{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,14]],"date-time":"2025-05-14T01:25:26Z","timestamp":1747185926379,"version":"3.40.5"},"reference-count":9,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2022,5,26]],"date-time":"2022-05-26T00:00:00Z","timestamp":1653523200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100009337","name":"Saitama Medical University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100009337","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Diabetes Invest"],"published-print":{"date-parts":[[2022,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:title>Aims\/Introduction<\/jats:title><jats:p>Some women develop type\u20091 diabetes during pregnancy or immediately after delivery. However, the underlying pathophysiology remains largely unknown, probably because of the lack of a suitable animal model. In this study, we administered pregnant NOD mice with an anti\u2010CD25 antibody to reduce regulatory T\u2009cells along with poly I:C and examined the onset of diabetes.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and Methods<\/jats:title><jats:p>Anti\u2010CD25 antibody and poly I:C were intraperitoneally administered to mated female NOD mice. Mice delivered within 3\u2009weeks after the treatment, and the onset of diabetes during pregnancy or within 6\u2009weeks after delivery was examined. Some mice were killed 1\u2009week after treatment, and their spleen and pancreas were excised to examine the expression levels of cytokines and for histological examination.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Half of the mice treated with anti\u2010CD25 antibody plus poly I:C developed diabetes, as compared with none of the poly I:C\u2010injected mice (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.05). The ratios of interleukin\u201018\/forkhead box P3 and granzyme\u2009B\/forkhead box P3 were higher in the pancreas of anti\u2010CD25 antibody plus poly I:C\u2010treated mice than in the pancreas of control mice. The insulitis score decreased in the pancreas of anti\u2010CD25 antibody plus poly I:C\u2010injected pregnant NOD mice.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>We describe the use of anti\u2010CD25 antibody to reduce regulatory T\u2009cells and poly I:C as a Toll\u2010like receptor\u20093 stimulator to mimic viral infection in a pregnant NOD mouse, which can be used as a model of \u2018pregnancy\u2010related\u2019 type\u20091 diabetes.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jdi.13829","type":"journal-article","created":{"date-parts":[[2022,5,9]],"date-time":"2022-05-09T15:55:26Z","timestamp":1652111726000},"page":"1489-1495","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Combination of <scp>anti\u2010CD25<\/scp> antibody and poly I:C treatment in pregnant <scp>NOD<\/scp> mice may be used as \u2018pregnancy\u2010related\u2019 type\u20091 diabetes model"],"prefix":"10.1111","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3954-4983","authenticated-orcid":false,"given":"Akira","family":"Shimada","sequence":"first","affiliation":[{"name":"Department of Endocrinology and Diabetes Saitama Medical University  Saitama Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kyoko","family":"Toda","sequence":"additional","affiliation":[{"name":"Department of Endocrinology and Diabetes Saitama Medical University  Saitama Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ikuo","family":"Inoue","sequence":"additional","affiliation":[{"name":"Department of Endocrinology and Diabetes Saitama Medical University  Saitama Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Taketo","family":"Yamada","sequence":"additional","affiliation":[{"name":"Department of Pathology Saitama Medical University  Saitama Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1524-6751","authenticated-orcid":false,"given":"Yoichi","family":"Oikawa","sequence":"additional","affiliation":[{"name":"Department of Endocrinology and Diabetes Saitama Medical University  Saitama Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2022,5,26]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0168-8227(03)00147-5"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1000837"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2007.12.191"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/imm.12065"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.171.11.5865"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1006\/jaut.2002.0599"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.2337\/db15-1331"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm.2173"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1196\/annals.1447.031"}],"container-title":["Journal of Diabetes Investigation"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jdi.13829","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/jdi.13829","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jdi.13829","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,18]],"date-time":"2023-08-18T01:38:25Z","timestamp":1692322705000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/jdi.13829"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,5,26]]},"references-count":9,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2022,9]]}},"alternative-id":["10.1111\/jdi.13829"],"URL":"https:\/\/doi.org\/10.1111\/jdi.13829","archive":["Portico"],"relation":{},"ISSN":["2040-1116","2040-1124"],"issn-type":[{"type":"print","value":"2040-1116"},{"type":"electronic","value":"2040-1124"}],"subject":[],"published":{"date-parts":[[2022,5,26]]},"assertion":[{"value":"2019-12-29","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-05-05","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-05-26","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}