{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,13]],"date-time":"2026-05-13T03:16:44Z","timestamp":1778642204339,"version":"3.51.4"},"reference-count":37,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2026,3,26]],"date-time":"2026-03-26T00:00:00Z","timestamp":1774483200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"},{"start":{"date-parts":[[2026,3,26]],"date-time":"2026-03-26T00:00:00Z","timestamp":1774483200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["20K23020"],"award-info":[{"award-number":["20K23020"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["21K16984"],"award-info":[{"award-number":["21K16984"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["23K16017"],"award-info":[{"award-number":["23K16017"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Clinic Periodontology"],"published-print":{"date-parts":[[2026,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Aim<\/jats:title>\n                    <jats:p>To clarify the molecular mechanisms of occlusal trauma in bone loss through periodontal tissue transcriptome analysis in mice with periodontitis and traumatic occlusion.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Materials and Methods<\/jats:title>\n                    <jats:p>Ligature\u2010induced periodontitis (Li) and composite resin\u2010induced traumatic occlusion (Tra) mouse models were established (control [Co], Li, Tra and LiTra). Bone resorption was evaluated using micro\u2010computed tomography (micro\u2010CT). RNA\u2010sequencing was conducted on gingiva, bone and periodontal ligament from all groups 3\u2009days post induction. For long\u2010term evaluation, the Co and Tra groups were maintained for 8\u2009weeks and then analysed using micro\u2010CT and qRT\u2010PCR.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Traumatic occlusion alone, sustained for 8\u2009weeks, did not directly induce bone resorption; however, it significantly exacerbated bone resorption in mice with periodontitis. Cytokine\u2013cytokine receptor interactions and Toll\u2010like receptor signalling pathways were up\u2010regulated in LiTra bone tissue.\n                      <jats:italic>Il11, Il1rl1<\/jats:italic>\n                      and\n                      <jats:italic>Mmp3<\/jats:italic>\n                      , associated with inflammation and bone metabolism, were more highly expressed in the LiTra group than in the Li group. TNF\u2010\u03b1 signalling via NF\u03baB and inflammatory response gene sets were enriched in the bone tissue of LiTra group.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Traumatic occlusion accelerates bone resorption in ligature\u2010induced periodontitis but does not independently cause significant bone loss. Occlusal trauma enhances the expression of inflammation\u2010related genes, especially in bone with periodontitis.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/jcpe.70112","type":"journal-article","created":{"date-parts":[[2026,3,26]],"date-time":"2026-03-26T07:43:49Z","timestamp":1774511029000},"page":"838-848","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Traumatic Occlusion Exacerbates Bone Resorption by Modifying Gene Expression in the Bone Tissue of Ligature\u2010Induced Periodontitis in Mice"],"prefix":"10.1111","volume":"53","author":[{"ORCID":"https:\/\/orcid.org\/0009-0006-8364-2130","authenticated-orcid":false,"given":"Yosuke","family":"Tsuchiya","sequence":"first","affiliation":[{"name":"Department of Periodontology Graduate School of Medical and Dental Sciences, Institute of Science Tokyo  Tokyo 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