{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,29]],"date-time":"2026-07-29T11:51:34Z","timestamp":1785325894379,"version":"3.55.0"},"reference-count":18,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2014,3,14]],"date-time":"2014-03-14T00:00:00Z","timestamp":1394755200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Acad Dermatol Venereol"],"published-print":{"date-parts":[[2015,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Autologous non\u2010cultured outer root sheath hair follicle cell suspension (<jats:styled-content style=\"fixed-case\">NCORSHFS<\/jats:styled-content>) is a recently described novel cellular graft technique for the treatment of stable vitiligo. There is lack of data about various factors determining the repigmentation rate in vitiligo patients undergoing this novel surgical therapy.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objective<\/jats:title><jats:p>To study the clinical characteristics and treatment variables determining therapeutic outcome in patients of stable vitiligo undergoing <jats:styled-content style=\"fixed-case\">NCORSHFS<\/jats:styled-content>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Non\u2010cultured outer root sheath hair follicle cell suspension was prepared from anagen hairs extracted from the occipital area. The number of melanocytes and hair follicle stem cells (<jats:styled-content style=\"fixed-case\">HFSC<\/jats:styled-content>) in the suspension was quantified by staining with anti\u2010<jats:styled-content style=\"fixed-case\">HMB<\/jats:styled-content>45 and anti\u2010<jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>200 antibody, respectively. In all patients, a 2\u00a0mm punch skin biopsy was taken from one of the vitiligo patch to be treated prior to surgery for assessment of histomorphological features. Post surgery patients were followed up at regular intervals for 24\u00a0weeks.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Thirty patients (21 females, 9 males) with a clinical diagnosis of stable vitiligo, with a total of 60 target lesions were included in this study. The mean age of the study population was 21.10\u00a0\u00b1\u00a05.64\u00a0years. The number of melanocytes (<jats:italic>P\u00a0<\/jats:italic>=\u00a00.04) and <jats:styled-content style=\"fixed-case\">HFSC<\/jats:styled-content> (<jats:italic>P\u00a0<\/jats:italic>=\u00a00.01) transplanted were significantly higher among patients achieving optimum repigmentation (&gt;75% repigmentation). There was a strong correlation between repigmentation at 24\u00a0week and number of melanocytes and <jats:styled-content style=\"fixed-case\">HFSC<\/jats:styled-content> transplanted. Number of <jats:styled-content style=\"fixed-case\">HFSC<\/jats:styled-content> transplanted and absence of dermal inflammation were significant predictors of achieving optimum repigmentation.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The number of melanocytes and <jats:styled-content style=\"fixed-case\">HFSC<\/jats:styled-content> transplanted and absence of dermal inflammation were important determents of optimal repigmentation in patients undergoing <jats:styled-content style=\"fixed-case\">NCORSHFS<\/jats:styled-content> for treatment of stable vitiligo. Hence, refining the technique of <jats:styled-content style=\"fixed-case\">NCORSHFS<\/jats:styled-content> on the basis of these factors would help in achieving better surgical outcomes.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jdv.12426","type":"journal-article","created":{"date-parts":[[2014,3,14]],"date-time":"2014-03-14T08:12:45Z","timestamp":1394784765000},"page":"31-37","source":"Crossref","is-referenced-by-count":40,"title":["Clinical and treatment characteristics determining therapeutic outcome in patients undergoing autologous non\u2010cultured outer root sheath hair follicle cell suspension for treatment of stable vitiligo"],"prefix":"10.1111","volume":"29","author":[{"given":"K.","family":"Vinay","sequence":"first","affiliation":[{"name":"Department of Dermatology Venereology and Leprology Postgraduate Institute of Medical Education and Research Chandigarh India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"Dogra","sequence":"additional","affiliation":[{"name":"Department of Dermatology Venereology and Leprology Postgraduate Institute of Medical Education and Research Chandigarh India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D.","family":"Parsad","sequence":"additional","affiliation":[{"name":"Department of Dermatology Venereology and Leprology Postgraduate Institute of Medical Education and Research Chandigarh India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.J.","family":"Kanwar","sequence":"additional","affiliation":[{"name":"Department of Dermatology Venereology and Leprology Postgraduate Institute of Medical Education and Research Chandigarh India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R.","family":"Kumar","sequence":"additional","affiliation":[{"name":"Department of Dermatology Venereology and Leprology Postgraduate Institute of Medical Education and Research Chandigarh India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R.W.","family":"Minz","sequence":"additional","affiliation":[{"name":"Department of Immunopathology Postgraduate Institute of Medical Education and Research Chandigarh India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"U.N.","family":"Saikia","sequence":"additional","affiliation":[{"name":"Department of Histopathology Postgraduate Institute of Medical Education and Research Chandigarh 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