{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,11]],"date-time":"2026-10-11T23:45:38Z","timestamp":1791762338154,"version":"4.3.4"},"reference-count":33,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,10,11]],"date-time":"2026-10-11T00:00:00Z","timestamp":1791676800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,10,11]],"date-time":"2026-10-11T00:00:00Z","timestamp":1791676800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"publisher","award":["RS\u20102025\u201000522998"],"award-info":[{"award-number":["RS\u20102025\u201000522998"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Clinic Periodontology"],"abstract":"ABSTRACT<\/jats:title>\n \n Aim<\/jats:title>\n To investigate the microbial network signatures associated with outcomes following surgical therapy of peri\u2010implantitis.<\/jats:p>\n <\/jats:sec>\n \n Materials and Methods<\/jats:title>\n \n Microbiological data from a 1\u2010year randomised controlled trial of 28 patients (28 implants) were analysed. Based on a composite outcome (probing depth \u2264\u20095\u2009mm, minimal bleeding on probing and no further marginal bone loss) at 1\u2009year, implant sites were categorised into a successful (\n n<\/jats:italic>\n \u2009=\u200910) or a non\u2010successful group (\n n<\/jats:italic>\n \u2009=\u200918). Peri\u2010implant crevicular fluid was collected at baseline and at 3, 6 and 12\u2009months and analysed using 16S rRNA gene sequencing.\n <\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n A significant compositional shift from baseline to 1\u2009year was observed in the successful group (\n p<\/jats:italic>\n \u2009=\u20090.001), with a significant increase in\n \n Rothia aeria<\/jats:italic>\n <\/jats:styled-content>\n (\n p<\/jats:italic>\n \u2009=\u20090.048),\n \n Haemophilus parainfluenzae<\/jats:italic>\n <\/jats:styled-content>\n (\n p<\/jats:italic>\n \u2009=\u20090.033) and\n Neisseria oralis<\/jats:italic>\n (\n p<\/jats:italic>\n \u2009=\u20090.025) over time. Network analysis revealed that the successful group exhibited a\n \n Porphyromonas gingivalis<\/jats:italic>\n <\/jats:styled-content>\n \u2010associated module enriched with high\u2010centrality commensals at baseline, and significantly greater network edge density (\n p<\/jats:italic>\n \u2009=\u20090.005) and connectivity (\n p<\/jats:italic>\n \u2009=\u20090.018) at 1\u2009year. Spearman correlation analyses at 1\u2009year identified significant negative correlations between\n \n P. gingivalis<\/jats:italic>\n <\/jats:styled-content>\n and commensals including\n \n H. parainfluenzae<\/jats:italic>\n <\/jats:styled-content>\n ,\n Streptococcus<\/jats:italic>\n species and\n Veillonella<\/jats:italic>\n undefined (\n p<\/jats:italic>\n \u2009<\u20090.05).\n <\/jats:p>\n <\/jats:sec>\n \n Conclusion<\/jats:title>\n In this exploratory secondary analysis of a small cohort, favourable outcomes of peri\u2010implantitis therapy were associated with increased microbial network connectivity and commensal centrality.<\/jats:p>\n <\/jats:sec>\n \n Trial Registration<\/jats:title>\n Korean Clinical Research Information Service number KCT0007900<\/jats:p>\n <\/jats:sec>","DOI":"10.1111\/jcpe.70214","type":"journal-article","created":{"date-parts":[[2026,10,11]],"date-time":"2026-10-11T23:25:35Z","timestamp":1791761135000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Microbial Network Signatures Associated With Outcomes of Peri\u2010Implantitis Therapy"],"prefix":"10.1111","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6207-8036","authenticated-orcid":false,"given":"Seung\u2010Hyun","family":"Park","sequence":"first","affiliation":[{"name":"Department of Periodontology Research Institute for Periodontal Regeneration, Yonsei