{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T20:26:15Z","timestamp":1782937575543,"version":"3.54.5"},"reference-count":37,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2018,2,27]],"date-time":"2018-02-27T00:00:00Z","timestamp":1519689600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Acad Dermatol Venereol"],"published-print":{"date-parts":[[2018,6]]},"abstract":"Abstract<\/jats:title>Background<\/jats:title>Polymorphic light eruption (PLE<\/jats:styled-content>) is the most common autoimmune photodermatosis. Plasmacytoid dendritic cells (PDC<\/jats:styled-content>s) are important mediators of innate antimicrobial immunity involved in the pathogenesis of many inflammatory skin diseases. In addition to PDC<\/jats:styled-content>s, regulatory T cells (Tregs) are involved in controlling inflammation and adaptive immunity in skin by their immunosuppressive capacity.<\/jats:p><\/jats:sec>Objective<\/jats:title>The aim of this study was to investigate the presence of PDC<\/jats:styled-content>s and Tregs in photoexposed skin from PLE<\/jats:styled-content> compared to healthy skin.<\/jats:p><\/jats:sec>Methods<\/jats:title>Patients with PLE<\/jats:styled-content> diagnosis and healthy controls were recruited and underwent a photoprovocative test. A 4\u2010mm punch biopsy was taken from the site of positive photoprovocation test reaction, and immunohistochemistry for BDCA<\/jats:styled-content>2 as marker for PDC<\/jats:styled-content>s, CD<\/jats:styled-content>4 and FOXP<\/jats:styled-content>3 as markers for Tregs was performed. Double immunostain for FOXP<\/jats:styled-content>3 and CD<\/jats:styled-content>4 was performed as well. Absolute counts for CD<\/jats:styled-content>4, BDCA<\/jats:styled-content>2 and FOXP<\/jats:styled-content>3 were performed in at least 5 High Power Fields (HPF<\/jats:styled-content>). Percentage of CD<\/jats:styled-content>4\u2010, BDCA<\/jats:styled-content>2\u2010 and CD<\/jats:styled-content>4FOXP<\/jats:styled-content>3\u2010positive cells over the total inflammatory infiltrate was assessed for each case.<\/jats:p><\/jats:sec>Results<\/jats:title>We enrolled 23 patients and controls. BDCA<\/jats:styled-content>2+ cells were present in 91.3% of PLE<\/jats:styled-content> skin samples and 100% of healthy volunteer. Both in PLE<\/jats:styled-content> patients and healthy controls, PDC<\/jats:styled-content>s distribution was mainly dermic (P<\/jats:italic> < 0.05). Compared to healthy controls, both epidermic and dermic BDCA<\/jats:styled-content>2+ cells count were significantly higher in PLE<\/jats:styled-content> patients (P<\/jats:italic> < 0.05). Both in PLE<\/jats:styled-content> patients and healthy controls, Tregs distribution was mainly dermic (P<\/jats:italic> < 0.05). The presence of both CD<\/jats:styled-content>4+ cells and FOXP<\/jats:styled-content>3+ cells was significantly higher in the dermis of PLE<\/jats:styled-content> patients compared to controls (P<\/jats:italic> < 0.05). Relative percentages of cellular infiltrations confirmed these results.<\/jats:p><\/jats:sec>Conclusions<\/jats:title>D\u2010PDCS<\/jats:styled-content> and Tregs may play a significant role in the development of PLE<\/jats:styled-content>, and dermal distribution of PDC<\/jats:styled-content>s in PLE<\/jats:styled-content> skin biopsies seems to confirm a possible overlap with cutaneous lupus erythematosus (CLE<\/jats:styled-content>).<\/jats:p><\/jats:sec>","DOI":"10.1111\/jdv.14866","type":"journal-article","created":{"date-parts":[[2018,2,11]],"date-time":"2018-02-11T14:32:34Z","timestamp":1518359554000},"page":"985-991","source":"Crossref","is-referenced-by-count":14,"title":["Cutaneous infiltration of plasmacytoid dendritic cells and T regulatory cells in skin lesions of polymorphic light eruption"],"prefix":"10.1111","volume":"32","author":[{"given":"M.T.","family":"Rossi","sequence":"first","affiliation":[{"name":"Dermatology Department ASST Spedali Civili di Brescia University 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