{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,17]],"date-time":"2026-06-17T03:07:18Z","timestamp":1781665638845,"version":"3.54.5"},"reference-count":47,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2013,3,21]],"date-time":"2013-03-21T00:00:00Z","timestamp":1363824000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Lebanese National Council for Scientific Research Organization"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Acad Dermatol Venereol"],"published-print":{"date-parts":[[2014,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p><jats:styled-content style=\"fixed-case\">Proto\u2010oncogene B\u2010Raf (BRAF)<\/jats:styled-content> mutation rates have been reported in nevi and melanomas of homogeneous Caucasian cohorts.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objective<\/jats:title><jats:p>To study the demographics of <jats:styled-content style=\"fixed-case\">BRAF<\/jats:styled-content> mutations in dysplastic nevi of populations with differing potential solar <jats:styled-content style=\"fixed-case\">UV<\/jats:styled-content> radiation exposure.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Extended <jats:styled-content style=\"fixed-case\">BRAF<\/jats:styled-content> testing for 9 mutations in 125 dysplastic nevi from 101 patients, derived from populations with differing potential <jats:styled-content style=\"fixed-case\">UV<\/jats:styled-content> radiation exposure rates (Lebanon and Saudi Arabia), was performed. Clinical and microscopic parameters were recorded.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p><jats:styled-content style=\"fixed-case\">BRAF<\/jats:styled-content> mutation status was carried out for 101\/125 (80.8%) cases with an overall mutation rate of 62.4% (63\/101). V600E (c.1799T\u00a0&gt;\u00a0A) was the predominant mutation, found in 61\/63 (96.8%) cases. <jats:styled-content style=\"fixed-case\">BRAF<\/jats:styled-content> mutation rate differed significantly by potential <jats:styled-content style=\"fixed-case\">UV<\/jats:styled-content> radiation exposure (Lebanon: 53.4%, Saudi Arabia: 74.4%, <jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.05). A 43.8% discordant mutation rate (7\/16 patients) was found in patients with multiple nevi, including 2 patients with different <jats:styled-content style=\"fixed-case\">BRAF<\/jats:styled-content> mutations. Microscopic examination subdivided the dysplasia into mild (<jats:italic>n<\/jats:italic>\u00a0=\u00a024), moderate (<jats:italic>n<\/jats:italic>\u00a0=\u00a060) and severe (<jats:italic>n<\/jats:italic>\u00a0=\u00a041) with trunk predominance (72.8%). Higher rates of pigment in the stratum corneum were identified in Saudi Arabia (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.05). No statistical significant increase in <jats:styled-content style=\"fixed-case\">BRAF<\/jats:styled-content> mutation rate was noted with advanced architectural and cytological atypia. Parameters associated with a negative <jats:styled-content style=\"fixed-case\">BRAF<\/jats:styled-content> mutation status included upper extremity location, regression, cohesiveness and presence of suprabasal melanocytes (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.05). Positive <jats:styled-content style=\"fixed-case\">BRAF<\/jats:styled-content> mutation status was reasonably predicted by multivariate binary logistic regression by 2 independent predictors: Geographic location and compound nevus type.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>In our Near Eastern cohort, the <jats:styled-content style=\"fixed-case\">BRAF<\/jats:styled-content> mutation rate varied significantly by geographic location. In patients with multiple dysplastic nevi examined, discordant <jats:styled-content style=\"fixed-case\">BRAF<\/jats:styled-content> mutation status potentially negates an underlying constitutional predilection.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jdv.12148","type":"journal-article","created":{"date-parts":[[2013,3,28]],"date-time":"2013-03-28T10:09:15Z","timestamp":1364465355000},"page":"615-625","source":"Crossref","is-referenced-by-count":11,"title":["BRAF mutational epidemiology in dysplastic nevi: Does different solar UV radiation exposure matter?"],"prefix":"10.1111","volume":"28","author":[{"given":"M.","family":"Saroufim","sequence":"first","affiliation":[{"name":"Department of Pathology American University of Beirut Medical Center Beirut Lebanon"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Novy","sequence":"additional","affiliation":[{"name":"ViennaLab Diagnostics GmbH Vienna Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"Taraif","sequence":"additional","affiliation":[{"name":"Saad Specialist Hospital Al Khobar Kingdom of Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R.H.","family":"Habib","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine Outcomes Research Unit American University of Beirut Medical Center Beirut Lebanon"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Loya","sequence":"additional","affiliation":[{"name":"Shaukat Khanum Memorial Center Lahore Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B.","family":"Rauscher","sequence":"additional","affiliation":[{"name":"ViennaLab Diagnostics GmbH Vienna Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G.","family":"Kriegsh\u00e4user","sequence":"additional","affiliation":[{"name":"ViennaLab Diagnostics GmbH Vienna Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C.","family":"Oberkanins","sequence":"additional","affiliation":[{"name":"ViennaLab Diagnostics GmbH Vienna Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"I.","family":"Khalifeh","sequence":"additional","affiliation":[{"name":"Department of Pathology American University of Beirut Medical Center Beirut Lebanon"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2013,3,21]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1136\/jcp.2004.019422"},{"key":"e_1_2_7_3_1","doi-asserted-by":"crossref","first-page":"995","DOI":"10.1001\/archderm.1991.01680060069006","article-title":"Dysplastic nevi as risk markers of sporadic (nonfamilial) melanoma. 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