{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,12]],"date-time":"2026-05-12T19:30:50Z","timestamp":1778614250151,"version":"3.51.4"},"reference-count":28,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2016,9,5]],"date-time":"2016-09-05T00:00:00Z","timestamp":1473033600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100005230","name":"Natural Science Foundation of Chongqing","doi-asserted-by":"publisher","award":["cstc2015jcyjA10118"],"award-info":[{"award-number":["cstc2015jcyjA10118"]}],"id":[{"id":"10.13039\/501100005230","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Acad Dermatol Venereol"],"published-print":{"date-parts":[[2017,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>The underlying causes and factors contributing to the disease severity of chronic spontaneous urticaria (<jats:styled-content style=\"fixed-case\">CSU<\/jats:styled-content>) are unknown.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objective<\/jats:title><jats:p>Given the important role of basophils in the pathogenesis of urticaria and that <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>63 serves as a useful marker for basophil activation and detecting, <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>63 expression of basophils is a reliable tool for diagnosing allergy and hypersensitivity reactions to different allergens; the objective of this study was to investigate whether the level of basophil <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>63 expression is correlated with allergen sensitization, serum autoreactivity and basophil reactivity in patients with <jats:styled-content style=\"fixed-case\">CSU<\/jats:styled-content>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Basophil\u2010enriched leucocytes were separated from the blood of 64 patients with chronic urticaria (54 <jats:styled-content style=\"fixed-case\">CSU<\/jats:styled-content> patients and 10 symptomatic dermographism patients), 18 healthy control subjects and seven atopic donors without urticaria. Flow cytometry was then used to detect <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>63 expression on the cell membrane of basophils from all samples. Analysis was also preformed on basophils incubated with sera from <jats:styled-content style=\"fixed-case\">CSU<\/jats:styled-content> patients with positive or negative autologous serum skin test (<jats:styled-content style=\"fixed-case\">ASST<\/jats:styled-content>).<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p><jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>63 expression was significantly higher in the basophils from patients with <jats:styled-content style=\"fixed-case\">CSU<\/jats:styled-content> than in those from patients with symptomatic dermographism and the healthy control group. The levels of <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>63 expression in <jats:styled-content style=\"fixed-case\">CSU<\/jats:styled-content> patients with <jats:styled-content style=\"fixed-case\">ASST<\/jats:styled-content><jats:sup>+<\/jats:sup> and\/or allergen sensitization were higher than those with <jats:styled-content style=\"fixed-case\">ASST<\/jats:styled-content><jats:sup>\u2212<\/jats:sup> and\/or no allergen sensitization patients. Incubation with <jats:styled-content style=\"fixed-case\">ASST<\/jats:styled-content><jats:sup>+<\/jats:sup> serum resulted in an increased expression of <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>63 in the basophils of <jats:styled-content style=\"fixed-case\">ASST<\/jats:styled-content><jats:sup>+<\/jats:sup> <jats:styled-content style=\"fixed-case\">CSU<\/jats:styled-content> patients, whereas no such response was observed in healthy controls or <jats:styled-content style=\"fixed-case\">ASST<\/jats:styled-content><jats:sup>\u2212<\/jats:sup> <jats:styled-content style=\"fixed-case\">CSU<\/jats:styled-content> patients.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The increased <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>63 expression in basophils from <jats:styled-content style=\"fixed-case\">CSU<\/jats:styled-content> patients may correlate with allergen sensitization, autoreactivity of serum and basophil reactivity. Our results suggest that <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>63 may contribute new insight into the pathogenesis of <jats:styled-content style=\"fixed-case\">CSU<\/jats:styled-content>.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jdv.13912","type":"journal-article","created":{"date-parts":[[2016,8,12]],"date-time":"2016-08-12T14:58:35Z","timestamp":1471013915000},"page":"463-468","source":"Crossref","is-referenced-by-count":17,"title":["Basophil CD63 expression in chronic spontaneous urticaria: correlation with allergic sensitization, serum autoreactivity and basophil reactivity"],"prefix":"10.1111","volume":"31","author":[{"given":"Q.","family":"Chen","sequence":"first","affiliation":[{"name":"Department of Dermatology Southwest Hospital Third Military Medical University Chongqing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Z.","family":"Zhai","sequence":"additional","affiliation":[{"name":"Department of Dermatology Southwest Hospital Third Military Medical University Chongqing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Dermatology Southwest Hospital Third Military Medical University Chongqing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"W.","family":"Chen","sequence":"additional","affiliation":[{"name":"IZZ Immunologie\u2010Zentrum Z\u00fcrich Z\u00fcrich Switzerland"},{"name":"Department of Dermatology and Allergy Technische Universit\u00e4t M\u00fcnchen Munich Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Dermatology Southwest Hospital Third Military Medical University Chongqing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H.","family":"Zhong","sequence":"additional","affiliation":[{"name":"Department of Dermatology Southwest Hospital Third 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