{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T00:34:54Z","timestamp":1791938094234,"version":"4.3.4"},"reference-count":60,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2026,3,26]],"date-time":"2026-03-26T00:00:00Z","timestamp":1774483200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,3,26]],"date-time":"2026-03-26T00:00:00Z","timestamp":1774483200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100004799","name":"Australian Dental Research Foundation","doi-asserted-by":"publisher","award":["534\u20102019"],"award-info":[{"award-number":["534\u20102019"]}],"id":[{"id":"10.13039\/501100004799","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000925","name":"National Health and Medical Research Council","doi-asserted-by":"publisher","award":["2034591"],"award-info":[{"award-number":["2034591"]}],"id":[{"id":"10.13039\/501100000925","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82001421"],"award-info":[{"award-number":["82001421"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Clinic Periodontology"],"published-print":{"date-parts":[[2026,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Aim<\/jats:title>\n                    <jats:p>Salivary microvesicles (MVs) are nanosized extracellular vesicles from the host and microbiota whose cargo may mirror the biological state of their parent cells. This cross\u2010sectional study aimed to explore the diagnostic power of host methylome and microbiome profiles of MVs in various periodontal disease states.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Materials and Methods<\/jats:title>\n                    <jats:p>This exploratory study recruited 20 healthy, 16 gingivitis and 26 stage III\/IV periodontitis cases. The origins of salivary host\u2010MVs were identified using a multiplex extracellular vesicle (EV) kit. The microbiome and host methylome profiles of MV DNA were analysed using 16S rRNA sequencing and methylated DNA immunoprecipitation sequencing (MeDIP\u2010seq), respectively.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      The periodontitis group showed increased CD63+, CD45+, CD29\n                      <jats:sup>+<\/jats:sup>\n                      and CD24\n                      <jats:sup>+<\/jats:sup>\n                      MV subpopulations (AUC\u2009&gt;\u20090.7), along with significantly higher bacterial outer membrane vesicles (AUC\u2009&gt;\u20090.89) from\n                      <jats:italic>Treponema<\/jats:italic>\n                      ,\n                      <jats:italic>Fretibacterium<\/jats:italic>\n                      and\n                      <jats:styled-content style=\"fixed-case\">\n                        <jats:italic>Treponema denticola<\/jats:italic>\n                      <\/jats:styled-content>\n                      , compared to both healthy and gingivitis groups, as well as the non\u2010periodontitis (combining healthy and gingivitis) group. MeDIP\u2010seq identified 1196 differentially methylated regions across 3\u2032 UTRs, CDS, introns and intergenic regions (AUC\u2009&gt;\u20090.9), distinguishing periodontitis from the other groups. These methylated genes were enriched in inflammation\u2010related pathways, including AMP\u2010activated protein kinase (AMPK) and Toll\u2010like receptor 4 (TLR4) pathways.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>This exploratory study found that host methylome and microbiome profiles in salivary MVs reflect periodontal disease status, and hence supports their potential as non\u2010invasive liquid biopsy biomarkers for periodontitis.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/jcpe.70114","type":"journal-article","created":{"date-parts":[[2026,3,27]],"date-time":"2026-03-27T02:41:36Z","timestamp":1774579296000},"page":"911-923","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Salivary Microvesicle Methylome and Microbiome Profiles in Periodontitis: An Exploratory Study"],"prefix":"10.1111","volume":"53","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7546-1635","authenticated-orcid":false,"given":"Pingping","family":"Han","sequence":"first","affiliation":[{"name":"School of Dentistry, The University of Queensland  Brisbane Queensland Australia"},{"name":"Center for Orofacial Regeneration, Rehabilitation and Reconstruction (COR3), Epigenetics Nanodiagnostic and Therapeutic Group, The University of Queensland  Brisbane Queensland Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chaminda","family":"Seneviratne","sequence":"additional","affiliation":[{"name":"School of Dentistry, The University of Queensland  Brisbane Queensland Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiongyi","family":"Zhao","sequence":"additional","affiliation":[{"name":"The University of Queensland, Queensland Brain Institute  Brisbane Queensland 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China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5339-0936","authenticated-orcid":false,"given":"Sa\u0161o","family":"Ivanovski","sequence":"additional","affiliation":[{"name":"School of Dentistry, The University of Queensland  Brisbane Queensland Australia"},{"name":"Center for Orofacial Regeneration, Rehabilitation and Reconstruction (COR3), Epigenetics Nanodiagnostic and Therapeutic Group, The University of Queensland  Brisbane Queensland 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