University College of Dentistry Seoul Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-1124-7929","authenticated-orcid":false,"given":"Da\u2010Mi","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Periodontology Research Institute for Periodontal Regeneration, Yonsei University College of Dentistry Seoul Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-3741-3007","authenticated-orcid":false,"given":"Seulbin","family":"Im","sequence":"additional","affiliation":[{"name":"Department of Periodontology Research Institute for Periodontal Regeneration, Yonsei University College of Dentistry Seoul Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5823-6591","authenticated-orcid":false,"given":"Eduardo H.","family":"de Souza Oliveira","sequence":"additional","affiliation":[{"name":"Department of Oral Medicine, Infection, and Immunity Harvard School of Dental Medicine Boston Massachusetts USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7037-1163","authenticated-orcid":false,"given":"Ignacio","family":"Sanz\u2010Martin","sequence":"additional","affiliation":[{"name":"Department of Periodontology Research Institute for Periodontal Regeneration, Yonsei University College of Dentistry Seoul Republic of Korea"},{"name":"Section of Graduate Periodontology University Complutense of Madrid Madrid Spain"},{"name":"Private Practice Lausanne Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3698-4772","authenticated-orcid":false,"given":"Ignacio","family":"Sanz\u2010Sanchez","sequence":"additional","affiliation":[{"name":"Section of Graduate Periodontology University Complutense of Madrid Madrid Spain"},{"name":"ETEP (Etiology and Therapy of Periodontal and Peri\u2010Implant Diseases) Research Group, Faculty of Dentistry University Complutense of Madrid Madrid Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1133-6074","authenticated-orcid":false,"given":"Jan","family":"Derks","sequence":"additional","affiliation":[{"name":"Department of Periodontology Sahlgrenska Academy, University of Gothenburg Gothenburg Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2293-3392","authenticated-orcid":false,"given":"Magda","family":"Feres","sequence":"additional","affiliation":[{"name":"Department of Oral Medicine, Infection, and Immunity Harvard School of Dental Medicine Boston Massachusetts USA"},{"name":"Dental Research Division Guarulhos University Guarulhos Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6906-7209","authenticated-orcid":false,"given":"Jae\u2010Kook","family":"Cha","sequence":"additional","affiliation":[{"name":"Department of Periodontology Research Institute for Periodontal Regeneration, Yonsei University College of Dentistry Seoul Republic of Korea"},{"name":"Department of Oral Medicine, Infection, and Immunity Harvard School of Dental Medicine Boston Massachusetts USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,10,11]]},"reference":[{"issue":"1","key":"e_1_2_10_2_1","doi-asserted-by":"crossref","first-page":"12","DOI":"10.1038\/s41522-024-00482-z","article-title":"Favorable Subgingival Plaque Microbiome Shifts Are Associated With Clinical Treatment for Peri\u2010Implant Diseases","volume":"10","author":"Bazzani D.","year":"2024","journal-title":"npj Biofilms and Microbiomes"},{"issue":"1","key":"e_1_2_10_3_1","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1177\/0022034520949851","article-title":"Microbial Community\u2010Driven Etiopathogenesis of Peri\u2010Implantitis","volume":"100","author":"Belibasakis G.","year":"2021","journal-title":"Journal of Dental Research"},{"key":"e_1_2_10_4_1","doi-asserted-by":"crossref","DOI":"10.3389\/fcimb.2025.1543100","article-title":"Linking Peri\u2010Implantitis to Microbiome Changes in Affected