{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,4]],"date-time":"2026-03-04T21:23:02Z","timestamp":1772659382532,"version":"3.50.1"},"reference-count":30,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2015,11,4]],"date-time":"2015-11-04T00:00:00Z","timestamp":1446595200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Almirall, S.A"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Acad Dermatol Venereol"],"published-print":{"date-parts":[[2016,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Reflectance confocal microscopy (RCM) and high\u2010definition optical coherence tomography (HD\u2010OCT) allow the observation <jats:italic>in vivo<\/jats:italic> of dynamic changes in response to non\u2010surgical treatment of actinic keratosis and field of cancerisation.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objective<\/jats:title><jats:p>To non\u2010invasively assess the pharmacodynamic changes induced by treatment with low dose 5\u2010fluorouracil and 10% salicylic acid by means of RCM and high\u2010definition OCT in field cancerization and actinic keratosis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Twenty patients aged &gt;50 years with diagnosis of actinic keratosis on the head and face and indication for treatment with 0.5% 5\u2010fluorouracil and 10% salicylic acid were enrolled. An area of 25 cm\u00b2 including visible type I and II AK and subclinical AK was treated once daily during 6 weeks and examination was performed with RCM and HD\u2010OCT before treatment and 2 weeks after the end of treatment<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>High\u2010definition optical coherence tomography results at baseline of mean thickness of the stratum corneum and epidermis were in AK 10.4 (SD = 4.99) and 43.3 (SD = 24.01) \u03bcm respectively and in subclinical AK 3.7 (SD = 2.15) and 30.05 (SD = 16.85) \u03bcm. At week 8 (2 weeks after the end of treatment) measurements of stratum corneum and epidermis were significantly reduced in AK and subclinical AK for stratum corneum and epidermis. In RCM at week 8 scaling, detached corneocytes, atypical honeycomb, round nucleated cells in the spinosum granulosum layer, round vessels (dermal papillae), inflammatory cells and total AK score were significantly reduced in AK and subclinical AK.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Evaluation of AK and subclinical AK by RCM and HD\u2010OCT showed objective improvement after treatment with 5\u2010fluorouracil and 10% salicylic acid. These methods allowed the study of dynamic changes in the tissue at a subclinical level.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jdv.13445","type":"journal-article","created":{"date-parts":[[2015,11,5]],"date-time":"2015-11-05T03:06:03Z","timestamp":1446692763000},"page":"258-265","source":"Crossref","is-referenced-by-count":29,"title":["Treatment monitoring of 0.5% 5\u2010fluorouracil and 10% salicylic acid in clinical and subclinical actinic keratoses with the combination of optical coherence tomography and reflectance confocal microscopy"],"prefix":"10.1111","volume":"30","author":[{"given":"J.","family":"Malvehy","sequence":"first","affiliation":[{"name":"Melanoma Unit Dermatology Department Hospital Cl\u00ednic Barcelona Spain"},{"name":"Institut d'Investigacions Biom\u00e8diques August Pi I Sunyer (IDIBAPS) Barcelona Spain"},{"name":"Centre on Biomedical Research on Rare Diseases (CIBERER) Instituto Carlos III Barcelona Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"I.","family":"Alarcon","sequence":"additional","affiliation":[{"name":"Melanoma Unit Dermatology Department Hospital Cl\u00ednic Barcelona Spain"},{"name":"Almirall SA Barcelona Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.","family":"Montoya","sequence":"additional","affiliation":[{"name":"Melanoma Unit Dermatology Department Hospital Cl\u00ednic Barcelona Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R.","family":"Rodr\u00edguez\u2010Azeredo","sequence":"additional","affiliation":[{"name":"Almirall SA Barcelona Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.","family":"Puig","sequence":"additional","affiliation":[{"name":"Melanoma Unit Dermatology Department Hospital Cl\u00ednic Barcelona Spain"},{"name":"Institut d'Investigacions Biom\u00e8diques August Pi I Sunyer (IDIBAPS) Barcelona Spain"},{"name":"Centre on Biomedical Research on Rare Diseases (CIBERER) Instituto Carlos III Barcelona Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2015,11,4]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0190-9622(97)70265-2"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/jid.2013.134"},{"key":"e_1_2_6_4_1","first-page":"201","article-title":"Progression of actinic keratosis to squamous cell carcinoma revisited: clinical and treatment implications","volume":"87","author":"Feldman SR","year":"2011","journal-title":"Cutis"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1067\/mjd.2000.103344"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/1097-0142(195309)6:5<963::AID-CNCR2820060515>3.0.CO;2-Q"},{"key":"e_1_2_6_7_1","first-page":"1727","article-title":"A genetic explanation of Slaughter's concept of field cancerization: evidence and clinical implications","volume":"63","author":"Braakhuis BJ","year":"2003","journal-title":"Cancer Res"},{"key":"e_1_2_6_8_1","first-page":"1523","article-title":"Persistence of genetically altered fields in head and neck cancer patients: biological and clinical implications","volume":"7","author":"Tabor MP","year":"2001","journal-title":"Clin Cancer Res"},{"key":"e_1_2_6_9_1","first-page":"2718","article-title":"Detection of minimal residual cancer to investigate why oral tumours recur despite seemingly adequate treatment","volume":"6","author":"Partridge M","year":"2000","journal-title":"Clin Cancer Res"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1524-4725.2007.34117.x"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1468-3083.2009.03410.x"},{"key":"e_1_2_6_12_1","first-page":"2302","article-title":"Reflectance confocal microscopy: non\u2010invasive distinction between actinic keratosis and squamous cell carcinoma","volume":"2","author":"Peppelman M","year":"2015","journal-title":"J Eur Acad Dermatol Venereol"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/exd.12074"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1067\/mjd.2000.105565"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2133.2007.07865.x"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1159\/000254893"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.2340\/00015555-1860"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/bjd.13304"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/0190-9622(91)70113-G"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/1523-1747.ep12606215"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1001\/archdermatol.2009.134"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2133.2012.11202.x"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2133.2011.10387.x"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1111\/jdv.12702"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1586\/14737167.2015.995171"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1159\/000370148"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1111\/jdv.12848"},{"key":"e_1_2_6_28_1","first-page":"651","article-title":"Development of a treatment algorithm for actinic keratoses: a European Consensus","volume":"18","author":"Stockfleth E","year":"2008","journal-title":"Eur J Dermatol"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1111\/jdv.12833"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1111\/jdv.12954"},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaad.2014.04.007"}],"container-title":["Journal of the European Academy of Dermatology and Venereology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fjdv.13445","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fjdv.13445","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jdv.13445","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,24]],"date-time":"2023-09-24T15:52:31Z","timestamp":1695570751000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/jdv.13445"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,11,4]]},"references-count":30,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2016,2]]}},"alternative-id":["10.1111\/jdv.13445"],"URL":"https:\/\/doi.org\/10.1111\/jdv.13445","archive":["Portico"],"relation":{},"ISSN":["0926-9959","1468-3083"],"issn-type":[{"value":"0926-9959","type":"print"},{"value":"1468-3083","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,11,4]]}}}