{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,8]],"date-time":"2026-05-08T23:40:17Z","timestamp":1778283617027,"version":"3.51.4"},"reference-count":41,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2018,10,24]],"date-time":"2018-10-24T00:00:00Z","timestamp":1540339200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Nature Science Foundation of China","doi-asserted-by":"crossref","award":["81400323"],"award-info":[{"award-number":["81400323"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"name":"the Key Scientific Research Project of Henan Province College","award":["15A320029"],"award-info":[{"award-number":["15A320029"]}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Diabetes Invest"],"published-print":{"date-parts":[[2019,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Aims\/Introduction<\/jats:title><jats:p>Diabetes is an important risk factor for atherosclerotic disease. The initiating factor of atherosclerosis is local endothelial cell injury. The arachidonic acid metabolite, 12(S)\u2010hydroxyeicosatetraenoic acid (12[S]\u2010HETE), might be involved in this process. In recent years, some studies have discussed the effect of 12(S)\u2010HETE on vascular endothelial cell function. In the present study, we investigated the effect of 12(S)\u2010HETE on vascular endothelial cell function in high\u2010glucose conditions and the mechanisms involved.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and Methods<\/jats:title><jats:p>Human umbilical vein endothelial cells were cultured in conventional M199 medium and high\u2010glucose M199 medium. Human umbilical vein endothelial cells were stimulated with 12(S)\u2010HETE and cinnamyl\u20103,4\u2010dihydroxy\u2010\u03b1\u2010cyanocinnamate (a 12\/15\u2010lipoxygenases inhibitor). A type\u00a01 diabetes mellitus model was established in C57BL\/6 or 12\/15\u2010lipoxygenases knockout mice with streptozotocin. Aortic tissue was harvested for subsequent testing. The transmembrane transport of dextran and human acute monocytic leukaemia cell line (THP\u20101) cells was measured. The adherens junction protein, IkB\u03b1, nuclear factor kappa\u00a0Bp65 (P65), intercellular adhesion molecule\u00a01 and vascular cell adhesion protein\u00a01 expression and phosphorylation, and the binding\/dissociation of endothelial cell components were observed.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Transendothelial migration of dextran and THP\u20101 cells was significantly increased by stimulation of human umbilical vein endothelial cell monolayers with high glucose and 12(S)\u2010HETE (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.05). High glucose and 12(S)\u2010HETE altered the vascular endothelial cadherin and \u03b2\u2010catenin phosphorylation level, and promoted the dissociation of \u03b2\u2010catenin and vascular endothelial cadherin. Expression levels of P\u2010Ikb\u03b1, P\u2010P65, intercellular adhesion molecule\u00a01 and vascular cell adhesion protein\u00a01 were elevated in high glucose and 12(S)\u2010HETE treated cells and diabetic mice compared with controls (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.05).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The lipoxygenases metabolite, 12(S)\u2010HETE, can impair vascular endothelial permeability by altering adherens junction phosphorylation levels, and affecting the binding and dissociation of its components in high\u2010glucose conditions.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jdi.12941","type":"journal-article","created":{"date-parts":[[2018,9,25]],"date-time":"2018-09-25T05:43:49Z","timestamp":1537854229000},"page":"639-649","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["12(S)\u2010hydroxyeicosatetraenoic acid impairs vascular endothelial permeability by altering adherens junction phosphorylation levels and affecting the binding and dissociation of its components in high glucose\u2010induced vascular injury"],"prefix":"10.1111","volume":"10","author":[{"given":"Xiaofang","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Cardiology First Affiliated Hospital College of Medicine Zhengzhou University Zhengzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lu","family":"Gao","sequence":"additional","affiliation":[{"name":"Department of Cardiology First Affiliated Hospital College of Medicine Zhengzhou University Zhengzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lili","family":"Xiao","sequence":"additional","affiliation":[{"name":"Department of Cardiology First Affiliated Hospital College of Medicine Zhengzhou University Zhengzhou 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