{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T02:06:35Z","timestamp":1790215595869,"version":"4.1.0"},"reference-count":39,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2026,2,9]],"date-time":"2026-02-09T00:00:00Z","timestamp":1770595200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,2,9]],"date-time":"2026-02-09T00:00:00Z","timestamp":1770595200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100032645","name":"Hevolution Foundation","doi-asserted-by":"publisher","award":["HF\u2010GRO\u201023\u20101199172\u201046"],"award-info":[{"award-number":["HF\u2010GRO\u201023\u20101199172\u201046"]}],"id":[{"id":"10.13039\/100032645","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000049","name":"National Institute on Aging","doi-asserted-by":"publisher","award":["R01AG064003"],"award-info":[{"award-number":["R01AG064003"]}],"id":[{"id":"10.13039\/100000049","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000049","name":"National Institute on Aging","doi-asserted-by":"publisher","award":["NIAR25AG060892"],"award-info":[{"award-number":["NIAR25AG060892"]}],"id":[{"id":"10.13039\/100000049","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000738","name":"U.S. Department of Veterans Affairs","doi-asserted-by":"publisher","award":["I21BX006307"],"award-info":[{"award-number":["I21BX006307"]}],"id":[{"id":"10.13039\/100000738","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Clinic Periodontology"],"published-print":{"date-parts":[[2026,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Aim(s)<\/jats:title>\n                    <jats:p>This cross\u2010sectional clinical study aimed to explore the untargeted metabolomic and lipidomic profiles in gingival crevicular fluid (GCF) obtained from healthy individuals and patients with periodontitis.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Materials and Methods<\/jats:title>\n                    <jats:p>GCF was collected from 17 periodontally healthy and 19 periodontitis patients. For the unbiased characterisation of positively and negatively charged polar metabolite and lipid compounds, we employed hydrophilic interaction and reversed\u2010phase liquid chromatography\/mass spectrometry platforms, followed by a semisupervised deep learning\u2010based approach for metabolomic peak curation and data analysis.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      A total of 256 metabolites were identified with Metabolomics Standards Initiative (MSI) confidence Levels 1\u20133, including 198 elevated and 58 diminished compounds in periodontal lesions (\n                      <jats:italic>q<\/jats:italic>\n                      \u2009&lt;\u20090.05, |log\n                      <jats:sub>2<\/jats:sub>\n                      \u2010transformed Fold Change (FC)|\u2009&gt;\u20091). Periodontitis samples exhibited a significant positive accumulation of purine degradation and ceramide metabolites, along with a negative regulation of oxy fatty acids metabolism. Additionally, we observed a significant increase in established periodontitis biomarkers, including N\u2010acetylneuraminic acid, citrulline and 2\u2010pyrrolidineacetic acid. The study also characterised distinct differences in bacterial and fungal metabolite profiles between the healthy and diseased samples.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>These findings suggest that untargeted metabolomic screening of GCF may significantly improve our understanding of biochemical changes between healthy tissue and periodontitis. This knowledge is pivotal for the development of a precision paradigm in periodontitis.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/jcpe.70105","type":"journal-article","created":{"date-parts":[[2026,2,10]],"date-time":"2026-02-10T07:27:35Z","timestamp":1770708455000},"page":"774-783","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Untargeted Metabolomic and Lipidomic Profiles of Gingival Crevicular Fluid in the Context of Periodontitis"],"prefix":"10.1111","volume":"53","author":[{"given":"Caroline H.","family":"Henderzahs","sequence":"first","affiliation":[{"name":"Department of Periodontology Indiana University School of Dentistry  Indianapolis Indiana USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chiaki","family":"Yamada","sequence":"additional","affiliation":[{"name":"Department of Oral Biology and Comprehensive Science Indiana University School of Dentistry  Indianapolis Indiana USA"},{"name":"Indiana Center for Musculoskeletal Health Indiana University School of Medicine  Indianapolis Indiana USA"},{"name":"Richard L. Roudebush VA Medical Center  Indianapolis Indiana USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexandr","family":"Morozov","sequence":"additional","affiliation":[{"name":"Department of Oral Biology and Comprehensive Science Indiana University School of Dentistry  Indianapolis Indiana USA"},{"name":"Indiana Center for Musculoskeletal Health Indiana University School of Medicine  Indianapolis Indiana USA"},{"name":"Institute of Zoology, State University of Moldova  Chisinau Republic of Moldova"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thelmalane","family":"Yalartai","sequence":"additional","affiliation":[{"name":"Department of Oral Biology and Comprehensive Science Indiana University School of Dentistry  Indianapolis Indiana USA"},{"name":"Indiana Center for Musculoskeletal Health Indiana University School of Medicine  Indianapolis Indiana USA"},{"name":"Department of Biology St. Catherine University  St. Paul Minnesota USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vanchit","family":"John","sequence":"additional","affiliation":[{"name":"Department of Periodontology Indiana University School of Dentistry  Indianapolis Indiana USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6489-4643","authenticated-orcid":false,"given":"Hawra","family":"AlQallaf","sequence":"additional","affiliation":[{"name":"Department of Periodontology Indiana University School of Dentistry  Indianapolis Indiana USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9827-7347","authenticated-orcid":false,"given":"Alexandru","family":"Movila","sequence":"additional","affiliation":[{"name":"Department of Oral Biology and Comprehensive Science Indiana University School of Dentistry  Indianapolis Indiana USA"},{"name":"Indiana Center for Musculoskeletal Health Indiana University School of Medicine  Indianapolis Indiana USA"},{"name":"Richard L. 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