{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,17]],"date-time":"2026-02-17T05:19:01Z","timestamp":1771305541432,"version":"3.50.1"},"reference-count":32,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2015,10,7]],"date-time":"2015-10-07T00:00:00Z","timestamp":1444176000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Acad Dermatol Venereol"],"published-print":{"date-parts":[[2016,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Mucous membrane pemphigoid (<jats:styled-content style=\"fixed-case\">MMP<\/jats:styled-content>) is characterized by subepithelial blistering due to IgG autoantibodies targeting various components of the dermal\u2013epidermal basement membrane zone. Immunodiagnostics play an important role in making a precise diagnosis. Measures of test sensitivity and specificity, however, typically come from studies in diseases such as bullous pemphigoid, where the exact antigenic site may not be the same. Additionally, the association of clinical phenotype and autoantibody profiles has been an area of debate.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objective<\/jats:title><jats:p>We evaluated the sensitivity and specificity of <jats:styled-content style=\"fixed-case\">ELISA<\/jats:styled-content> in <jats:styled-content style=\"fixed-case\">MMP<\/jats:styled-content> for the known target antigens <jats:styled-content style=\"fixed-case\">BP<\/jats:styled-content>180 and laminin\u2010332, as well as characterized the frequency of IgG antibodies against each laminin\u2010332 subdomain. Lastly, we analysed whether IgG auto\u2010antibody profiles were associated with clinical phenotype.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We performed a systematic review of Medline\/PubMed to compile <jats:styled-content style=\"fixed-case\">MMP<\/jats:styled-content> patient demographics, clinical manifestations and immunodiagnostic results. <jats:styled-content style=\"fixed-case\">ELISA<\/jats:styled-content> sensitivities and specificities for autoantigens were calculated in patients with positive immunoblot or immunoprecipitationstudies. IgG reactivity for each laminin\u2010332 subunit was recorded. Associations between positive immunological tests and clinical presentation were evaluated using chi\u2010squared test tests or Fisher's exact test when appropriate.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>For patients with a positive immunoblot or immunoprecipitation to <jats:styled-content style=\"fixed-case\">NC<\/jats:styled-content>16a, <jats:styled-content style=\"fixed-case\">ELISA<\/jats:styled-content> using both the <jats:styled-content style=\"fixed-case\">NC<\/jats:styled-content>16a and C\u2010terminal portion of <jats:styled-content style=\"fixed-case\">BP<\/jats:styled-content>180 demonstrated a sensitivity and specificity of 73% and 93%. However, for individuals with IgG against the C\u2010terminal domain of <jats:styled-content style=\"fixed-case\">BP<\/jats:styled-content>180, the sensitivity and specificity was 43% and 56%, respectively. <jats:styled-content style=\"fixed-case\">LN<\/jats:styled-content>\u2010332 <jats:styled-content style=\"fixed-case\">ELISA<\/jats:styled-content> demonstrated 75% sensitivity, but only for patients with IgG reactivity against the \u03b13 subunit. In patients with laminin\u2010332 <jats:styled-content style=\"fixed-case\">MMP<\/jats:styled-content>, 86.4% demonstrated IgG against the \u03b13 subunit. IgG autoantibodies against laminin\u2010332 are significantly associated with pharyngo\u2010laryngeal (<jats:italic>P<\/jats:italic> &lt; 0.0001) and oro\u2010pharyngo\u2010laryngeal (<jats:italic>P<\/jats:italic> = 0.006) involvement.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Immunodiagnostics play a major role in diagnosing <jats:styled-content style=\"fixed-case\">MMP<\/jats:styled-content>, though limited sensitivity may necessitate several forms of testing for confirmation.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jdv.13397","type":"journal-article","created":{"date-parts":[[2015,10,8]],"date-time":"2015-10-08T03:38:41Z","timestamp":1444275521000},"page":"72-77","source":"Crossref","is-referenced-by-count":43,"title":["A systematic review with pooled analysis of clinical presentation and immunodiagnostic testing in mucous membrane pemphigoid: association of anti\u2010laminin\u2010332 IgG with oropharyngeal involvement and the usefulness of 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