{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,27]],"date-time":"2026-02-27T02:51:01Z","timestamp":1772160661060,"version":"3.50.1"},"reference-count":19,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2012,12,20]],"date-time":"2012-12-20T00:00:00Z","timestamp":1355961600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["30470104"],"award-info":[{"award-number":["30470104"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Acad Dermatol Venereol"],"published-print":{"date-parts":[[2013,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    <jats:bold>Background\u2002<\/jats:bold>\n                    The dimorphic fungus\n                    <jats:italic>Sporothrix schenckii<\/jats:italic>\n                    is the etiological agent of sporotrichosis, an important cutaneous mycosis with a worldwide distribution. At present, it is challenging to rapidly discover and identify\n                    <jats:italic>Sporothrix schenckii<\/jats:italic>\n                    in biopsy tissues nowadays.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Aims\u2002<\/jats:bold>\n                    To explore new methods for rapid diagnosis of sporotrichosis.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Materials and Methods\u2002<\/jats:bold>\n                    We screened specific primers for\n                    <jats:italic>Sporothrix schenckii<\/jats:italic>\n                    using 50 clinical isolates from patients with sporotrichosis. DNA was extracted from the lesions of 30 cases of clinically suspected sporotrichosis using the Graham\u00a0s method of CTAB and amplified by PCR using the screened specific primers.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Results\u2002<\/jats:bold>\n                    The primer S2\u2010R2 was applicable for the identification of\n                    <jats:italic>S. schenckii<\/jats:italic>\n                    from different geographic areas and clinical types with high specificity and sensitivity. Twenty\u2010five out of the thirty cases (83.3%) amplified using the primer S2\u2010R2 showed positive bands. Further positive bands were observed in 95.6% of cases tested positive by fungal culture.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Conclusions\u2002<\/jats:bold>\n                    Using the PCR technique and specific primers, we developed a new diagnostic method that can rapidly diagnose sporotrichosis with tissues obtained from clinical biopsies.\n                  <\/jats:p>","DOI":"10.1111\/jdv.12030","type":"journal-article","created":{"date-parts":[[2012,12,21]],"date-time":"2012-12-21T21:45:50Z","timestamp":1356126350000},"page":"1491-1497","source":"Crossref","is-referenced-by-count":23,"title":["Rapid identification of\n                    <i>Sporothrix schenckii<\/i>\n                    in biopsy tissue by PCR"],"prefix":"10.1111","volume":"27","author":[{"given":"X.","family":"Liu","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Z.","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B.","family":"Hou","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D.","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T.","family":"Sun","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"F.","family":"Li","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H.","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.","family":"Han","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2012,12,20]]},"reference":[{"key":"e_1_2_6_2_2","first-page":"216","article-title":"Sporotrichosis: 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