{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,20]],"date-time":"2026-09-20T08:33:44Z","timestamp":1789893224683,"version":"4.0.1"},"reference-count":19,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2015,1,22]],"date-time":"2015-01-22T00:00:00Z","timestamp":1421884800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["DFG KA 3438\/1\u20101"],"award-info":[{"award-number":["DFG KA 3438\/1\u20101"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Acad Dermatol Venereol"],"published-print":{"date-parts":[[2016,2]]},"abstract":"Abstract<\/jats:title>Background<\/jats:title>The hormonally active vitamin D metabolite calcitriol and its analogues exert potent effects on cellular differentiation and regulation of immune responses. Although topical vitamin D analogues are widely used for treatment of psoriasis and vitamin D has been increasingly implicated in prevention and protection from several autoimmune diseases, experimental and clinical data in autoimmune bullous diseases are generally lacking.<\/jats:p><\/jats:sec>Objective<\/jats:title>Here, we investigated the effects of calcitriol on keratinocytes treated by bullous pemphigoid (BP<\/jats:styled-content>) autoantibodies.<\/jats:p><\/jats:sec>Methods<\/jats:title>Human keratinocyte (HaCaT) cells were treated with purified human BP<\/jats:styled-content> or normal IgG from one BP<\/jats:styled-content> patient and healthy subject, respectively, in the absence or presence of calcitriol and effects on (i) cell viability, (ii) IL\u20106 and IL\u20108 secretion, (iii) STAT<\/jats:styled-content>3 and NF<\/jats:styled-content>\u03baB activation, (iv) heat shock protein 70 (Hsp70) level, and (v) vitamin D receptor (VDR<\/jats:styled-content>) expression were studied.<\/jats:p><\/jats:sec>Results<\/jats:title>We found that BP<\/jats:styled-content> IgG\u2010induced IL<\/jats:styled-content>\u20106 and IL<\/jats:styled-content>\u20108 release from HaCaT cells was reduced in the presence of non\u2010toxic doses of calcitriol. Additionally, calcitriol blunted BP<\/jats:styled-content> IgG\u2010mediated STAT<\/jats:styled-content>3 phosphorylation and NF<\/jats:styled-content>\u03baB activity, whereas Hsp70 and VDR<\/jats:styled-content> expression were not affected.<\/jats:p><\/jats:sec>Conclusion<\/jats:title>Although the results of this study are based on autoantibodies prepared from a single patient, they show that calcitriol protects from BP<\/jats:styled-content> IgG\u2010induced inflammatory processes in vitro<\/jats:italic>, thus favouring its potential inclusion into the therapeutic repertoire of BP<\/jats:styled-content>.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jdv.12929","type":"journal-article","created":{"date-parts":[[2015,1,22]],"date-time":"2015-01-22T09:35:48Z","timestamp":1421919348000},"page":"288-292","source":"Crossref","is-referenced-by-count":18,"title":["Calcitriol exerts anti\u2010inflammatory effects in keratinocytes treated with autoantibodies from a patient with bullous pemphigoid"],"prefix":"10.1111","volume":"30","author":[{"given":"S.","family":"Tukaj","sequence":"first","affiliation":[{"name":"Department of Dermatology University of L\u00fcbeck L\u00fcbeck Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D.","family":"Gr\u00fcner","sequence":"additional","affiliation":[{"name":"Department of Dermatology University of L\u00fcbeck L\u00fcbeck Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C.","family":"Tukaj","sequence":"additional","affiliation":[{"name":"Department of Electron Microscopy Medical University of Gda\u0144sk Gda\u0144sk Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D.","family":"Zillikens","sequence":"additional","affiliation":[{"name":"Department of Dermatology University of L\u00fcbeck L\u00fcbeck Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Kasperkiewicz","sequence":"additional","affiliation":[{"name":"Department of 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