{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,6]],"date-time":"2026-03-06T07:48:57Z","timestamp":1772783337268,"version":"3.50.1"},"reference-count":22,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2015,3,24]],"date-time":"2015-03-24T00:00:00Z","timestamp":1427155200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Regione Piemonte- Progetto per la Ricerca Sanitaria Finalizzata"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Acad Dermatol Venereol"],"published-print":{"date-parts":[[2016,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>No data are available as to the phenotype of circulating lymphocyte subsets in pyoderma gangrenosum (<jats:styled-content style=\"fixed-case\">PG<\/jats:styled-content>).<\/jats:p><\/jats:sec><jats:sec><jats:title>Aim<\/jats:title><jats:p>To analyse the expression of different chemokine receptors associated to T\u2010helper (Th)1 (<jats:styled-content style=\"fixed-case\">CCR<\/jats:styled-content>5), Th2 (<jats:styled-content style=\"fixed-case\">CCR<\/jats:styled-content>4) and Th17 (<jats:styled-content style=\"fixed-case\">CCR<\/jats:styled-content>6), as well as the regulatory T\u2010cell subset (Treg) and dendritic cell polarization in the blood of newly diagnosed untreated <jats:styled-content style=\"fixed-case\">PG<\/jats:styled-content> patients.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and methods<\/jats:title><jats:p>Multi\u2010parameter flow cytometry was performed on blood samples from 10 <jats:styled-content style=\"fixed-case\">PG<\/jats:styled-content> patients collected at first diagnosis among centres belonging to the Italian Immuno\u2010pathology Group. Blood samples from 10 age\u2010 and sex\u2010matched healthy controls (<jats:styled-content style=\"fixed-case\">HC<\/jats:styled-content>) were used as controls.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p><jats:styled-content style=\"fixed-case\">PG<\/jats:styled-content> patients are characterized by an over\u2010expression in the blood of the <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>4+<jats:styled-content style=\"fixed-case\">CCR<\/jats:styled-content>5+ and <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>4+<jats:styled-content style=\"fixed-case\">CCR<\/jats:styled-content>6+ and a down\u2010regulation of <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>4+<jats:styled-content style=\"fixed-case\">CCR<\/jats:styled-content>4+ counts with respect to healthy subjects. Moreover, they show increased levels of myeloid derived dendritic cells type1 and reduced levels of the Treg <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>4+<jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>25high<jats:styled-content style=\"fixed-case\">FOXP<\/jats:styled-content>3+ subset.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The pattern of chemokine expression argues in favour of a Th1 (<jats:styled-content style=\"fixed-case\">CCR<\/jats:styled-content>5+) and Th17 (<jats:styled-content style=\"fixed-case\">CCR<\/jats:styled-content>6+) polarization with a down\u2010regulation of Th2 (<jats:styled-content style=\"fixed-case\">CCR<\/jats:styled-content>4+).<\/jats:p><\/jats:sec>","DOI":"10.1111\/jdv.13100","type":"journal-article","created":{"date-parts":[[2015,3,25]],"date-time":"2015-03-25T06:14:30Z","timestamp":1427264070000},"page":"655-658","source":"Crossref","is-referenced-by-count":19,"title":["Phenotypical characterization of circulating cell subsets in pyoderma gangrenosum patients: the experience of the Italian immuno\u2010pathology group"],"prefix":"10.1111","volume":"30","author":[{"given":"P.","family":"Quaglino","sequence":"first","affiliation":[{"name":"Dermatologic Clinic Department of Medical Sciences University of Turin Turin Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P.","family":"Fava","sequence":"additional","affiliation":[{"name":"Dermatologic Clinic Department of Medical Sciences University of Turin Turin 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