{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T09:56:18Z","timestamp":1790848578672,"version":"4.1.0"},"reference-count":75,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2026,7,29]],"date-time":"2026-07-29T00:00:00Z","timestamp":1785283200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2026,7,29]],"date-time":"2026-07-29T00:00:00Z","timestamp":1785283200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Diabetes Invest"],"published-print":{"date-parts":[[2026,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Purpose<\/jats:title>\n                    <jats:p>This study aimed to investigate the molecular mechanism by which the miR\u2010145\u20105p\/NRF2\/SLC7A11 axis regulates ferroptosis\u2010mediated renal tubular epithelial cell (RTEC) injury in diabetic nephropathy (DN).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Male C57BL\/KsJ\u2010db\/db mice were used as a murine DN model and treated with a miR\u2010145\u20105p antagomir, adeno\u2010associated virus carrying NRF2 short hairpin RNA (NRF2\u2010shRNA), or the ferroptosis inducer (Erastin) or inhibitor (Ferrostatin\u20101). Renal function was assessed by measuring the urinary albumin\u2010to\u2010creatinine ratio, serum creatinine, and blood urea nitrogen. Renal histopathological injury and fibrosis were evaluated.\n                      <jats:italic>In vitro<\/jats:italic>\n                      , mouse RTECs (TCMK\u20101) were exposed to high glucose (HG) and transfected with a miR\u2010145\u20105p antagomir or small interfering RNAs targeting NRF2 or SLC7A11. The interaction between miR\u2010145\u20105p and NRF2 was validated using a dual\u2010luciferase assay, while the binding of NRF2 to the SLC7A11 promoter was confirmed by chromatin immunoprecipitation.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>miR\u2010145\u20105p expression was upregulated in the kidneys of DN mice. Inhibition of miR\u2010145\u20105p reduced ferroptosis, attenuated renal histological injury and fibrosis, and reversed epithelial\u2010to\u2010mesenchymal transition. These protective effects were partially abolished by Erastin treatment. Similarly, miR\u2010145\u20105p inhibition suppressed HG\u2010induced ferroptosis in TCMK\u20101 cells. Mechanistically, miR\u2010145\u20105p directly targeted NRF2, and NRF2 bound to the promoter region of SLC7A11. Knockdown of either NRF2 or SLC7A11 partially reversed the anti\u2010ferroptotic effects of miR\u2010145\u20105p suppression. Furthermore, NRF2 silencing partially abrogated the protective effects of miR\u2010145\u20105p inhibition in DN mice, while Ferrostatin\u20101 partially rescued renal tissue damage induced by NRF2 knockdown.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Suppression of miR\u2010145\u20105p alleviates renal injury in DN by inhibiting ferroptosis through activation of the NRF2\/SLC7A11 axis.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/jdi.70397","type":"journal-article","created":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T06:21:52Z","timestamp":1785392512000},"page":"1667-1685","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["The\n                    <scp>miR<\/scp>\n                    \u2010145\u20105p\/\n                    <scp>NRF2<\/scp>\n                    \/\n                    <scp>SLC7A11<\/scp>\n                    axis regulates ferroptosis\u2010mediated renal tubular epithelial cell injury in diabetic nephropathy"],"prefix":"10.1111","volume":"17","author":[{"given":"Ganlin","family":"Wu","sequence":"first","affiliation":[{"name":"Department of Nephrology Hubei University of Science and Technology  Xianning China"},{"name":"National Demonstration Center for Experimental (General practice) Education, Xianning Medical College Hubei University of Science and Technology  Xianning China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu","family":"Fang","sequence":"additional","affiliation":[{"name":"Department of Nephrology Xianning Central Hospital (The First Affiliated Hospital of Hubei University of Science and Technology)  Xianning China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meirong","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Geriatrics The Second Affiliated Hospital of Hubei University of Science and Technology  Xianning China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-9344-627X","authenticated-orcid":false,"given":"Zuopeng","family":"Lu","sequence":"additional","affiliation":[{"name":"Department of Surgery People's Hospital of Tongcheng County  Xianning China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu","family":"Cao","sequence":"additional","affiliation":[{"name":"Department of Pharmacy Xianning Central Hospital (The First Affiliated Hospital of Hubei University of Science and Technology)  Xianning China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yana","family":"Gao","sequence":"additional","affiliation":[{"name":"Department of Nephrology Hubei University of Science and Technology  Xianning 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