{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,2]],"date-time":"2026-06-02T15:19:36Z","timestamp":1780413576522,"version":"3.54.1"},"reference-count":20,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2016,2,11]],"date-time":"2016-02-11T00:00:00Z","timestamp":1455148800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Polish Ministry of Science and Higher Education","award":["NN 404\/4942\/38"],"award-info":[{"award-number":["NN 404\/4942\/38"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Acad Dermatol Venereol"],"published-print":{"date-parts":[[2016,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Hyperkeratosis of skin in lower limb lymphedema is one of the sequelae of tissue fluid\/lymph (<jats:styled-content style=\"fixed-case\">TF<\/jats:styled-content>\/L) stasis, but its mechanisms remain unknown. It is noteworthy, nonetheless, that human <jats:styled-content style=\"fixed-case\">TF<\/jats:styled-content>\/L contains high levels of growth factors and cytokines, and may serve as the physiological environment for keratinocyte (<jats:styled-content style=\"fixed-case\">KC<\/jats:styled-content>) proliferation.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objective<\/jats:title><jats:p>The aim of the study was to investigate the effect of human <jats:styled-content style=\"fixed-case\">TF<\/jats:styled-content>\/L on human <jats:styled-content style=\"fixed-case\">KC<\/jats:styled-content> proliferation, differentiation and on the expression of epidermal stem cell markers on them.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p><jats:styled-content style=\"fixed-case\">KC<\/jats:styled-content> were isolated from lymphedema and normal skin, and cultured for 1\u201314 days in <jats:styled-content style=\"fixed-case\">TF<\/jats:styled-content>\/L with neutralized Interleukin 1\u03b2, Interleukin 6, tumour necrosis factor \u03b1 (<jats:styled-content style=\"fixed-case\">TNF<\/jats:styled-content>\u2010\u03b1), keratinocyte growth factor (<jats:styled-content style=\"fixed-case\">KGF<\/jats:styled-content>) or tumour growth factor \u03b2 (<jats:styled-content style=\"fixed-case\">TGF<\/jats:styled-content>\u2010\u03b2). Alternatively, <jats:styled-content style=\"fixed-case\">KC<\/jats:styled-content> receptors for these factors were blocked.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The number of <jats:styled-content style=\"fixed-case\">KC<\/jats:styled-content> cultured in <jats:styled-content style=\"fixed-case\">TF<\/jats:styled-content>\/L was increased, as was the percentage of mitotic figures. There was a higher percentage of p63, <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>29, Ki67, <jats:styled-content style=\"fixed-case\">PCNA<\/jats:styled-content>,<jats:styled-content style=\"fixed-case\"> CK<\/jats:styled-content>6, <jats:styled-content style=\"fixed-case\">CK<\/jats:styled-content>17, <jats:styled-content style=\"fixed-case\">CK<\/jats:styled-content>16 and a lower of <jats:styled-content style=\"fixed-case\">CK<\/jats:styled-content>10, <jats:styled-content style=\"fixed-case\">CK<\/jats:styled-content>14, filaggrin and involucrin\u2010positive <jats:styled-content style=\"fixed-case\">KC<\/jats:styled-content>. Neutralization of <jats:styled-content style=\"fixed-case\">TF<\/jats:styled-content>\/L <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u20101\u03b2, <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u20106, <jats:styled-content style=\"fixed-case\">TNF<\/jats:styled-content>\u2010\u03b1 and <jats:styled-content style=\"fixed-case\">KGF<\/jats:styled-content> as well as blockage of their receptors resulted in decreased percentage of mitotic <jats:styled-content style=\"fixed-case\">KC<\/jats:styled-content>. <jats:styled-content style=\"fixed-case\">TGF<\/jats:styled-content>\u2010\u03b2 had a limited effect on <jats:styled-content style=\"fixed-case\">KC<\/jats:styled-content> proliferation.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Hyperkeratosis in lymphedema may be the effect of a high concentration of cytokines in the stagnant <jats:styled-content style=\"fixed-case\">TF<\/jats:styled-content>\/L tissue, but not because of presumed changes in the <jats:styled-content style=\"fixed-case\">KC<\/jats:styled-content>.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jdv.13565","type":"journal-article","created":{"date-parts":[[2016,2,12]],"date-time":"2016-02-12T08:11:49Z","timestamp":1455264709000},"page":"1002-1008","source":"Crossref","is-referenced-by-count":14,"title":["Hyperkeratosis in human lower limb lymphedema: the effect of stagnant tissue fluid\/lymph"],"prefix":"10.1111","volume":"30","author":[{"given":"A.","family":"Domaszewska\u2010Szostek","sequence":"first","affiliation":[{"name":"Department of Transplantation Surgery and Department of Human Epigenetics Mossakowski Medical Research Centre Polish Academy of Sciences Warsaw Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Zaleska","sequence":"additional","affiliation":[{"name":"Department of Transplantation Surgery and Department of Human Epigenetics Mossakowski Medical Research Centre Polish Academy 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