{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,9]],"date-time":"2026-06-09T06:50:49Z","timestamp":1780987849854,"version":"3.54.1"},"reference-count":38,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2021,9,8]],"date-time":"2021-09-08T00:00:00Z","timestamp":1631059200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004663","name":"Ministry of Science and Technology, Taiwan","doi-asserted-by":"publisher","award":["MOST 109\u20102314\u2010B\u2010182A\u2010128"],"award-info":[{"award-number":["MOST 109\u20102314\u2010B\u2010182A\u2010128"]}],"id":[{"id":"10.13039\/501100004663","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Diabetes Invest"],"published-print":{"date-parts":[[2022,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Aims\/Introduction:<\/jats:title><jats:p>To investigate the association between specific bacterial pathogens and treatment outcome in patients with limb\u2010threatening diabetic foot infection (LT\u2010DFI).<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and Methods:<\/jats:title><jats:p>Consecutive patients treated for LT\u2010DFI in a major diabetic foot center in Taiwan were analyzed between the years 2014 and 2017. Patients with positive wound culture results at first aid were enrolled. Clinical factors, laboratory data, and wound culture results were compared. Lower\u2010extremity amputations and in\u2010hospital mortality were defined as a poor outcome.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results:<\/jats:title><jats:p>Among the 558 patients, 272 (48.7%) patients had lower extremity amputation and 22 (3.9%) patients had in\u2010hospital mortality. Gram\u2010negative bacterial (GNB) infection was the independent factor following factors adjustment. When all the 31 microorganisms were analyzed, only<jats:italic>E.\u2009coli<\/jats:italic>(adjusted odds ratio [aOR], 3.01; 95% CI, 1.60\u20135.65),<jats:italic>Proteus<\/jats:italic>spp. (aOR, 2.99; 95% CI, 1.69\u20135.29), and<jats:italic>Pseudomonas aeruginosa<\/jats:italic>(aOR, 2.00; 95% CI 1.20\u20133.32) were associated with poor outcome. The analysis of specific GNB species in association with major\u2010 or minor\u2010 amputation have been reported. No specific pathogen was associated with cause of death in patients with mortality within 30\u2009days. The antimicrobial\u2010resistant strains were not associated with a poor treatment outcome.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions:<\/jats:title><jats:p>The presence of GNB was associated with limb amputations. This study provides insight into more timely and appropriate management of the diabetic foot infection.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jdi.13649","type":"journal-article","created":{"date-parts":[[2021,8,21]],"date-time":"2021-08-21T20:02:40Z","timestamp":1629576160000},"page":"336-343","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Impact of wound microbiology on limb preservation in patients with diabetic foot infection"],"prefix":"10.1111","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9243-6586","authenticated-orcid":false,"given":"Shih\u2010Yuan","family":"Hung","sequence":"first","affiliation":[{"name":"Division of Endocrinology and Metabolism Department of Internal Medicine Chang Gung Memorial Hospital Taoyuan City Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cheng\u2010Hsun","family":"Chiu","sequence":"additional","affiliation":[{"name":"Division of Pediatric Infectious Diseases Department of Pediatrics Chang Gung Memorial Hospital Taoyuan City Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chung\u2010Huei","family":"Huang","sequence":"additional","affiliation":[{"name":"Division of Endocrinology and Metabolism Department of Internal Medicine Chang Gung Memorial Hospital Taoyuan City Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cheng\u2010Wei","family":"Lin","sequence":"additional","affiliation":[{"name":"Division of Endocrinology and Metabolism Department of Internal Medicine Chang Gung Memorial Hospital Taoyuan City Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiun\u2010Ting","family":"Yeh","sequence":"additional","affiliation":[{"name":"Division of Trauma Plastic Surgery Department of Plastic and Reconstructive Surgery Chang Gung Memorial Hospital Chang Gung University Taoyuan City Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hui\u2010Mei","family":"Yang","sequence":"additional","affiliation":[{"name":"Division of Endocrinology and Metabolism Department of Internal Medicine Chang Gung Memorial Hospital Taoyuan City Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4501-6140","authenticated-orcid":false,"given":"Yu\u2010Yao","family":"Huang","sequence":"additional","affiliation":[{"name":"Division of Endocrinology and Metabolism Department of Internal Medicine Chang Gung Memorial Hospital Taoyuan City Taiwan"},{"name":"College of Medicine Chang Gung University Taoyuan City Taiwan"},{"name":"Department of Medical Nutrition Therapy Chang Gung Memorial Hospital Taoyuan City 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