutrophil recruitment in gingiva and periodontitis progression\u00a0in\u00a0a\u00a0ligature\u2010induced periodontitis (LIP) mouse model. Melatonin was then administered in\u00a0vivo and in\u00a0vitro to evaluate\u00a0its\u00a0effects on\u00a0NET\u00a0formation, neutrophil activation, neutrophil recruitment and periodontitis progression. Finally, Western blotting and qPCR were employed to elucidate the mechanisms underlying melatonin\u2010mediated regulation of NET formation.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>NETs were evident in gingival tissues from patients with periodontitis. After inhibiting NET formation, the expression levels of inflammatory cytokines (Il\u20101\u03b2, Il\u20106, Tnf\u2010\u03b1), neutrophil chemokines (Ccl2, Ccl4, Cxcl1) and neutrophil recruitment were found to decrease. Furthermore, melatonin treatment reduced NET formation, decreased neutrophil activation and recruitment and alleviated bone resorption in LIP mice. Mechanistically, melatonin suppresses NET formation through the NF\u2010\u03baB signalling pathway.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Melatonin decreases neutrophil recruitment by suppressing NET formation, exerting a protective effect in periodontitis.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/jcpe.70067","type":"journal-article","created":{"date-parts":[[2025,11,24]],"date-time":"2025-11-24T23:10:47Z","timestamp":1764025847000},"page":"630-643","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Melatonin Alleviates Periodontitis by Suppressing Neutrophil Extracellular Traps\u2013Dependent Neutrophil Recruitment"],"prefix":"10.1111","volume":"53","author":[{"ORCID":"https:\/\/orcid.org\/0009-0004-4292-0259","authenticated-orcid":false,"given":"Xiao","family":"Wu","sequence":"first","affiliation":[{"name":"Shanghai Engineering Research Center of Tooth Restoration and Regeneration &amp; Tongji Research Institute of Stomatology &amp; Department of Periodontics Stomatological Hospital and Dental School, Tongji University  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7304-7067","authenticated-orcid":false,"given":"Jiawei","family":"Lu","sequence":"additional","affiliation":[{"name":"Shanghai Engineering Research Center of Tooth Restoration and Regeneration &amp; Tongji Research Institute of Stomatology &amp; Department of Periodontics Stomatological Hospital and Dental School, Tongji University  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zehui","family":"Xiong","sequence":"additional","affiliation":[{"name":"Shanghai Engineering Research Center of Tooth Restoration and Regeneration &amp; Tongji Research Institute of Stomatology &amp; Department of Periodontics Stomatological Hospital and Dental School, Tongji University  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jie","family":"Huang","sequence":"additional","affiliation":[{"name":"Shanghai Engineering Research Center of Tooth Restoration and Regeneration &amp; Tongji Research Institute of Stomatology &amp; Department of Periodontics Stomatological Hospital and Dental School, Tongji University  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinyi","family":"Zhang","sequence":"additional","affiliation":[{"name":"Shanghai Engineering Research Center of Tooth Restoration and Regeneration &amp; Tongji Research Institute of Stomatology &amp; Department of Periodontics Stomatological Hospital and Dental School, Tongji University  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7137-1739","authenticated-orcid":false,"given":"Lijun","family":"Luo","sequence":"additional","affiliation":[{"name":"Shanghai Engineering Research Center of Tooth Restoration and Regeneration &amp; Tongji Research Institute of Stomatology &amp; Department of Periodontics Stomatological Hospital and Dental School, Tongji University  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,11,24]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1155\/2021\/8840167"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.2337\/db24\u20100481"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/jpi.12667"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/jre.12010"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1136\/gutjnl\u20102023\u2010331447"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1177\/0022034521995157"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.3390\/cells11121919"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/prd.12611"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.apsb.2023.01.020"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/jcpe.14025"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.2006.050287"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10266\u2010013\u20100122\u20105"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.4317\/medoral.18832"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/nri.2016.49"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2019.02536"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pharmthera.2023.108453"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/jcpe.13952"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41392\u2010024\u201001757\u20109"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/prd.12313"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.redox.2023.102787"},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.1111\/jpi.12996"},{"key":"e_1_2_11_23_1","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00062.2021"},{"key":"e_1_2_11_24_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2021.627328"},{"key":"e_1_2_11_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0891-5849(00)00368-3"},{"key":"e_1_2_11_26_1","doi-asserted-by":"publisher","DOI":"10.1038\/s12276\u2010024\u201001219\u2010w"},{"key":"e_1_2_11_27_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20221751"},{"key":"e_1_2_11_28_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.114.303312"},{"key":"e_1_2_11_29_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2424169122"},{"key":"e_1_2_11_30_1","doi-asserted-by":"publisher","DOI":"10.1111\/jpi.12414"},{"key":"e_1_2_11_31_1","doi-asserted-by":"publisher","DOI":"10.1002\/med.21628"},{"key":"e_1_2_11_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140\u20106736(22)00877\u20107"},{"key":"e_1_2_11_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.it.2017.08.005"},{"key":"e_1_2_11_34_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41581\u2010025\u201000944\u20103"},{"key":"e_1_2_11_35_1","doi-asserted-by":"publisher","DOI":"10.1111\/jpi.12676"},{"key":"e_1_2_11_36_1","doi-asserted-by":"publisher","DOI":"10.1111\/jcpe.12946"},{"key":"e_1_2_11_37_1","doi-asserted-by":"publisher","DOI":"10.1038\/nri.2017.105"},{"key":"e_1_2_11_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jare.2024.07.028"},{"key":"e_1_2_11_39_1","doi-asserted-by":"publisher","DOI":"10.1111\/jpi.12732"},{"key":"e_1_2_11_40_1","doi-asserted-by":"publisher","DOI":"10.1111\/prd.12342"},{"key":"e_1_2_11_41_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41419\u2010021\u201003896\u20101"},{"key":"e_1_2_11_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.canlet.2018.11.037"},{"key":"e_1_2_11_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.heliyon.2024.e32494"},{"key":"e_1_2_11_44_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.aaa8064"},{"key":"e_1_2_11_45_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41577\u2010022\u201000787\u20100"},{"key":"e_1_2_11_46_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm.3887"},{"key":"e_1_2_11_47_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ecoenv.2023.115923"},{"key":"e_1_2_11_48_1","doi-asserted-by":"publisher","DOI":"10.1111\/jpi.70039"},{"key":"e_1_2_11_49_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41392\u2010020\u201000312\u20106"},{"key":"e_1_2_11_50_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.arr.2022.101635"},{"key":"e_1_2_11_51_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pharmthera.2022.108328"}],"container-title":["Journal of Clinical Periodontology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jcpe.70067","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/jcpe.70067","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jcpe.70067","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,3,10]],"date-time":"2026-03-10T06:14:30Z","timestamp":1773123270000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/jcpe.70067"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,11,24]]},"references-count":50,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2026,4]]}},"alternative-id":["10.1111\/jcpe.70067"],"URL":"https:\/\/doi.org\/10.1111\/jcpe.70067","archive":["Portico"],"relation":{},"ISSN":["0303-6979","1600-051X"],"issn-type":[{"value":"0303-6979","type":"print"},{"value":"1600-051X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,11,24]]},"assertion":[{"value":"2025-06-18","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-10-22","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-11-24","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}