{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T03:56:57Z","timestamp":1790654217063,"version":"4.1.0"},"reference-count":38,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T00:00:00Z","timestamp":1790553600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T00:00:00Z","timestamp":1790553600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82301071"],"award-info":[{"award-number":["82301071"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Clinic Periodontology"],"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Aim<\/jats:title>\n                    <jats:p>To examine the association between metal profiles in plasma and periodontitis and explore zinc's role in inflammatory pathways using multi\u2010omics and cellular approaches.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Materials and Methods<\/jats:title>\n                    <jats:p>A case\u2013control study including 424 individuals was conducted. Plasma levels of 35 metals were measured by inductively coupled plasma mass spectrometry. Logistic regression, Bayesian kernel machine regression, weighted quantile sum regression and quantile g\u2010computation were used to assess single\u2010 and mixed\u2010metal associations. Transcriptomics, network pharmacology and virtual knockout analyses explored inflammatory pathways related to reduced zinc. Cellular experiments using TPEN in hPDLFs provided exploratory biological context.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Zinc showed the strongest inverse association with periodontitis (OR\u2009=\u20090.299, 95% CI: 0.185\u20130.446). Multi\u2010metal models consistently identified zinc as the primary inversely associated element (WQS weight\u2009=\u20090.690; g\u2010computation weight\u2009=\u2009\u22120.622). Multi\u2010omics analyses indicated enrichment of the NF\u2010\u03baB\/NLRP3 inflammasome pathway. Zinc chelation was associated with increased expression of\n                      <jats:italic>IL1B<\/jats:italic>\n                      ,\n                      <jats:italic>IL18<\/jats:italic>\n                      ,\n                      <jats:italic>NLRP3<\/jats:italic>\n                      ,\n                      <jats:italic>CASP1<\/jats:italic>\n                      and\n                      <jats:italic>RELA<\/jats:italic>\n                      in\u00a0vitro. Virtual knockout analyses suggested the involvement of cytoskeleton regulation, integrin signalling and fibroblast transition.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>This exploratory study supports an inverse association between plasma zinc and periodontitis in a multi\u2010metal context. Multi\u2010omics and cellular results are hypothesis\u2010generating only, and causal or preventive implications require confirmation from prospective and experimental studies.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/jcpe.70207","type":"journal-article","created":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T03:21:02Z","timestamp":1790652062000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Zinc Inversely Associates With Periodontitis Risk: An Exploratory Multi\u2010Omics Study Integrating Metallomics, Transcriptomics and Cellular Analyses"],"prefix":"10.1111","author":[{"given":"Yichen","family":"Lin","sequence":"first","affiliation":[{"name":"Department of Epidemiology and Health Statistics, School of Public Health Fujian Medical University  Fuzhou 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