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However, the function of <jats:italic>Kbtbd11<\/jats:italic> during adipogenesis is unknown. The aim of the present study was to investigate the role of <jats:italic>Kbtbd11<\/jats:italic> in the differentiation of 3T3\u2010L1 preadipocytes.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and Methods<\/jats:title><jats:p>For the fasting\u2013refeeding protocol, mice were subjected to fasting for 24\u00a0h, followed by a chow diet for 12\u00a0h. Adenovirus infection methods were used to examine the effect of <jats:italic>Kbtbd11<\/jats:italic>, and 3T3\u2010L1 cells were analyzed with Oil Red O staining and real\u2010time polymerase chain reaction.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The white adipose tissue expression of <jats:italic>Kbtbd11<\/jats:italic> messenger ribonucleic acid (mRNA) was significantly higher in the re\u2010fed state than in the fasted state. <jats:italic>Kbtbd11<\/jats:italic> mRNA levels were markedly increased in epididymal white adipose tissue of diet\u2010induced obesity mice compared with those in the mice fed a chow diet. In addition, <jats:italic>Kbtbd11<\/jats:italic> mRNA expression was increased in a differentiation\u2010dependent manner in 3T3\u2010L1 cells. Knockdown of <jats:italic>Kbtbd11<\/jats:italic> mRNA through the infection with adenoviral vectors remarkably inhibited triglyceride accumulation and adipocyte differentiation in 3T3\u2010L1 cells. In contrast, the overexpression of <jats:italic>Kbtbd11<\/jats:italic> promoted the differentiation of 3T3\u2010L1 adipocytes.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The present findings show that <jats:italic>Kbtbd11<\/jats:italic> expression might be involved in nutritional regulation and is increased in obese adipose tissue. In addition, <jats:italic>Kbtbd11<\/jats:italic> appears to be required for the differentiation of adipocytes in 3T3<jats:italic>\u2010<\/jats:italic>L1 cells. Collectively, these results show a novel link between the expression of <jats:italic>Kbtbd11<\/jats:italic> and fat accumulation, and suggest that <jats:italic>Kbtbd11<\/jats:italic> is a new therapeutic target for obesity.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jdi.12995","type":"journal-article","created":{"date-parts":[[2018,12,24]],"date-time":"2018-12-24T16:35:13Z","timestamp":1545669313000},"page":"925-932","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["<i>Kbtbd11<\/i> gene expression in adipose tissue increases in response to feeding and affects adipocyte differentiation"],"prefix":"10.1111","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5640-0818","authenticated-orcid":false,"given":"Kazuhisa","family":"Watanabe","sequence":"first","affiliation":[{"name":"Division of Human Genetics Center for Molecular Medicine Jichi Medical University Shimotsuke Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ken","family":"Yoshida","sequence":"additional","affiliation":[{"name":"Division of Human Genetics Center for Molecular Medicine Jichi Medical University Shimotsuke Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sadahiko","family":"Iwamoto","sequence":"additional","affiliation":[{"name":"Division of Human Genetics Center for Molecular Medicine Jichi Medical University Shimotsuke 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