{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T02:47:26Z","timestamp":1791946046899,"version":"4.3.4"},"reference-count":45,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2014,8,4]],"date-time":"2014-08-04T00:00:00Z","timestamp":1407110400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Acad Dermatol Venereol"],"published-print":{"date-parts":[[2015,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Punch grafting is a surgical technique mainly applied in therapy\u2010resistant, stable and circumscribed vitiligo.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objective<\/jats:title><jats:p>(i) To characterize in detail the features of the repigmented skin among punch grafts; and (ii) to correlate the <jats:italic>ex vivo<\/jats:italic> results with clinical data and punch grafting outcome.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We evaluated by immunohistochemistry and image analysis the expression of a panel of specific melanocyte markers including <jats:styled-content style=\"fixed-case\">HMB<\/jats:styled-content>45, <jats:styled-content style=\"fixed-case\">MITF<\/jats:styled-content>, c\u2010kit, <jats:styled-content style=\"fixed-case\">MART<\/jats:styled-content>\u20101 and <jats:styled-content style=\"fixed-case\">TRP<\/jats:styled-content>1, the proliferation marker Ki67 and the cell\u2013cell adhesion molecule E\u2010cadherin in tissue samples collected from nine patients after punch grafting.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Cells positive for <jats:styled-content style=\"fixed-case\">MITF<\/jats:styled-content>, c\u2010kit, <jats:styled-content style=\"fixed-case\">MART<\/jats:styled-content>\u20101 and <jats:styled-content style=\"fixed-case\">TRP<\/jats:styled-content>1 were detected in the repigmented skin of all biopsies, whereas no reactivity was observed for <jats:styled-content style=\"fixed-case\">HMB<\/jats:styled-content>45. Melanocytes were identified along the entire length of the sections, and their mature state was assessed by the immuno\u2010reactivity for the differentiation marker <jats:styled-content style=\"fixed-case\">MART<\/jats:styled-content>\u20101, the absence of cells positively stained for Ki67 and by the co\u2010expression of c\u2010kit and <jats:styled-content style=\"fixed-case\">TRP<\/jats:styled-content>1, a marker of a differentiated and pigmented state. Clinically, smaller punch grafts aimed at repigmenting lesional areas on the face gave the faster clinical results with no side\u2010effects. Patients subjected to bigger punch grafts on the knee exhibited a longer repigmentation time and presented cobble stoning.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Our results suggest that the repigmentation observed in the areas between the grafts is due to the activation of the melanocytes located in the donor sites. These cells start to horizontally migrate towards the lesional skin thanks to successively the enlargement of intercellular spaces in relation to a decrease of E\u2010cadherin reactivity and the up\u2010modulation of pro\u2010melanogenic mediators. Production and transfer of melanin in the surrounding keratinocytes and their persistence were assessed by the reactivity for <jats:styled-content style=\"fixed-case\">MITF<\/jats:styled-content>, c\u2010kit, <jats:styled-content style=\"fixed-case\">MART<\/jats:styled-content>\u20101 and <jats:styled-content style=\"fixed-case\">TRP<\/jats:styled-content>1 but not for the pre\u2010melanosome marker (<jats:styled-content style=\"fixed-case\">HMB<\/jats:styled-content>45).<\/jats:p><\/jats:sec>","DOI":"10.1111\/jdv.12647","type":"journal-article","created":{"date-parts":[[2014,8,4]],"date-time":"2014-08-04T04:22:11Z","timestamp":1407126131000},"page":"581-590","source":"Crossref","is-referenced-by-count":34,"title":["Vitiligo: characterization of melanocytes in repigmented skin after punch grafting"],"prefix":"10.1111","volume":"29","author":[{"given":"D.","family":"Kovacs","sequence":"first","affiliation":[{"name":"Laboratory of Cutaneous Physiopathology and Integrated Center of 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