{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,18]],"date-time":"2026-01-18T12:24:09Z","timestamp":1768739049610,"version":"3.49.0"},"reference-count":32,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2020,11,27]],"date-time":"2020-11-27T00:00:00Z","timestamp":1606435200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001648","name":"European Foundation for the Study of Diabetes","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001648","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Diabetes Invest"],"published-print":{"date-parts":[[2021,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Aims\/Introduction<\/jats:title><jats:p>Type\u00a01 diabetes mellitus is a T\u00a0cell\u2010mediated autoimmune disease. However, the determination of the autoimmune status of type\u00a01 diabetes mellitus relies on islet autoantibodies (Abs), as T\u2010cell assay is not routinely carried out. This study aimed to investigate the diagnostic value of combined assay of islet antigen\u2010specific T\u00a0cells and Abs in type\u00a01 diabetes mellitus patients.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and Methods<\/jats:title><jats:p>A total of 54 patients with type\u00a01 diabetes mellitus and 56 healthy controls were enrolled. Abs against glutamic acid decarboxylase (GAD), islet antigen\u20102 and zinc transporter 8 were detected by radioligand assay. Interferon\u2010\u03b3\u2010secreting T cells responding to glutamic acid decarboxylase\u00a065 and C\u2010peptide (CP) were measured by enzyme\u2010linked immunospot.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The positive rate for T\u2010cell responses was significantly higher in patients with type\u00a01 diabetes mellitus than that in controls (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.001). The combined positive rate of Abs and T\u2010cell assay was significantly higher than that of Abs assay alone (85.2% vs 64.8%, <jats:italic>P<\/jats:italic>\u00a0=\u00a00.015). A significant difference in fasting CP level was found between the T<jats:sup>+<\/jats:sup> and T<jats:sup>\u2013<\/jats:sup> groups (0.07\u00a0\u00b1\u00a00.05 vs 0.11\u00a0\u00b1\u00a00.09\u00a0nmol\/L, <jats:italic>P<\/jats:italic>\u00a0=\u00a00.033). Furthermore, levels of fasting CP and postprandial CP were both lower in the Ab<jats:sup>\u2212<\/jats:sup>T<jats:sup>+<\/jats:sup> group than the Ab<jats:sup>\u2212<\/jats:sup>T<jats:sup>\u2212<\/jats:sup> group (fasting CP 0.06\u00a0\u00b1\u00a00.05 vs 0.16\u00a0\u00b1\u00a00.12\u00a0nmol\/L, <jats:italic>P<\/jats:italic>\u00a0=\u00a00.041; postprandial CP 0.12\u00a0\u00b1\u00a00.13 vs 0.27\u00a0\u00b1\u00a00.12\u00a0nmol\/L, <jats:italic>P<\/jats:italic>\u00a0=\u00a00.024).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Enzyme\u2010linked immunospot assays in combination with Abs detection could improve the diagnostic sensitivity of autoimmune diabetes.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jdi.13440","type":"journal-article","created":{"date-parts":[[2020,10,16]],"date-time":"2020-10-16T17:20:55Z","timestamp":1602868855000},"page":"963-969","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Diagnostic value of combined islet antigen\u2010reactive T\u00a0cells and autoantibodies assays for type\u00a01 diabetes mellitus"],"prefix":"10.1111","volume":"12","author":[{"given":"Wei","family":"Tang","sequence":"first","affiliation":[{"name":"National Clinical Research Center for Metabolic Diseases, Key Laboratory of Diabetes Immunology, Ministry of Education, and Department of Metabolism and Endocrinology, The Second Xiangya Hospital of Central South University Changsha Hunan China"},{"name":"Department of Metabolism and Endocrinology The First People\u2019s Hospital of Huaihua Huaihua Hunan 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Clinical Research Center for Metabolic Diseases, Key Laboratory of Diabetes Immunology, Ministry of Education, and Department of Metabolism and Endocrinology, The Second Xiangya Hospital of Central South University Changsha Hunan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8959-1139","authenticated-orcid":false,"given":"Lin","family":"Yang","sequence":"additional","affiliation":[{"name":"National Clinical Research Center for Metabolic Diseases, Key Laboratory of Diabetes Immunology, Ministry of Education, and Department of Metabolism and Endocrinology, The Second Xiangya Hospital of Central South University Changsha Hunan 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