{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T18:11:54Z","timestamp":1788545514375,"version":"build-2803163510"},"reference-count":51,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2020,11,30]],"date-time":"2020-11-30T00:00:00Z","timestamp":1606694400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Diabetes Invest"],"published-print":{"date-parts":[[2021,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Aims\/Introduction<\/jats:title><jats:p>Abnormalities in Ca<jats:sup>2+<\/jats:sup> signaling have a key role in hemodynamic dysfunction in diabetic heart. The purpose of this study was to explore the effects of streptozotocin (STZ)\u2010induced diabetes on Ca<jats:sup>2+<\/jats:sup> signaling in epicardial (EPI) and endocardial (ENDO) cells of the left ventricle after 5\u20136\u00a0months of STZ injection.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and Methods<\/jats:title><jats:p>Whole\u2010cell patch clamp was used to measure the L\u2010type Ca<jats:sup>2+<\/jats:sup> channel (LTCC) and Na<jats:sup>+<\/jats:sup>\/Ca<jats:sup>2+<\/jats:sup> exchanger currents. Fluorescence photometry techniques were used to measure intracellular free Ca<jats:sup>2+<\/jats:sup> concentration.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Although the LTCC current was not significantly altered, the amplitude of Ca<jats:sup>2+<\/jats:sup> transients increased significantly in EPI\u2010STZ and ENDO\u2010STZ compared with controls. Time to peak LTCC current, time to peak Ca<jats:sup>2+<\/jats:sup> transient, time to half decay of LTCC current and time to half decay of Ca<jats:sup>2+<\/jats:sup> transients were not significantly changed in EPI\u2010STZ and ENDO\u2010STZ myocytes compared with controls. The Na<jats:sup>+<\/jats:sup>\/Ca<jats:sup>2+<\/jats:sup> exchanger current was significantly smaller in EPI\u2010STZ and in ENDO\u2010STZ compared with controls.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>STZ\u2010induced diabetes resulted in an increase in amplitude of Ca<jats:sup>2+<\/jats:sup> transients in EPI and ENDO myocytes that was independent of the LTCC current. Such an effect can be attributed, at least in part, to the dysfunction of the Na<jats:sup>+<\/jats:sup>\/Ca<jats:sup>2+<\/jats:sup> exchanger. Additional studies are warranted to improve our understanding of the regional impact of diabetes on Ca<jats:sup>2+<\/jats:sup> signaling, which will facilitate the discovery of new targeted treatments for diabetic cardiomyopathy.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jdi.13451","type":"journal-article","created":{"date-parts":[[2020,10,28]],"date-time":"2020-10-28T16:10:40Z","timestamp":1603901440000},"page":"493-500","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Calcium signaling in endocardial and epicardial ventricular myocytes from streptozotocin\u2010induced diabetic rats"],"prefix":"10.1111","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8338-7655","authenticated-orcid":false,"given":"Lina T","family":"Al Kury","sequence":"first","affiliation":[{"name":"Department of Health Sciences College of Natural and Health Sciences Zayed University Abu Dhabi United Arab 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