Implants, Healthy Implants, and Saliva: A Cross\u2010Sectional Pilot Study","volume":"15","author":"Bessa L. J.","year":"2025","journal-title":"Frontiers in Cellular and Infection Microbiology"},{"issue":"8","key":"e_1_2_10_5_1","doi-asserted-by":"crossref","first-page":"962","DOI":"10.1038\/ismej.2010.30","article-title":"Bacterial Diversity in the Oral Cavity of 10 Healthy Individuals","volume":"4","author":"Bik E. M.","year":"2010","journal-title":"ISME Journal"},{"issue":"11","key":"e_1_2_10_6_1","doi-asserted-by":"crossref","first-page":"1176","DOI":"10.1111\/clr.14153","article-title":"Microbiota Associated With Peri\u2010Implantitis\u2014A Systematic Review With Meta\u2010Analyses","volume":"34","author":"Carvalho \u00c9. B.","year":"2023","journal-title":"Clinical Oral Implants Research"},{"issue":"1","key":"e_1_2_10_7_1","first-page":"S1","article-title":"A New Classification Scheme for Periodontal and Peri\u2010Implant Diseases and Conditions\u2013Introduction and Key Changes From the 1999 Classification","volume":"89","author":"Caton J. G.","year":"2018","journal-title":"Journal of Periodontology"},{"issue":"5","key":"e_1_2_10_8_1","doi-asserted-by":"crossref","first-page":"1210","DOI":"10.1038\/s41396-017-0037-1","article-title":"Oral Microbiota of Periodontal Health and Disease and Their Changes After Nonsurgical Periodontal Therapy","volume":"12","author":"Chen C.","year":"2018","journal-title":"ISME Journal"},{"issue":"1","key":"e_1_2_10_9_1","doi-asserted-by":"crossref","first-page":"167","DOI":"10.1111\/prd.12290","article-title":"Restorative Design and Associated Risks for Peri\u2010Implant Diseases","volume":"81","author":"Dixon D. R.","year":"2019","journal-title":"Periodontology 2000"},{"issue":"1","key":"e_1_2_10_10_1","doi-asserted-by":"crossref","first-page":"240","DOI":"10.1186\/s12915-021-01169-z","article-title":"Long\u2010Term Dynamics of the Human Oral Microbiome During Clinical Disease Progression","volume":"19","author":"Duran\u2010Pinedo A.","year":"2021","journal-title":"BMC Biology"},{"issue":"1","key":"e_1_2_10_11_1","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1038\/s41368-025-00367-7","article-title":"Community Dynamics During De Novo Colonization of the Nascent Peri\u2010Implant Sulcus","volume":"17","author":"Dutra T. P.","year":"2025","journal-title":"International Journal of Oral Science"},{"issue":"12","key":"e_1_2_10_12_1","doi-asserted-by":"crossref","first-page":"1662","DOI":"10.1111\/jcpe.70024","article-title":"Profiling the Bacterial Microbiome Across Peri\u2010Implant Conditions","volume":"52","author":"Espinoza\u2010Arrue J.","year":"2025","journal-title":"Journal of Clinical Periodontology"},{"issue":"1","key":"e_1_2_10_13_1","doi-asserted-by":"crossref","first-page":"182","DOI":"10.1111\/prd.12358","article-title":"Did Omics Change Periodontal Therapy?","volume":"85","author":"Feres M.","year":"2021","journal-title":"Periodontology 2000"},{"issue":"7","key":"e_1_2_10_14_1","doi-asserted-by":"crossref","first-page":"999","DOI":"10.1111\/jcpe.14121","article-title":"Shotgun Metagenomics Identifies in a Cross\u2010Sectional Setting Improved Plaque Microbiome Biomarkers for Peri\u2010Implant Diseases","volume":"52","author":"Ghensi P.","year":"2025","journal-title":"Journal of Clinical Periodontology"},{"issue":"1","key":"e_1_2_10_15_1","doi-asserted-by":"crossref","DOI":"10.1038\/s41522-020-00155-7","article-title":"Strong Oral Plaque Microbiome Signatures for Dental Implant Diseases Identified by Strain\u2010Resolution Metagenomics","volume":"6","author":"Ghensi P.","year":"2020","journal-title":"npj Biofilms and Microbiomes"},{"issue":"6","key":"e_1_2_10_16_1","doi-asserted-by":"crossref","first-page":"477","DOI":"10.1016\/j.tim.2016.02.010","article-title":"Dancing