{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,9]],"date-time":"2026-07-09T05:34:13Z","timestamp":1783575253295,"version":"3.55.0"},"reference-count":39,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2021,2,22]],"date-time":"2021-02-22T00:00:00Z","timestamp":1613952000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100007129","name":"Natural Science Foundation of Shandong Province","doi-asserted-by":"publisher","award":["ZR201702160271"],"award-info":[{"award-number":["ZR201702160271"]}],"id":[{"id":"10.13039\/501100007129","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100014857","name":"National Natural Science Foundation of China-Guangdong Joint Fund","doi-asserted-by":"publisher","award":["81702538"],"award-info":[{"award-number":["81702538"]}],"id":[{"id":"10.13039\/501100014857","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Diabetes Invest"],"published-print":{"date-parts":[[2021,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Aims\/Introduction<\/jats:title><jats:p>Differentially expressed microribonucleic acids (miRNAs) in the placenta and circulating exosomes are of diagnostic value for gestational diabetes mellitus (GDM). In a cross\u2010sectional study, we identified miRNAs expressed both in the placenta and circulating exosomes of pregnant women with GDM, and estimated their diagnostic value.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and Methods<\/jats:title><jats:p>Next\u2010generation sequencing was used to identify miRNAs in the placenta that were differentially expressed between GDM and normal glucose tolerance pregnancies. Quantitative polymerase chain reaction was used to validate the identified targets. Western blot and transmission electron microscopy were used to validate exosomes. Univariate logistic regression analysis was used to establish diagnostic models based on miRNAs expression, and the diagnostic value was estimated using the receiver operator characteristic curve.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>We identified 157 dysregulated miRNAs in the placental tissue obtained from GDM pregnancies. Of these, miRNA\u2010125b was downregulated (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.001), whereas miRNA\u2010144 was upregulated (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.001). The patterns of these two miRNAs remained the same in circulating exosomes from GDM pregnancies (all <jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.001). miRNA\u2010144 levels in the circulating exosomes negatively correlated with body mass index both before pregnancy (<jats:italic>P<\/jats:italic>\u00a0=\u00a00.018) and before delivery (<jats:italic>P<\/jats:italic>\u00a0=\u00a00.039), and positively correlated with blood glucose at 1\u00a0h, estimated using the oral glucose tolerance test (<jats:italic>P<\/jats:italic>\u00a0=\u00a00.044). The area under curve for the established diagnostic model was 0.898, which was higher than blood glucose levels at 0\u00a0h.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>These findings suggest that miRNA\u2010125b and miRNA\u2010144 are consistently dysregulated in circulating exosomes and the placenta from GDM pregnancies, and are of excellent diagnostic value for GDM.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jdi.13493","type":"journal-article","created":{"date-parts":[[2021,1,11]],"date-time":"2021-01-11T01:14:03Z","timestamp":1610327643000},"page":"1490-1500","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":33,"title":["Diagnostic value of dysregulated microribonucleic acids in the placenta and circulating exosomes in gestational diabetes mellitus"],"prefix":"10.1111","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0421-2804","authenticated-orcid":false,"given":"Lei","family":"Zhang","sequence":"first","affiliation":[{"name":"Department of Obstetrics The Second Hospital Cheeloo College of Medicine Shandong University Ji\u2019nan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ting","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Pharmacy Jinan Infectious Disease Hospital Cheeloo College of Medicine Shandong University Ji\u2019nan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daoxu","family":"Sun","sequence":"additional","affiliation":[{"name":"Office of Heze Health Association Heze China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guanghui","family":"Cheng","sequence":"additional","affiliation":[{"name":"Central Research Laboratory The Second Hospital Cheeloo College of Medicine Shandong University Ji\u2019nan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hanxiao","family":"Ren","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory Medicine The Second Hospital Cheeloo College of Medicine Shandong University Ji\u2019nan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haijie","family":"Hong","sequence":"additional","affiliation":[{"name":"Department of Obstetrics The Second Hospital Cheeloo College of Medicine Shandong University Ji\u2019nan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liyu","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Obstetrics The Second Hospital Cheeloo College of Medicine Shandong University Ji\u2019nan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xue","family":"Jiao","sequence":"additional","affiliation":[{"name":"School of Medicine Cheeloo College of Medicine Shandong University Ji\u2019nan 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