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Hepatic \u03b17nAchR action is impeded in obesity and insulin resistance. In the present study, using \u03b17nAchR knockout (\u03b17<jats:styled-content style=\"fixed-case\">KO<\/jats:styled-content>) mice, we elucidated the effect of \u03b17nAchR deficiency on <jats:styled-content style=\"fixed-case\">NASH<\/jats:styled-content>\u2010related inflammation and fibrosis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and Methods<\/jats:title><jats:p>\u03b17<jats:styled-content style=\"fixed-case\">KO<\/jats:styled-content> mice were fed an atherogenic high\u2010fat diet (<jats:styled-content style=\"fixed-case\">AD<\/jats:styled-content>) for 32\u00a0weeks or methionine\/choline\u2010deficient diet (<jats:styled-content style=\"fixed-case\">MCD<\/jats:styled-content>) for 6\u00a0weeks, both of which induce <jats:styled-content style=\"fixed-case\">NASH<\/jats:styled-content>. Mice were then examined for the degree of <jats:styled-content style=\"fixed-case\">NASH<\/jats:styled-content>\u2010related inflammation\u00a0and fibrosis by hepatic gene expression analysis and Sirius red histological staining.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Hepatic triglyceride accumulation and elevated plasma transaminase levels were observed in both <jats:styled-content style=\"fixed-case\">AD<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">MCD<\/jats:styled-content> mice, but the plasma transaminase level increase was higher in \u03b17<jats:styled-content style=\"fixed-case\">KO<\/jats:styled-content> mice than in control mice. \u03b17<jats:styled-content style=\"fixed-case\">KO<\/jats:styled-content> mice fed an <jats:styled-content style=\"fixed-case\">AD<\/jats:styled-content> showed significant upregulation of the <jats:italic>Col1a1<\/jats:italic> gene encoding alpha\u20101 type\u00a0I collagen, which is involved in liver fibrosis, and the <jats:italic>Ccl2<\/jats:italic> gene encoding C\u2010C motif chemokine ligand\u00a02, a pro\u2010inflammatory chemokine; \u03b17<jats:styled-content style=\"fixed-case\">KO<\/jats:styled-content> mice fed an <jats:styled-content style=\"fixed-case\">MCD<\/jats:styled-content> had significant upregulation of the <jats:italic>Col1a1<\/jats:italic> gene and the <jats:italic>Tnf<\/jats:italic> gene, an inflammatory cytokine. Histological analysis showed that <jats:styled-content style=\"fixed-case\">AD<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">MCD<\/jats:styled-content> exacerbated liver fibrosis in \u03b17<jats:styled-content style=\"fixed-case\">KO<\/jats:styled-content> mice.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The results of this study suggest that \u03b17nAchR deficiency exacerbates hepatic inflammation and fibrosis in a diet\u2010induced mouse model of <jats:styled-content style=\"fixed-case\">NASH<\/jats:styled-content>.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jdi.12964","type":"journal-article","created":{"date-parts":[[2018,10,28]],"date-time":"2018-10-28T04:24:17Z","timestamp":1540700657000},"page":"659-666","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":38,"title":["Nicotinic alpha\u20107 acetylcholine receptor deficiency exacerbates hepatic inflammation and fibrosis in a mouse model of non\u2010alcoholic steatohepatitis"],"prefix":"10.1111","volume":"10","author":[{"given":"Kumi","family":"Kimura","sequence":"first","affiliation":[{"name":"Metabolism and Nutrition Research Unit Institute for Frontier Science Initiative Kanazawa University Kanazawa Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9757-9669","authenticated-orcid":false,"given":"Yuka","family":"Inaba","sequence":"additional","affiliation":[{"name":"Metabolism and Nutrition Research Unit Institute for Frontier Science Initiative Kanazawa University Kanazawa Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hitoshi","family":"Watanabe","sequence":"additional","affiliation":[{"name":"Metabolism and Nutrition Research Unit Institute for Frontier Science Initiative Kanazawa University Kanazawa Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Toshiya","family":"Matsukawa","sequence":"additional","affiliation":[{"name":"Department of Molecular Metabolic Regulation Diabetes Research Center Research Institute National Center for Global Health and Medicine Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michihiro","family":"Matsumoto","sequence":"additional","affiliation":[{"name":"Department of Molecular Metabolic Regulation Diabetes Research Center Research Institute National Center for Global Health and Medicine Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroshi","family":"Inoue","sequence":"additional","affiliation":[{"name":"Metabolism and Nutrition Research Unit Institute for Frontier Science Initiative Kanazawa University Kanazawa 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