{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,24]],"date-time":"2026-10-24T01:42:26Z","timestamp":1792806146853,"version":"4.5.0"},"reference-count":62,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T00:00:00Z","timestamp":1792713600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T00:00:00Z","timestamp":1792713600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100000925","name":"National Health and Medical Research Council","doi-asserted-by":"publisher","award":["2034591"],"award-info":[{"award-number":["2034591"]}],"id":[{"id":"10.13039\/501100000925","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000925","name":"National Health and Medical Research Council","doi-asserted-by":"publisher","award":["1195451"],"award-info":[{"award-number":["1195451"]}],"id":[{"id":"10.13039\/501100000925","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004799","name":"Australian Dental Research Foundation","doi-asserted-by":"publisher","award":["PJ\u20100000042"],"award-info":[{"award-number":["PJ\u20100000042"]}],"id":[{"id":"10.13039\/501100004799","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100010631","name":"Australian Periodontology Research Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100010631","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Clinic Periodontology"],"abstract":"ABSTRACT<\/jats:title>\n \n Aim<\/jats:title>\n To evaluate the longitudinal changes in salivary host and non\u2010host extracellular vesicles (EVs) following non\u2010surgical periodontal therapy (NSPT).<\/jats:p>\n <\/jats:sec>\n \n Materials and Methods<\/jats:title>\n Unstimulated saliva and plaque (from a deep pocket) were collected from 25 Stage III\/IV periodontitis patients at baseline and at 3 and 6 months post NSPT. Host salivary EVs and non\u2010host EVs were isolated using immunoaffinity, and size\u2010exclusion chromatography, respectively. Host EV cytokines were assessed by multiplex flow cytometry, and non\u2010host EVs, saliva and plaque were profiled by 16S rRNA and fungal internal transcribed spacer (ITS) sequencing.<\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n Periodontal parameters significantly decreased at 3 and 6\u2009months post NSPT (vs. baseline), accompanied by increased host\u2010EVs associated cytokines interleukin\u20104 (IL\u20104), IL\u201012 and IL\u20109 (not whole saliva). At both timepoints, bacterial EVs also declined, including the genera\n Filifactor<\/jats:italic>\n ,\n Parvimonas<\/jats:italic>\n and\n Lentimicrobium<\/jats:italic>\n and the species\n \n Fusobacterium nucleatum<\/jats:italic>\n <\/jats:styled-content>\n ,\n \n Filifactor alocis<\/jats:italic>\n <\/jats:styled-content>\n ,\n \n Tannerella forsythia<\/jats:italic>\n <\/jats:styled-content>\n ,\n \n Treponema denticola<\/jats:italic>\n <\/jats:styled-content>\n ,\n \n Porphyromonas gingivalis<\/jats:italic>\n <\/jats:styled-content>\n ,\n \n P.\u2009endodontalis<\/jats:italic>\n <\/jats:styled-content>\n ,\n \n P.\u2009micra<\/jats:italic>\n <\/jats:styled-content>\n and\n Lachnospiraceae bacterium;<\/jats:italic>\n similar reductions were observed in plaque and saliva taxa, including\n Treponema<\/jats:italic>\n ,\n Tannerella<\/jats:italic>\n ,\n Parvimonas<\/jats:italic>\n ,\n \n T.\u2009forsythia<\/jats:italic>\n <\/jats:styled-content>\n ,\n \n T.\u2009socranskii<\/jats:italic>\n <\/jats:styled-content>\n and\n \n P.\u2009micra<\/jats:italic>\n <\/jats:styled-content>\n . At 6\u2009months, fungal EVs from\n Malassezia arunalokei<\/jats:italic>\n were decreased and the fungal\n \n Candida parapsilosis<\/jats:italic>\n <\/jats:styled-content>\n EVs were increased.\n <\/jats:p>\n <\/jats:sec>\n \n Conclusions<\/jats:title>\n NSPT led to changes in salivary host and non\u2010host EV cargoes, suggesting that they may serve as a non\u2010invasive tool for disease treatment monitoring in periodontitis.<\/jats:p>\n <\/jats:sec>","DOI":"10.1111\/jcpe.70216","type":"journal-article","created":{"date-parts":[[2026,10,24]],"date-time":"2026-10-24T01:05:38Z","timestamp":1792803938000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["An Exploratory Longitudinal Analysis of Salivary Host, Bacterial and Fungal Extracellular Vesicles Following 6\u2010Month Non\u2010Surgical Periodontal Therapy in Periodontitis"],"prefix":"10.1111","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7551-3685","authenticated-orcid":false,"given":"Chun","family":"Liu","sequence":"first","affiliation":[{"name":"Centre for Orofacial Regeneration, Rehabilitation and Reconstruction (COR3), Epigenetics Nanodiagnostic and Therapeutic Group, School of Dentistry The University of Queensland Brisbane Queensland Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jenny","family":"Wang","sequence":"additional","affiliation":[{"name":"Centre for Orofacial Regeneration, Rehabilitation and Reconstruction (COR3), Epigenetics Nanodiagnostic and Therapeutic Group, School of Dentistry The University of Queensland Brisbane Queensland Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4424-8767","authenticated-orcid":false,"given":"Andrew","family":"Lai","sequence":"additional","affiliation":[{"name":"Translational Extracellular Vesicles in Obstetrics and Gynae\u2010Oncology Group, The University of Queensland Frazer Institute, Royal Brisbane and Women's Hospital, Faculty of Medicine The University of Queensland Brisbane Queensland Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4474-0046","authenticated-orcid":false,"given":"Carlos","family":"Salomon","sequence":"additional","affiliation":[{"name":"Translational Extracellular Vesicles in Obstetrics and Gynae\u2010Oncology Group, The University of Queensland Frazer Institute, Royal Brisbane and Women's Hospital, Faculty of Medicine The University of Queensland Brisbane Queensland Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0816-538X","authenticated-orcid":false,"given":"Chaminda Jayampath","family":"Seneviratne","sequence":"additional","affiliation":[{"name":"Centre for Orofacial Regeneration, Rehabilitation and Reconstruction (COR3), Epigenetics Nanodiagnostic and Therapeutic Group, School of Dentistry The University of Queensland Brisbane Queensland Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5339-0936","authenticated-orcid":false,"given":"Sa\u0161o","family":"Ivanovski","sequence":"additional","affiliation":[{"name":"Centre for Orofacial Regeneration, Rehabilitation and Reconstruction (COR3), Epigenetics Nanodiagnostic and Therapeutic Group, School of Dentistry The University of Queensland Brisbane Queensland Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7546-1635","authenticated-orcid":false,"given":"Pingping","family":"Han","sequence":"additional","affiliation":[{"name":"Centre for Orofacial Regeneration, Rehabilitation and Reconstruction (COR3), Epigenetics Nanodiagnostic and Therapeutic Group, School of Dentistry The University of Queensland Brisbane Queensland 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