With the Stars: How Choreographed Bacterial Interactions Dictate Nososymbiocity and Give Rise to Keystone Pathogens, Accessory Pathogens, and Pathobionts","volume":"24","author":"Hajishengallis G.","year":"2016","journal-title":"Trends in Microbiology"},{"issue":"4","key":"e_1_2_10_17_1","doi-asserted-by":"crossref","first-page":"710","DOI":"10.1111\/cid.13183","article-title":"Implant Prosthodontic Design as a Predisposing or Precipitating Factor for Peri\u2010Implant Disease: A Review","volume":"25","author":"Hamilton A.","year":"2023","journal-title":"Clinical Implant Dentistry and Related Research"},{"key":"e_1_2_10_18_1","doi-asserted-by":"crossref","first-page":"4","DOI":"10.1111\/jcpe.13823","article-title":"Prevention and Treatment of Peri\u2010Implant Diseases\u2014The EFP S3 Level Clinical Practice Guideline","volume":"50","author":"Herrera D.","year":"2023","journal-title":"Journal of Clinical Periodontology"},{"key":"e_1_2_10_19_1","doi-asserted-by":"crossref","DOI":"10.1016\/j.micpath.2021.105100","article-title":"The Temporal Shift of Peri\u2010Implant Microbiota During the Biofilm Formation and Maturation in a Canine Model","volume":"158","author":"Jiang Q.","year":"2021","journal-title":"Microbial Pathogenesis"},{"issue":"12","key":"e_1_2_10_20_1","doi-asserted-by":"crossref","first-page":"1498","DOI":"10.1111\/jcpe.13023","article-title":"The Severity of Human Peri\u2010Implantitis Lesions Correlates With the Level of Submucosal Microbial Dysbiosis","volume":"45","author":"Kroeger A.","year":"2018","journal-title":"Journal of Clinical Periodontology"},{"issue":"5","key":"e_1_2_10_21_1","doi-asserted-by":"crossref","first-page":"425","DOI":"10.1111\/j.1600-051X.2012.01856.x","article-title":"Pyrosequencing Reveals Unique Microbial Signatures Associated With Healthy and Failing Dental Implants","volume":"39","author":"Kumar P. S.","year":"2012","journal-title":"Journal of Clinical Periodontology"},{"issue":"10","key":"e_1_2_10_22_1","doi-asserted-by":"crossref","first-page":"1066","DOI":"10.1902\/jop.2017.170123","article-title":"Microbiome and Microbial Biofilm Profiles of Peri\u2010Implantitis: A Systematic Review","volume":"88","author":"Lafaurie G. I.","year":"2017","journal-title":"Journal of Periodontology"},{"issue":"11","key":"e_1_2_10_23_1","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pcbi.1009442","article-title":"Multivariable Association Discovery in Population\u2010Scale Meta\u2010Omics Studies","volume":"17","author":"Mallick H.","year":"2021","journal-title":"PLoS Computational Biology"},{"issue":"2","key":"e_1_2_10_24_1","doi-asserted-by":"crossref","first-page":"238","DOI":"10.1111\/jre.12708","article-title":"Pyrosequencing of the Subgingival Microbiome in Peri\u2010Implantitis After Non\u2010Surgical Mechanical Debridement Therapy","volume":"55","author":"Nie J.","year":"2020","journal-title":"Journal of Periodontal Research"},{"issue":"2","key":"e_1_2_10_25_1","doi-asserted-by":"crossref","first-page":"244","DOI":"10.1111\/jcpe.70056","article-title":"Implantoplasty vs. Rotating Titanium Brushes in the Surgical Treatment of Peri\u2010Implantitis: A 1\u2010Year Randomised Controlled Clinical Trial","volume":"53","author":"Park S. H.","year":"2026","journal-title":"Journal of Clinical Periodontology"},{"issue":"11","key":"e_1_2_10_26_1","doi-asserted-by":"crossref","first-page":"1295","DOI":"10.1902\/jop.2016.160184","article-title":"The Current Weight of Evidence of the Microbiologic Profile Associated With Peri\u2010Implantitis: A Systematic Review","volume":"87","author":"P\u00e9rez\u2010Chaparro P. J.","year":"2016","journal-title":"Journal of