{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T05:59:06Z","timestamp":1790315946583,"version":"4.1.0"},"reference-count":35,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,8,23]],"date-time":"2026-08-23T00:00:00Z","timestamp":1787443200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2026,8,23]],"date-time":"2026-08-23T00:00:00Z","timestamp":1787443200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Clinic Periodontology"],"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Aim<\/jats:title>\n                    <jats:p>Risk assessment tools for periodontitis lack sufficient precision to predict disease progression. The aim of this exploratory study was to evaluate proteolytic activity (PA) in gingival crevicular fluid in relation to clinically assessed progression risk, microbial community composition and host proteome.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Materials and Methods<\/jats:title>\n                    <jats:p>Samples were collected from 109 patients with periodontitis and stratified into high and low PA groups using a fluorescent substrate. Microbial composition was analysed via 16S rRNA gene sequencing, and host proteomic profiles were determined using mass spectrometry.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Forty\u2010one of 42 patients in the high PA group were clinically classified as high\u2010risk, while the low\u2010activity group included both low\u2010 and high\u2010risk individuals. High\u2010activity samples displayed greater microbial diversity and enrichment of proteolytic species including\n                      <jats:italic>\n                        <jats:styled-content style=\"fixed-case\">Fusobacterium nucleatum<\/jats:styled-content>\n                        ,\n                        <jats:styled-content style=\"fixed-case\">Porphyromonas gingivalis<\/jats:styled-content>\n                      <\/jats:italic>\n                      and\n                      <jats:styled-content style=\"fixed-case\">\n                        <jats:italic>Treponema denticola<\/jats:italic>\n                      <\/jats:styled-content>\n                      . Of the 235 differentially expressed host proteins, high\u2010activity samples had elevated markers of inflammation and neutrophil\u2010mediated immunity, whereas low\u2010activity samples were enriched in protease inhibitors, indicating more effective regulation of tissue degradation.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Small\u2010volume gingival crevicular fluid sampling allows integrated analysis of microbial and host signatures that provide insight into the pathobiology of periodontitis. PA stratifies patients according to these microbial and host proteome signatures.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/jcpe.70186","type":"journal-article","created":{"date-parts":[[2026,8,24]],"date-time":"2026-08-24T02:27:26Z","timestamp":1787538446000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Distinct Microbial and Host Proteome Signatures Relate to Proteolytic Activity in Gingival Crevicular Fluid in an Exploratory Study of Patients With Periodontitis"],"prefix":"10.1111","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9566-0321","authenticated-orcid":false,"given":"Miguel","family":"Carda\u2010Di\u00e9guez","sequence":"first","affiliation":[{"name":"Department of Health and Genomics Foundation for the Promotion of Health and Biomedical Research (FISABIO) Foundation  Valencia Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stefan","family":"R\u00fcdiger","sequence":"additional","affiliation":[{"name":"Department of Periodontology Specialist Dental Care Centre, Public Dental Service  Malm\u00f6 Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3173-7577","authenticated-orcid":false,"given":"Gunnel","family":"Svens\u00e4ter","sequence":"additional","affiliation":[{"name":"Section for Oral Biology and Pathology, Faculty of Odontology Malm\u00f6 University  Malm\u00f6 Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Henrik","family":"Jansson","sequence":"additional","affiliation":[{"name":"Folktandv\u00e5rden Sk\u00e5ne The Swedish Dental Service of Sk\u00e5ne  Lund Sweden"},{"name":"The Institute for Postgraduate Dental Education, Region J\u00f6nk\u00f6ping County  J\u00f6nk\u00f6ping Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tirthankar","family":"Mohanty","sequence":"additional","affiliation":[{"name":"Division of Infection Medicine, Department of Clinical Sciences, Lund, Faculty of Medicine Lund University  Lund Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0295-3142","authenticated-orcid":false,"given":"Yashuan","family":"Chao","sequence":"additional","affiliation":[{"name":"Section for Oral Biology and Pathology, Faculty of Odontology Malm\u00f6 University  Malm\u00f6 Sweden"},{"name":"Division of Infection Medicine, Department of Clinical Sciences, Lund, Faculty of Medicine Lund University  Lund Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7099-0092","authenticated-orcid":false,"given":"Christofer","family":"Karlsson","sequence":"additional","affiliation":[{"name":"Division of Infection Medicine, Department of Clinical Sciences, Lund, Faculty of Medicine Lund University  Lund Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2889-7169","authenticated-orcid":false,"given":"Johan","family":"Malmstr\u00f6m","sequence":"additional","affiliation":[{"name":"Division of Infection Medicine, Department of Clinical Sciences, Lund, Faculty of Medicine Lund University  Lund Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Oonagh","family":"Shannon","sequence":"additional","affiliation":[{"name":"Section for Oral Biology and Pathology, Faculty of Odontology Malm\u00f6 University  Malm\u00f6 Sweden"},{"name":"Biofilms Research Center for Biointerfaces Malm\u00f6 University  Malm\u00f6 Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9127-3877","authenticated-orcid":false,"given":"\u00c1lex","family":"Mira","sequence":"additional","affiliation":[{"name":"Department of Health and Genomics Foundation for the Promotion of Health and Biomedical Research (FISABIO) Foundation  Valencia Spain"},{"name":"School of Health &amp; 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