{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T09:55:12Z","timestamp":1790848512816,"version":"4.1.0"},"reference-count":32,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2026,7,23]],"date-time":"2026-07-23T00:00:00Z","timestamp":1784764800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2026,7,23]],"date-time":"2026-07-23T00:00:00Z","timestamp":1784764800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Diabetes Invest"],"published-print":{"date-parts":[[2026,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Aim<\/jats:title>\n                    <jats:p>\n                      To ascertain the expression status and clinical performance of miR\u2010224\u20105p in diabetic nephropathy (DN), alongside its molecular mechanism of regulating mesangial cell injury by targeting\n                      <jats:italic>phosphatase and tensin homolog<\/jats:italic>\n                      (\n                      <jats:italic>PTEN<\/jats:italic>\n                      ).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Serum miR\u2010224\u20105p and\n                      <jats:italic>PTEN<\/jats:italic>\n                      mRNA expression were detected in DN patients. Logistic regression was used to analyze independent risk indexes for the poor clinical outcomes in DN. Functional gain\/loss and rescue experiments were performed. Target relationships were validated through dual\u2010luciferase reporter assays.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Serum miR\u2010224\u20105p expression was significantly increased in DN patients (\n                      <jats:italic>P<\/jats:italic>\n                      \u2009&lt;\u20090.001). It displayed moderate diagnostic performance for discriminating DN from T2DM (AUC\u2009=\u20090.796), and good predictive value (AUC\u2009=\u20090.833) for predicting 2\u2010year adverse renal outcomes. MiR\u2010224\u20105p level was significantly higher in patients with an unfavorable prognosis than those with a favorable prognosis (\n                      <jats:italic>P<\/jats:italic>\n                      \u2009&lt;\u20090.001). Elevated miR\u2010224\u20105p was identified as an independent risk factor for eGFR decline \u226530% (\n                      <jats:italic>P<\/jats:italic>\n                      \u2009=\u20090.001), but this finding was not applicable to end\u2010stage renal disease (ESRD) due to insufficient case numbers for multivariate analysis. MiR\u2010224\u20105p inhibition protected high\u2010glucose mesangial cells via\n                      <jats:italic>PTEN<\/jats:italic>\n                      upregulation, an effect reversed by si\u2010\n                      <jats:italic>PTEN<\/jats:italic>\n                      .\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Increased miR\u2010224\u20105p expression may serve as a potential circulating biomarker for predicting eGFR decline in DN patients, but these findings are preliminary and require validation in independent cohorts using standardized exogenous spike\u2010in normalization before clinical application. The independence of this association was only demonstrated for the eGFR decline \u226530% endpoint and not for ESRD due to limited statistical power.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/jdi.70401","type":"journal-article","created":{"date-parts":[[2026,7,23]],"date-time":"2026-07-23T11:13:11Z","timestamp":1784805191000},"page":"1744-1758","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Targeting the\n                    <scp>miR<\/scp>\n                    \u2010224\u20105p\/\n                    <scp>PTEN<\/scp>\n                    pathway: A\u00a0previously under\u2010characterized strategy to attenuate mesangial cell injury and predict prognosis in diabetic nephropathy"],"prefix":"10.1111","volume":"17","author":[{"ORCID":"https:\/\/orcid.org\/0009-0000-1129-4815","authenticated-orcid":false,"given":"Yanxia","family":"Gao","sequence":"first","affiliation":[{"name":"Department of Nephrology Qilu Hospital of Shandong University (Qingdao)  Qingdao China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaohui","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Nephrology Qilu Hospital of Shandong University (Qingdao)  Qingdao China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peng","family":"Lu","sequence":"additional","affiliation":[{"name":"Department of Nephrology Qilu Hospital of Shandong University (Qingdao)  Qingdao China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiang","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Nephrology Qilu Hospital of Shandong University (Qingdao)  Qingdao 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