{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,31]],"date-time":"2026-08-31T05:16:50Z","timestamp":1788153410847,"version":"build-2803163510"},"reference-count":45,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,8,30]],"date-time":"2026-08-30T00:00:00Z","timestamp":1788048000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"},{"start":{"date-parts":[[2026,8,30]],"date-time":"2026-08-30T00:00:00Z","timestamp":1788048000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100003995","name":"Natural Science Foundation of Anhui Province","doi-asserted-by":"publisher","award":["2108085MH269"],"award-info":[{"award-number":["2108085MH269"]}],"id":[{"id":"10.13039\/501100003995","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Diabetes Invest"],"abstract":"ABSTRACT<\/jats:title>\n \n Aims<\/jats:title>\n To investigate the role of RNA N6\u2010methyladenosine (m6A) methyltransferase Wilms tumor 1\u2010associated protein (WTAP) in negative pressure wound therapy (NPWT) for diabetic foot ulcers (DFU).<\/jats:p>\n <\/jats:sec>\n \n Materials and Methods<\/jats:title>\n We enrolled 46 DFU patients and 16 non\u2010diabetic chronic ulcer patients treated with NPWT. Total m6A levels and the expression of methyltransferases (METTL3, METTL14, and WTAP) in wound margin tissues were measured before and after NPWT. High glucose\u2010induced HaCaT and HDF cell models, alongside an in vitro negative pressure system, were used to evaluate the effect of negative pressure on WTAP expression and the impact of WTAP alteration on cellular functions.<\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n Before NPWT, METTL3, METTL14, WTAP and m6A levels were significantly lower in DFU patients than in the non\u2010diabetic chronic ulcer patients. After one week of NPWT, these parameters increased significantly in both groups, and a positive correlation was observed between WTAP and m6A levels. Notably, the increase in WTAP expression exhibited a significant positive correlation with the 4\u2010week healing outcome in DFU patients. In vitro, negative pressure significantly reversed the high glucose\u2010induced WTAP downregulation in both HaCaT and HDF cells, while WTAP knockdown profoundly impaired their proliferation and migration.<\/jats:p>\n <\/jats:sec>\n \n Conclusions<\/jats:title>\n NPWT is associated with improved DFU healing and the restoration of WTAP\u2010m6A levels, and its effect may be related to negative pressure promoting WTAP expression to enhance wound cell proliferation and migration.<\/jats:p>\n <\/jats:sec>","DOI":"10.1111\/jdi.70430","type":"journal-article","created":{"date-parts":[[2026,8,31]],"date-time":"2026-08-31T04:24:47Z","timestamp":1788150287000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["WTAP<\/scp>\n \u2010mediated\n m6A RNA<\/scp>\n methylation promotes diabetic foot ulcer healing under negative pressure wound therapy"],"prefix":"10.1111","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7508-0680","authenticated-orcid":false,"given":"Yutong","family":"Li","sequence":"first","affiliation":[{"name":"The First Affiliated Hospital of Anhui Medical University Hefei Anhui China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenhua","family":"Chen","sequence":"additional","affiliation":[{"name":"The First Affiliated Hospital of Anhui Medical University Hefei Anhui China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianhao","family":"Lu","sequence":"additional","affiliation":[{"name":"The First Affiliated Hospital of Anhui Medical University Hefei Anhui China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoyue","family":"Lin","sequence":"additional","affiliation":[{"name":"The First Affiliated Hospital of Anhui Medical University Hefei Anhui 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