{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,20]],"date-time":"2026-09-20T10:39:39Z","timestamp":1789900779173,"version":"4.0.1"},"reference-count":23,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2017,8,6]],"date-time":"2017-08-06T00:00:00Z","timestamp":1501977600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["26860895"],"award-info":[{"award-number":["26860895"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Acad Dermatol Venereol"],"published-print":{"date-parts":[[2017,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Accumulating evidence suggests that the lipid lytic enzyme monoacylglycerol lipase (<jats:styled-content style=\"fixed-case\">MAGL<\/jats:styled-content>) promotes tumour invasion and metastasis through up\u2010regulation of pro\u2010tumorigenic signalling lipids in several tumour cell lines. However, the expression status of <jats:styled-content style=\"fixed-case\">MAGL<\/jats:styled-content> in clinical melanoma tissues and its clinicopathological significance remain unclear.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objective<\/jats:title><jats:p>To correlate the tumour expression status of <jats:styled-content style=\"fixed-case\">MAGL<\/jats:styled-content> with the clinicopathological information of patients with malignant melanoma.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Polymerase chain reaction (<jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>) array screening was performed, and the results were validated using immunocytochemical analysis of tumour and non\u2010tumour melanocytic cell lines. Immunohistochemical staining for <jats:styled-content style=\"fixed-case\">MAGL<\/jats:styled-content> was performed for 74 melanoma samples, including 48 primary and 26 metastatic tumours, in which the expression of <jats:styled-content style=\"fixed-case\">MAGL<\/jats:styled-content> was determined by evaluating the percentage of <jats:styled-content style=\"fixed-case\">MAGL<\/jats:styled-content>\u2010positive tumour cells and the <jats:styled-content style=\"fixed-case\">MAGL<\/jats:styled-content> staining intensity. Finally, we analysed the association of <jats:styled-content style=\"fixed-case\">MAGL<\/jats:styled-content> expression status with tumour progression, tumour thickness and vascular invasion of the primary lesion.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Immunocytochemical analysis revealed that <jats:styled-content style=\"fixed-case\">MAGL<\/jats:styled-content> was expressed in all 12 melanoma cell lines, but not in normal human epidermal melanocytes. In the immunohistochemical analysis, positive staining for <jats:styled-content style=\"fixed-case\">MAGL<\/jats:styled-content> was noted in 32 of 48 (64.5%) primary lesions, 14 of 17 (82.4%) lymph node metastatic lesions and 7 of 9 (77.8%) skin metastatic lesions. Metastatic tumours had a significantly higher staining intensity (<jats:italic>P<\/jats:italic> = 0.033 for lymph node, <jats:italic>P<\/jats:italic> = 0.010 for skin). In the analysis of primary lesions, higher <jats:styled-content style=\"fixed-case\">MAGL<\/jats:styled-content> expression correlated with greater tumour thickness (<jats:italic>P<\/jats:italic> = 0.015) and the presence of vascular invasion (<jats:italic>P<\/jats:italic> = 0.017). On further evaluation of <jats:styled-content style=\"fixed-case\">MAGL<\/jats:styled-content>\u2010positive primary lesions, staining intensity of <jats:styled-content style=\"fixed-case\">MAGL<\/jats:styled-content> tended to be higher in deeper areas of the tumour mass.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The expression of <jats:styled-content style=\"fixed-case\">MAGL<\/jats:styled-content> in tumour cells reflects the aggressiveness of melanoma cells and may serve as a marker of tumour progression.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jdv.14455","type":"journal-article","created":{"date-parts":[[2017,7,6]],"date-time":"2017-07-06T05:02:40Z","timestamp":1499317360000},"page":"2038-2045","source":"Crossref","is-referenced-by-count":38,"title":["Expression of monoacylglycerol lipase as a marker of tumour invasion and progression in malignant melanoma"],"prefix":"10.1111","volume":"31","author":[{"given":"Yuko","family":"Baba","sequence":"first","affiliation":[{"name":"Department of Dermatology Keio University School of Medicine Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T.","family":"Funakoshi","sequence":"additional","affiliation":[{"name":"Department of Dermatology Keio University School of Medicine Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Mori","sequence":"additional","affiliation":[{"name":"Department of Dermatology Keio University School of Medicine Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K.","family":"Emoto","sequence":"additional","affiliation":[{"name":"Department of Pathology Keio University School of Medicine Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Y.","family":"Masugi","sequence":"additional","affiliation":[{"name":"Department of Pathology Keio University School of Medicine Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"Ekmekcioglu","sequence":"additional","affiliation":[{"name":"Department of Melanoma Medical Oncology University of Texas MD Anderson Cancer Center Houston TX USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Amagai","sequence":"additional","affiliation":[{"name":"Department of Dermatology Keio University School of Medicine Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K.","family":"Tanese","sequence":"additional","affiliation":[{"name":"Department of Dermatology Keio University School of Medicine Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,8,6]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2001.19.16.3635"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.6004\/jnccn.2016.0051"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1097\/00000658-197011000-00017"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.lfs.2012.10.025"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.2174\/092986710791859414"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12013-014-9899-2"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2009.11.027"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chembiol.2011.05.009"},{"key":"e_1_2_5_10_1","unstructured":"Research Definiens.Tissue Studio. 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