{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,17]],"date-time":"2026-04-17T01:53:57Z","timestamp":1776390837148,"version":"3.51.2"},"reference-count":62,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2026,2,24]],"date-time":"2026-02-24T00:00:00Z","timestamp":1771891200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,2,24]],"date-time":"2026-02-24T00:00:00Z","timestamp":1771891200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100003803","name":"University of Hong Kong","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003803","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Clinic Periodontology"],"published-print":{"date-parts":[[2026,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Aim<\/jats:title>\n                    <jats:p>To investigate the intra\u2010tissue bacteriome and cell profiles within periodontal granulation tissue (PGT) for exploring the biological essence and translational potentials.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Materials and Methods<\/jats:title>\n                    <jats:p>PGT samples were collected from 49 patients with severe periodontitis\u2014including those from osseous defects (GT) during periodontal surgery, extraction sockets (ST) and extracted root surfaces (RT) during tooth extraction\u2014while the excised pocket wall (PT) from surgical sites served as the control for diseased tissues. These samples underwent 16S rRNA sequencing, single\u2010cell sequencing and histological assessment.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      GT and PT exhibited periodontal health\u2013associated, commensal\u2010enriched bacteriome profiles, while RT and ST showed worse local periodontal condition, enriched periodontopathogens but depleted commensals. Notably, GT contained a higher proportion of mesenchymal stem cells (MSCs) and fibroblasts while fewer natural killer cells than in PT and ST. Pseudotime trajectory analysis revealed endothelial and epithelial differentiation fates from mesenchymal progenitors across tissues. Moreover, there were less inflammatory infiltration and immunoreactivity of CD4\n                      <jats:sup>+<\/jats:sup>\n                      and NKG2D\n                      <jats:sup>+<\/jats:sup>\n                      in GT than in PT and ST.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Our findings suggest potential periodontal health\u2013associated features of GT regarding clinical, bacteriome and cellular attributes. Future translational study is warranted to explore GT as an alternative source of MSCs for periodontal regeneration.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/jcpe.70108","type":"journal-article","created":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T17:59:53Z","timestamp":1772992793000},"page":"806-820","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Intra\u2010Tissue Bacteriome and Cellular Profiles in Periodontal Granulation Tissue From Osseous Defects and\u00a0Extraction Sockets"],"prefix":"10.1111","volume":"53","author":[{"given":"Tianfan","family":"Cheng","sequence":"first","affiliation":[{"name":"Division of Periodontology &amp; Implant Dentistry, Faculty of Dentistry The University of Hong Kong  Hong Kong SAR China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3570-5564","authenticated-orcid":false,"given":"Tianle","family":"Li","sequence":"additional","affiliation":[{"name":"Division of Restorative Dental Sciences, Faculty of Dentistry The University of Hong Kong  Hong Kong SAR China"},{"name":"Division of Applied Oral Sciences &amp; Community Dental Care, Faculty of Dentistry The University of Hong Kong  Hong Kong SAR China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tsz Yung","family":"Wong","sequence":"additional","affiliation":[{"name":"Division of Periodontology &amp; Implant Dentistry, Faculty of Dentistry The University of Hong Kong  Hong Kong SAR China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Beibei","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Orthodontics Stomatological Hospital and Dental School of Tongji University  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7494-5515","authenticated-orcid":false,"given":"Chongshan","family":"Liao","sequence":"additional","affiliation":[{"name":"Department of Orthodontics Stomatological Hospital and Dental School of Tongji University  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xun","family":"Ding","sequence":"additional","affiliation":[{"name":"Division of Periodontology &amp; Implant Dentistry, Faculty of Dentistry The University of Hong Kong  Hong Kong SAR China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hui","family":"Chen","sequence":"additional","affiliation":[{"name":"Division of Restorative Dental Sciences, Faculty of Dentistry The University of Hong Kong  Hong Kong SAR China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0450-0366","authenticated-orcid":false,"given":"Wei","family":"Qiao","sequence":"additional","affiliation":[{"name":"Division of Applied Oral Sciences &amp; Community Dental Care, Faculty of Dentistry The University of Hong Kong  Hong Kong SAR China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1917-1988","authenticated-orcid":false,"given":"George","family":"Pelekos","sequence":"additional","affiliation":[{"name":"Division of Periodontology &amp; Implant Dentistry, Faculty of Dentistry The University of Hong Kong  Hong Kong SAR China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5340-9710","authenticated-orcid":false,"given":"Lijian","family":"Jin","sequence":"additional","affiliation":[{"name":"Division of Periodontology &amp; Implant Dentistry, Faculty of Dentistry The University of Hong Kong  Hong Kong SAR China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2026,2,24]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/cre2.532"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.3238\/dzz\u2010int.2020.0082\u20100094"},{"issue":"6","key":"e_1_2_10_4_1","first-page":"1133","article-title":"Bone Tissue Formation in Extraction Sockets From Sites With Advanced Periodontal Disease: A Histomorphometric Study in Humans","volume":"23","author":"Ahn J. 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