{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T02:57:36Z","timestamp":1777431456208,"version":"3.51.4"},"reference-count":39,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2026,1,14]],"date-time":"2026-01-14T00:00:00Z","timestamp":1768348800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,1,14]],"date-time":"2026-01-14T00:00:00Z","timestamp":1768348800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Clinic Periodontology"],"published-print":{"date-parts":[[2026,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Aims<\/jats:title>\n                    <jats:p>To investigate the associations between oral\u2010rinse microbiota and distinct oral conditions, and further evaluate its potential ability to distinguish periodontitis severity.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Oral\u2010rinse\u2010sourced microbiota with 16S ribosomal RNA sequencing from 3770 adults in US National Health and Nutrition Examination Survey 2009\u20132012 were analysed across oral health, caries, periodontitis, co\u2010existing caries and periodontitis and edentulism. Diagnostic potential of the oral\u2010rinse microbiota for periodontitis severity was evaluated using multi\u2010class random forest (RF) model with internal validation and external validation in an independent cohort (\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u2009392).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Oral condition accounted for substantial variance in oral\u2010rinse microbiota, revealing disease or tooth loss\u2013associated shifts. Increasing acidogenic\/aciduric taxa (\n                      <jats:italic>Veillonella<\/jats:italic>\n                      ,\n                      <jats:italic>Lactobacillus<\/jats:italic>\n                      ,\n                      <jats:italic>Atopobium<\/jats:italic>\n                      ) or periodontitis\u2010associated taxa (\n                      <jats:italic>Filifactor<\/jats:italic>\n                      ,\n                      <jats:italic>Treponema<\/jats:italic>\n                      ,\n                      <jats:italic>Tannerella<\/jats:italic>\n                      ) were identified in caries\u2010only or periodontitis\u2010only groups, respectively, while the co\u2010existing disease group showed overlapping shifts. Taxa shifted dose\u2010dependently with increasing periodontitis severity. The RF model achieved moderate performance in identifying severe periodontitis, with the area under the receiver operating characteristic curve (AUROC) of 0.81 (0.75\u20130.87) internally and 0.83 (0.77\u20130.88) externally. Key contributing taxa aligned with established periodontitis\u2010associated genera, supporting model interpretability.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Based on our results, oral\u2010rinse microbiota captures disease\u2010specific signatures across oral conditions, supporting its potential as a non\u2010invasive tool to monitor oral microbial ecology and assess periodontitis severity at the population level.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/jcpe.70101","type":"journal-article","created":{"date-parts":[[2026,1,15]],"date-time":"2026-01-15T00:40:29Z","timestamp":1768437629000},"page":"760-773","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Oral\u2010Rinse\u2010Sourced Microbiota in Oral Health and Diseases in a Representative US Adult 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