{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T21:26:36Z","timestamp":1790630796488,"version":"4.1.0"},"reference-count":29,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2020,5,9]],"date-time":"2020-05-09T00:00:00Z","timestamp":1588982400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Diabetes Invest"],"published-print":{"date-parts":[[2020,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Aims\/Objective<\/jats:title><jats:p>The development of type\u00a02 diabetes is a result of insulin resistance in various tissues, including skeletal muscle and liver. Apolipoprotein\u00a0M (ApoM) plays an important role in the function of high\u2010density lipoprotein, and also affects hepatic lipid and glucose metabolism. In this study, we aimed to investigate whether ApoM overexpression modulates glucose metabolism and improves insulin sensitivity.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and Methods<\/jats:title><jats:p>The Goto\u2010Kakizaki (GK) rats were transfected with adeno\u2010associated virus (AAV) encoding rat ApoM gene or control blank. The oral glucose tolerance test (OGTT) and hyperinsulinemic\u2010euglycemic clamp (HEC) experiment were used to assess the insulin sensitivity of GK rats.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The results show that ApoM messenger ribonucleic acid and protein were significantly overexpressed in the pancreatic tissues. Overexpression of ApoM decreased fasting blood glucose and random blood glucose, improved glucose tolerance, and increased bodyweight and insulin levels in GK rats. The glucose infusion rate of rats in the AAV encoding rat ApoM gene group during HEC test was 1.04\u2010, 1.23\u2010 and 1.95\u2010fold higher than that in the AAV control blank group at 1\u20133\u00a0weeks after injection of AAV, respectively. A Wes\u2010ProteinSimple assay and quantification was carried out to assess phosphorylated protein kinase\u00a0B\/protein kinase\u00a0B protein levels in the muscle tissues of ApoM\u2010overexpressing GK rats, and they were found to be higher than those of the control group at the seventh week after AAV injection.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>ApoM overexpression through adeno\u2010associated virus gene transfer might improve insulin secretion and insulin sensitivity in GK rats.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jdi.13261","type":"journal-article","created":{"date-parts":[[2020,4,3]],"date-time":"2020-04-03T15:08:55Z","timestamp":1585926535000},"page":"1150-1158","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Apolipoprotein\u00a0M overexpression through adeno\u2010associated virus gene transfer improves insulin secretion and insulin sensitivity in Goto\u2010Kakizaki rats"],"prefix":"10.1111","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9258-786X","authenticated-orcid":false,"given":"Yang","family":"Yu","sequence":"first","affiliation":[{"name":"Comprehensive Laboratory the Third Affiliated Hospital of Soochow University Changzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jun","family":"Zhang","sequence":"additional","affiliation":[{"name":"Comprehensive Laboratory the Third Affiliated Hospital of Soochow University Changzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuang","family":"Yao","sequence":"additional","affiliation":[{"name":"Comprehensive Laboratory the Third Affiliated Hospital of Soochow University Changzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lili","family":"Pan","sequence":"additional","affiliation":[{"name":"Comprehensive Laboratory the Third Affiliated Hospital of Soochow University Changzhou 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