{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,31]],"date-time":"2026-03-31T08:25:59Z","timestamp":1774945559534,"version":"3.50.1"},"reference-count":30,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2015,1,26]],"date-time":"2015-01-26T00:00:00Z","timestamp":1422230400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"F\u00fcrst Medisinsk Laboratorium"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Acad Dermatol Venereol"],"published-print":{"date-parts":[[2015,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objectives<\/jats:title><jats:p><jats:italic>Mycoplasma genitalium<\/jats:italic> is a common cause of non\u2010gonococcal urethritis (<jats:styled-content style=\"fixed-case\">NGU<\/jats:styled-content>) in Western Europe, but is not routinely tested for in all clinics. A high prevalence of macrolide\u2010resistant <jats:italic>M.\u00a0genitalium<\/jats:italic> has been reported. An easy to use test that can predict likely macrolide treatment failure is potentially very valuable. We report the development of a rapid and reliable real\u2010time <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>\u2010assay which detects all relevant resistance loci in the <jats:italic>M.\u00a0genitalium<\/jats:italic> 23S <jats:styled-content style=\"fixed-case\">rRNA<\/jats:styled-content> gene.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p><jats:italic>Mycoplasma genitalium<\/jats:italic>\u2010positive clinical samples were collected between December 2012 and May 2013, from samples sent routinely to the laboratory for diagnostic testing for <jats:italic>M.\u00a0genitalium<\/jats:italic>. The real\u2010time <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content> assay was designed using forward amplification primers complementary to all relevant commonly identified 23s <jats:styled-content style=\"fixed-case\">rRNA<\/jats:styled-content> gene mutations, a common reverse amplification primer and a common TaqMan Probe.<\/jats:p><\/jats:sec><jats:sec><jats:title>Result<\/jats:title><jats:p>We report a Taqman assay for detection of common 23S <jats:styled-content style=\"fixed-case\">rRNA<\/jats:styled-content> genotypes at position 2058 and 2059 (<jats:italic>Escherichia coli<\/jats:italic> numbering) associated with macrolide resistance, directly from clinical samples. We validated the assay by comparison with <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> sequence determination.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Our TaqMan assay detects common genotypes associated with macrolide\u2010resistant <jats:italic>M.\u00a0genitalium<\/jats:italic>, namely, A2058G, A2059G and A2058C. We show association between the presence of resistant <jats:italic>M.\u00a0genitalium<\/jats:italic> and treatment failure, thereby confirming the validity of testing for these mutants to prevent further spread of antimicrobial resistance and to allow informed choice of antibiotics for treatment.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jdv.12963","type":"journal-article","created":{"date-parts":[[2015,1,26]],"date-time":"2015-01-26T12:25:17Z","timestamp":1422275117000},"page":"1616-1620","source":"Crossref","is-referenced-by-count":45,"title":["Identification of macrolide\u2010resistant <i>Mycoplasma genitalium<\/i> using real\u2010time <scp>PCR<\/scp>"],"prefix":"10.1111","volume":"29","author":[{"given":"C.","family":"Wold","sequence":"first","affiliation":[{"name":"F\u00fcrst Medical Laboratory Oslo Norway"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.","family":"Sorthe","sequence":"additional","affiliation":[{"name":"F\u00fcrst Medical Laboratory Oslo Norway"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"U.","family":"Hartgill","sequence":"additional","affiliation":[{"name":"Olafia Clinic Oslo University Hospital Oslo Norway"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.O.","family":"Olsen","sequence":"additional","affiliation":[{"name":"Olafia Clinic Oslo University Hospital Oslo Norway"},{"name":"Norwegian National Advisory Unit on Sexually Transmitted Infections Oslo Norway"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"Moghaddam","sequence":"additional","affiliation":[{"name":"F\u00fcrst Medical Laboratory Oslo Norway"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"N.","family":"Reinton","sequence":"additional","affiliation":[{"name":"F\u00fcrst Medical Laboratory Oslo Norway"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2015,1,26]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1556\/AMicr.54.2007.1.2"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1136\/sti.79.4.318"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1258\/ijsa.2008.008038"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0035593"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0061481"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/ciu217"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.4045\/tidsskr.10.1224"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/cit752"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.3402\/ijch.v71i0.18203"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1086\/593188"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.3201\/eid1706.101055"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.39.12.2770"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10156-009-0021-4"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.02475-10"},{"key":"e_1_2_5_16_1","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dks263"},{"key":"e_1_2_5_17_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.03318-13"},{"key":"e_1_2_5_18_1","doi-asserted-by":"publisher","DOI":"10.1136\/sti.2008.032748"},{"key":"e_1_2_5_19_1","first-page":"2709","article-title":"Mycoplasma genitalium in men with non\u2010gonococcal urethritis","volume":"128","author":"Moi H","year":"2008","journal-title":"Tidsskr den Nor l\u00e6geforening Tidsskr Prakt Med ny r\u00e6kke"},{"key":"e_1_2_5_20_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.42.3.1236-1242.2004"},{"key":"e_1_2_5_21_1","unstructured":"HornerPJ BleeK FalkL Van der MeijdenW MoiH.European Guideline on the m anagement of non \u2010 gonococcal urethritis [Internet]. 2014. 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