{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,10]],"date-time":"2026-03-10T00:37:19Z","timestamp":1773103039188,"version":"3.50.1"},"reference-count":42,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2018,3,26]],"date-time":"2018-03-26T00:00:00Z","timestamp":1522022400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Acad Dermatol Venereol"],"published-print":{"date-parts":[[2018,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Patch test (PT) is the gold standard to reveal nickel (Ni) allergy. <jats:italic>In vitro<\/jats:italic> tests are under discussion. We aimed to establish a cytokine based <jats:italic>in\u00a0vitro<\/jats:italic> assay to detect Ni sensitization.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>From 15 patients with positive (13f, 42\u201378\u00a0years) and 15 with negative PT to Ni (controls, 3f, 31\u201382\u00a0years) within a consecutive patient series, peripheral blood mononuclear cells (PBMC) were obtained. Six\u2010day stimulation with three concentrations of NiSO<jats:sub>4<\/jats:sub> was performed. Proliferative response (uptake of tritiated thymidine) and production of the cytokines IL\u20101\u03b2, IL\u20104, IL\u20105, IL\u20106, IL\u20108, IL\u201010, IL\u201017A, TNF\u2010\u03b1, IFN\u2010\u03b3 (cytometric bead array) were assessed.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Twelve of fifteen Ni PT\u2010positive patients also had positive LTT, and all control patients were PT and LTT negative. The mean SI differed between Ni allergics and controls (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.01). Upon Ni stimulation, PBMC of the allergic patients showed (i) enhanced IL\u20105 response (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.0001) and (ii) reduced IL\u20108 production (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.01). The IL\u20105\/IL\u20108\u2010ratio best distinguished allergics from non\u2010allergics in all three Ni concentrations with a sensitivity and specificity of 93%.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Assessment of the ratio of Ni\u2010induced IL\u20105 and reduced IL\u20108 production <jats:italic>in\u00a0vitro<\/jats:italic> is a helpful tool for a better characterization of Ni\u2010allergic patients.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jdv.14931","type":"journal-article","created":{"date-parts":[[2018,3,10]],"date-time":"2018-03-10T07:12:36Z","timestamp":1520665956000},"page":"1542-1548","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Cytokine patterns <i>in\u00a0vitro<\/i>, in particular IL\u20105\/IL\u20108 ratio, to detect patients with nickel contact allergy"],"prefix":"10.1111","volume":"32","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8136-7335","authenticated-orcid":false,"given":"B.","family":"Summer","sequence":"first","affiliation":[{"name":"Department of Dermatology and Allergology Ludwig\u2010Maximilians\u2010University Munich Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.","family":"St\u00e4nder","sequence":"additional","affiliation":[{"name":"Department of Dermatology and Allergology Ludwig\u2010Maximilians\u2010University Munich Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P.","family":"Thomas","sequence":"additional","affiliation":[{"name":"Department of Dermatology and Allergology Ludwig\u2010Maximilians\u2010University Munich Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2018,3,26]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1398-9995.2001.00086.x"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0536.2007.01220.x"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0190-9622(89)70329-7"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.0105-1873.2004.00351.x"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/cod.12563"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2222.1997.tb00690.x"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1398-9995.2004.00547.x"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2222.2004.02045.x"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1159\/000237610"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/ni.1919"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2013.03.023"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/cod.12751"},{"key":"e_1_2_5_14_1","first-page":"2090","article-title":"The \u2018memory lymphocyte immunostimulation assay\u2019 (MELISA) is useless for the detection of metal allergy","volume":"149","author":"Koene RA","year":"2005","journal-title":"Ned Tijdschr Geneeskd"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.2332\/allergolint.O-06-457"},{"key":"e_1_2_5_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.b.20166"},{"key":"e_1_2_5_17_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2133.2002.04850.x"},{"key":"e_1_2_5_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0536.2010.01744.x"},{"key":"e_1_2_5_19_1","doi-asserted-by":"publisher","DOI":"10.1159\/000074905"},{"key":"e_1_2_5_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2249.2006.03226.x"},{"key":"e_1_2_5_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tox.2004.12.026"},{"key":"e_1_2_5_22_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0536.2007.01045.x"},{"key":"e_1_2_5_23_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3083.2005.01673.x"},{"key":"e_1_2_5_24_1","doi-asserted-by":"publisher","DOI":"10.1111\/jdv.12005"},{"key":"e_1_2_5_25_1","doi-asserted-by":"publisher","DOI":"10.1159\/000456031"},{"key":"e_1_2_5_26_1","first-page":"37","article-title":"Contact allergy to nickel: patch test score correlates with IL\u20105, but not with IFN\u2010gamma nickel\u2010specific secretion by peripheral blood lymphocytes","volume":"16","author":"Czarnobilska E","year":"2009","journal-title":"Ann Agric Environ Med"},{"key":"e_1_2_5_27_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1610-0387.2009.06787.x"},{"key":"e_1_2_5_28_1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1398-9995.2001.056008767.x"},{"key":"e_1_2_5_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0190-9622(94)70220-9"},{"key":"e_1_2_5_30_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0536.2010.01702.x"},{"key":"e_1_2_5_31_1","doi-asserted-by":"publisher","DOI":"10.1111\/cod.12465"},{"key":"e_1_2_5_32_1","doi-asserted-by":"publisher","DOI":"10.1111\/1523-1747.ep12494841"},{"key":"e_1_2_5_33_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2222.2003.01700.x"},{"key":"e_1_2_5_34_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2222.2002.01382.x"},{"key":"e_1_2_5_35_1","doi-asserted-by":"publisher","DOI":"10.1097\/ACI.0b013e328357b05a"},{"key":"e_1_2_5_36_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2133.2004.05828.x"},{"key":"e_1_2_5_37_1","doi-asserted-by":"publisher","DOI":"10.1155\/2013\/539834"},{"key":"e_1_2_5_38_1","doi-asserted-by":"publisher","DOI":"10.1002\/tox.22016"},{"key":"e_1_2_5_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.taap.2013.09.014"},{"key":"e_1_2_5_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cellimm.2013.04.003"},{"key":"e_1_2_5_41_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00281-016-0607-8"},{"key":"e_1_2_5_42_1","doi-asserted-by":"publisher","DOI":"10.1038\/jid.2014.61"},{"key":"e_1_2_5_43_1","doi-asserted-by":"publisher","DOI":"10.1097\/DER.0000000000000159"}],"container-title":["Journal of the European Academy of Dermatology and Venereology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fjdv.14931","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jdv.14931","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,17]],"date-time":"2023-09-17T17:32:38Z","timestamp":1694971958000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/jdv.14931"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,3,26]]},"references-count":42,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2018,9]]}},"alternative-id":["10.1111\/jdv.14931"],"URL":"https:\/\/doi.org\/10.1111\/jdv.14931","archive":["Portico"],"relation":{},"ISSN":["0926-9959","1468-3083"],"issn-type":[{"value":"0926-9959","type":"print"},{"value":"1468-3083","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,3,26]]},"assertion":[{"value":"2017-09-07","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-02-08","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-03-26","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}