Periodontology"},{"issue":"4","key":"e_1_2_10_27_1","doi-asserted-by":"crossref","first-page":"371","DOI":"10.1177\/0022034517742139","article-title":"Resilience of the Oral Microbiota in Health: Mechanisms That Prevent Dysbiosis","volume":"97","author":"Rosier B.","year":"2018","journal-title":"Journal of Dental Research"},{"issue":"12","key":"e_1_2_10_28_1","doi-asserted-by":"crossref","first-page":"1274","DOI":"10.1111\/jcpe.12788","article-title":"Exploring the Microbiome of Healthy and Diseased Peri\u2010Implant Sites Using Illumina Sequencing","volume":"44","author":"Sanz\u2010Martin I.","year":"2017","journal-title":"Journal of Clinical Periodontology"},{"issue":"2","key":"e_1_2_10_29_1","doi-asserted-by":"crossref","first-page":"172","DOI":"10.1111\/clr.13880","article-title":"Microbial Profiles of Peri\u2010Implant Mucositis and Peri\u2010Implantitis: Submucosal Microbial Dysbiosis Correlates With Disease Severity","volume":"33","author":"Shi Y.","year":"2022","journal-title":"Clinical Oral Implants Research"},{"key":"e_1_2_10_30_1","doi-asserted-by":"crossref","DOI":"10.3389\/fcimb.2022.1091938","article-title":"Shift in the Submucosal Microbiome of Diseased Peri\u2010Implant Sites After Non\u2010Surgical Mechanical Debridement Treatment","volume":"12","author":"Sun F.","year":"2023","journal-title":"Frontiers in Cellular and Infection Microbiology"},{"issue":"1","key":"e_1_2_10_31_1","doi-asserted-by":"crossref","DOI":"10.1111\/cid.13423","article-title":"Submucosal Microbiome Profiles in Paired and Unpaired Samples From Healthy and Peri\u2010Implantitis Dental Implants","volume":"27","author":"Tocarruncho O. I.","year":"2025","journal-title":"Clinical Implant Dentistry and Related Research"},{"issue":"4","key":"e_1_2_10_32_1","doi-asserted-by":"crossref","first-page":"657","DOI":"10.1111\/jre.13269","article-title":"Microbiome of Periodontitis and Peri\u2010Implantitis Before and After Therapy: Long\u2010Read 16S rRNA Gene Amplicon Sequencing","volume":"59","author":"Yu P. S.","year":"2024","journal-title":"Journal of Periodontal Research"},{"issue":"8","key":"e_1_2_10_33_1","doi-asserted-by":"crossref","first-page":"760","DOI":"10.1111\/clr.13459","article-title":"Intra\u2010Oral Single\u2010Site Comparisons of Periodontal and Peri\u2010Implant Microbiota in Health and Disease","volume":"30","author":"Yu X. L.","year":"2019","journal-title":"Clinical Oral Implants Research"},{"key":"e_1_2_10_34_1","doi-asserted-by":"crossref","DOI":"10.3389\/fmicb.2021.785191","article-title":"Periodontal and Peri\u2010Implant Microbiome Dysbiosis Is Associated With Alterations in the Microbial Community Structure and Local Stability","volume":"12","author":"Zhang Y.","year":"2022","journal-title":"Frontiers in Microbiology"}],"container-title":["Journal of Clinical Periodontology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jcpe.70214","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/jcpe.70214","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jcpe.70214","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,11]],"date-time":"2026-10-11T23:25:40Z","timestamp":1791761140000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/jcpe.70214"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,10,11]]},"references-count":33,"alternative-id":["10.1111\/jcpe.70214"],"URL":"https:\/\/doi.org\/10.1111\/jcpe.70214","archive":["Portico"],"relation":{},"ISSN":["0303-6979","1600-051X"],"issn-type":[{"value":"0303-6979","type":"print"},{"value":"1600-051X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,10,11]]},"assertion":[{"value":"2026-05-11","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-09-28","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-10-11","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"jcpe.70214"}}